Method and reagent kit used for detecting low-density lipoprotein cholesterin

A low-density lipoprotein and cholesterol technology, applied in the field of biochemistry, can solve the problems of complex, cumbersome detection steps, unfavorable rapid detection, etc., and achieve the effect of high accuracy and precision, and simple detection.

Active Publication Date: 2013-05-15
CHANGCHUN HUILI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this precipitant method needs to remove globulin, high-density lipoprotein and other substances that interfere with the detection in the blood sample, making the detection steps cumbersome and complicated, which is not conducive to rapid detection

Method used

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  • Method and reagent kit used for detecting low-density lipoprotein cholesterin
  • Method and reagent kit used for detecting low-density lipoprotein cholesterin
  • Method and reagent kit used for detecting low-density lipoprotein cholesterin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1: method of the present invention

[0023] Make a mixed solution of phosphotungstic acid and agar gel (the concentration of phosphotungstic acid is 4.4g / L, the concentration of agar gel is 0.25g / L, pH 6.1), in the magnesium chloride solution with a concentration of 3g / L, the sample to be tested is mixed with Solution and Polyethylene Glycol 20000 (8g / L) are mixed according to volume ratio, test sample: mixed solution: Polyethylene Glycol 20000=1: 48: 12, and the suspension liquid obtained detects absorbance A at 630nm wavelength 样 , then with the A 标 Turbidity, calculate the LDL-C concentration of the sample to be tested, the formula is sample LDL-C concentration (mmol / L) = standard solution concentration (2.6mmol / L) × A 样 / A 标 .

Embodiment 2

[0024] Embodiment 2: method of the present invention

[0025] Make a mixed solution of phosphotungstic acid and agar gel (the concentration of phosphotungstic acid is 4g / L, the concentration of agar gel is 0.3g / L, pH 6.2), in the magnesium chloride solution with a concentration of 2g / L, the sample to be tested and the mixed solution Mix with Polyethylene Glycol 20000 (6g / L) by volume, sample to be tested: mixed solution: Polyethylene Glycol 20000=1: 48: 12, and the suspension obtained detects the absorbance A at a wavelength of 630nm 样 , then with the A 标 Turbidity, calculate the LDL-C concentration of the sample to be tested, the formula is sample LDL-C concentration (mmol / L) = standard solution concentration (2.6mmol / L) × A 样 / A 标 .

Embodiment 3

[0026] Embodiment 3: method of the present invention

[0027]Make a mixed solution of phosphotungstic acid and agar gel (the concentration of phosphotungstic acid is 5g / L, the concentration of agar gel is 0.4g / L, pH 6.1), in the magnesium chloride solution with a concentration of 4g / L, the sample to be tested and the mixed solution Mix with Polyethylene Glycol 20000 (10g / L) by volume, sample to be tested: mixed solution: Polyethylene Glycol 20000=1: 48: 12, and the suspension obtained detects the absorbance A at a wavelength of 630nm 样 , then with the A 标 Turbidity, calculate the LDL-C concentration of the sample to be tested, the formula is sample LDL-C concentration (mmol / L) = standard solution concentration (2.6mmol / L) × A 样 / A 标 .

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Abstract

The invention relates to the filed of biochemistry, and discloses a method and a reagent kit used for detecting low-density lipoprotein cholesterin. The method comprises the steps of: combining phosphotungstic acid and agaropectin into a mixed liquid with the pH value of 6.1-6.2; mixing a sample to be tested with the mixed liquid and polyethylene glycol 20000 under the action of magnesium ions toobtain a suspension turbid liquid; then detecting light absorbance under a wavelength of 630nm; and calculating the concentration of low-density lipoprotein cholesterin of the sample to be tested through turbidimetry with a standard solution subjected to the same treatment abovementioned. The reagent kit disclosed by the invention comprises 2-4g / L magnesium chloride, 6-10g / L polyethylene glycol 20000, 4-5g / L phosphotungstic acid and 0.2-0.4g / L agaropectin. According to the method disclosed by the invention, the specific reaction with low-density lipoprotein without removing impure proteins can be realized, so that the detection of the low-density lipoprotein cholesterin is simple, quick and accurate, and the method can be widely applied to the detection of low-density lipoprotein cholesterin.

Description

technical field [0001] The invention relates to the field of biochemistry, in particular to a method and a kit for detecting low-density lipoprotein cholesterol. Background technique [0002] Cholesterol is an indispensable and important substance for the human body. It not only participates in the formation of plasma membrane, but also is the raw material for the synthesis of bile acid, vitamin D and steroid hormones. In normal blood, cholesterol is mainly combined with low-density lipoprotein (LDL) and circulates in the blood in the form of lipoprotein. The combined cholesterol is called low-density lipoprotein cholesterol (LDL-C). When cells need to use cholesterol to synthesize plasma membrane When waiting for the substance, the cell makes the LDL receptor protein and installs it on the plasma membrane. LDL binds to cell surface receptors and fuses with lysosomes to hydrolyze the cholesterol on LDL for the synthesis of substances such as plasma membranes. [0003] With...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/31
Inventor 董理
Owner CHANGCHUN HUILI BIOTECH CO LTD
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