Preparation method of silk fibroin/hydroxyapatite/collagen composite porous stent

A technology of hydroxyapatite and silk fibroin, applied in the field of materials, can solve the problems of time-consuming and complicated operation, and achieve the effects of good pore connectivity, high porosity, moderate water absorption and expansion rate

Inactive Publication Date: 2012-12-12
SHAANXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these two methods have disadvantages such as complicated operation and time-consuming

Method used

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  • Preparation method of silk fibroin/hydroxyapatite/collagen composite porous stent
  • Preparation method of silk fibroin/hydroxyapatite/collagen composite porous stent
  • Preparation method of silk fibroin/hydroxyapatite/collagen composite porous stent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] 1. Preparation of silk fibroin aqueous solution

[0044] Take 20 g of silkworm cocoons from which pupae have been removed, add 0.02mol / L Na 2 CO 3 2000g of aqueous solution, boiled for 30 minutes, took out and wrung out, rinsed with distilled water; repeated the above operation twice, and dried in vacuum at 50°C for 12 hours to obtain degummed silk; 5g of degummed silk was placed in 50g of 9.3mol / L LiBr aqueous solution, Incubate at 50°C until the degummed silk is completely dissolved, put it into a dialysis bag with a cut-off molecular weight of 3500 Daltons, dialyze in distilled water with a flow rate of 1 mL / min for 5 days, and centrifuge to obtain the supernatant as silk fibroin aqueous solution. The mass fraction of silk fibroin in silk fibroin aqueous solution was determined by Coomassie brilliant blue method, diluted with distilled water until the mass fraction of silk fibroin was 6%, and stored at 5°C for later use.

[0045] 2. Prepare the scaffold

[0046]Mi...

Embodiment 2

[0048] In step 2 of preparing the scaffold in Example 1, 4 g of NaCl with a particle size of 180-250 μm and 0.08 g of hydroxyapatite are mixed evenly, and the mass ratio of NaCl to hydroxyapatite is 1:0.02. Other steps of this step Same as Example 1. The other steps were the same as in Example 1 to prepare a silk fibroin / hydroxyapatite / collagen composite porous scaffold.

Embodiment 3

[0050] In step 2 of preparing the scaffold in Example 1, 4 g of NaCl with a particle size of 180-250 μm and 0.24 g of hydroxyapatite were mixed evenly, and the mass ratio of NaCl to hydroxyapatite was 1:0.06. Other steps of this step Same as Example 1. The other steps were the same as in Example 1 to prepare a silk fibroin / hydroxyapatite / collagen composite porous scaffold.

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Abstract

A preparation method of a silk fibroin / hydroxyapatite / collagen composite porous stent comprises the following steps: filling a mixture of NaCl and hydroxyapatite into an injector, wherein the mixture has a particle size of 100-425 microns and a mass ratio of NaCl and hydroxyapatite of 1:0.02-0.08; adding a mixed solution of a collagen aqueous solution with a mass fraction of 6% and a silk fibroinaqueous solution with a mass fraction of 6%, wherein each gram of NaCl is added with 1 ml of the mixed solution; pushing the piston handle of the injector to allow the mixed solution to pass the mixture of NaCl and hydroxyapatite; allowing the mixture to stand at room temperature for 24 hours; taking out the bonded block from the injector, soaking and washing. The invention can prepare stents with various pore sizes by adjusting the particle sizes of NaCl, and the prepared stent has a uniform pore size, high porosity, good pore connectivity, moderate water absorption and expansion rate. Test results show that the stent has good in-vitro and in-vivo biocompatibility.

Description

technical field [0001] The invention belongs to the technical field of materials, and in particular relates to a preparation method of a porous support. Background technique [0002] An ideal scaffold should have the characteristics of suitable pore size, high porosity, good pore connectivity, high mechanical strength, and good biocompatibility. Nano-hydroxyapatite has high mechanical strength and its chemical properties are similar to natural bone, so it can be used as a bone substitute in the biomedical field. However, due to the high hardness, high brittleness and lack of elasticity of nano-hydroxyapatite, it is difficult to form and maintain the desired shape. Collagen is the most important extracellular matrix in the body, but it has low mechanical strength and high cost. Silk fibroin is a protein extracted from silkworm cocoons, which has the advantages of high mechanical strength, good toughness, and high elasticity. Therefore, combining silk fibroin, nano-hydroxya...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J9/26C08L89/04C08L89/00C08K3/32A61L27/46A61L27/56
Inventor 张志琪牟朝丽祁小妮段俐敏
Owner SHAANXI NORMAL UNIV
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