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Method for producing regenerated plantlet of Valeriana amurensis P.Smirn calluses

A technology for callus and regeneration of plants, applied in botany equipment and methods, plant regeneration, horticultural methods, etc., can solve the problems of long propagation cycle, scarcity of blackwater valerian, low proliferation efficiency, etc., and achieve short cultivation cycle , Conducive to large-scale production, good effect of genetic stability

Inactive Publication Date: 2012-01-04
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a method for regenerating plants from blackwater valerian callus in order to solve the problems of blackwater valerian infrequent quantity, long propagation cycle and low propagation efficiency

Method used

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  • Method for producing regenerated plantlet of Valeriana amurensis P.Smirn calluses
  • Method for producing regenerated plantlet of Valeriana amurensis P.Smirn calluses
  • Method for producing regenerated plantlet of Valeriana amurensis P.Smirn calluses

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specific Embodiment approach 1

[0014] The specific embodiment one: the method for plant regeneration of blackwater valerian root callus of the present embodiment is realized by the following steps: one, the black water valerian root seed is sterilized after freezing pretreatment, then inoculates into 1 / 2 of the addition of 7g / L agar On 2MS medium, cultivate for 30-37 days under the conditions of temperature 24-26°C, humidity 60%-80%, light intensity 3000 lux, light 16h per day, to obtain seedlings;

[0015] 2. Inoculate the seedlings with a length of 2 cm on MS medium supplemented with 7 g / L agar, and cultivate them under the condition that the temperature is 24-26 °C, the humidity is 60%-80%, the light intensity is 3000 lux, and the light is 16 hours per day. ~3 months, get sterile vaccine;

[0016] 3. Take the new young leaves of sterile seedlings and cut them into discs with a diameter of 1cm, and then inoculate them into MS with 2.0mg / L2, 4-D, 0.15mg / L kinetin, 30g / L sucrose and 7g / L agar. On the cultu...

specific Embodiment approach 2

[0025] Specific embodiment 2: The difference between this embodiment and specific embodiment 1 is that the process of freezing pretreatment in step 1 is: rinse the healthy and plump seeds 5 times with tap water, absorb the surface moisture with filter paper, and then place them in a petri dish , stored at -4°C for 1 week. Other steps and parameters are the same as those in Embodiment 1.

[0026] The purpose of freezing pretreatment in this embodiment is to break seed dormancy.

specific Embodiment approach 3

[0027] Specific embodiment three: The difference between this embodiment and specific embodiment one is that the process of disinfection in step one is: soak the frozen pretreated seeds overnight with sterile water at room temperature, rinse them with sterile water in a sterile table Wash 5 times, then dry the surface moisture with sterilized filter paper, wrap the seeds in a small piece of sterile gauze, soak in ethanol with a volume concentration of 75% for 1 min, then wash 2 times with sterile water, and then place At a mass concentration of 0.1% HgCl 2 soak for 5 minutes, then wash with sterile water for 5 times, and dry the surface moisture with sterilized filter paper. Other steps and parameters are the same as those in Embodiment 1.

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Abstract

The invention provides a method for producing a regenerated plantlet of Valeriana amurensis P.Smirn calluses and relates to a method for producing a regenerated plantlet of Valeriana amurensis P.Smirn. The method provided by the invention solves the problems that the number of the Valeriana amurensis P.Smirn is less, the breeding cycle is long and the proliferation efficiency is low. The method comprises the following steps of: (1), processing seeds of the Valeriana amurensis P.Smirn and then culturing the seeds to obtain seedlings; (2), culturing the seedlings to obtain sterile seedlings; (3) cutting off new tender leaves of the sterile seedlings and culturing the leaves to obtain calluses; then carrying out a successive transfer culture to obtain adventitious buds; (4), cutting off the adventitious buds with the calluses on the base part and culturing to obtain plantlets; and (5), cutting off the plantlets from the base part and immersing the plantlets in kinetin; and then culturingto form complete plants. The method provided by the invention has the advantages of simple formulation, simple operation process, short culture cycle, great proliferation coefficient (7.5-9.5 times are propagated every one month), high regeneration frequency and low cost; and the method is good for production in a large scale.

Description

technical field [0001] The invention relates to a method for regenerating plants with black water valerian. Background technique [0002] Blackwater valerian (V.amurensis Smir.ex Kom.) is a wild perennial herb of Valerianaceae (Valerianaceae). Valerian has a wide range of pharmacological activities and a long history of medication. Its nature is flat and spicy, and it has the effects of calming the nerves, relieving spasms and relieving pain. Its rhizome has been used as a sedative in the folk for a long time. There are records. In northwestern Europe, valerian, which consists of the underground part of valerian, has been used as a sedative for a long time. The ancient Greeks and Romans had already identified valerian and used it as a mild sedative. According to incomplete statistics, there are currently 81 kinds of valerian in 25 countries in the world that have been studied to varying degrees, among which V.officiolis.L has been included in the Pharmacopoeia of Germany, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 王振宇崔磊周秀华赵海田
Owner HARBIN INST OF TECH
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