Method for preparing oligopeptide of snake meat

A technology of oligopeptide and snake meat, applied in food preparation, hydrolyzed protein components, food science, etc., can solve the problems of low activity, poor solubility, low absorption rate, etc.

Active Publication Date: 2014-02-19
唐作安
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For snake meat, a high-protein food, the physical method of drying and crushing cannot fundamentally change the properties of protein macromolecules, and the product has low activity, low absorption rate, and poor solubility, so the application of the product is severely limited.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0008] Select 10Kg of adult healthy living bodies of black snake, Agkistrodon halys, cobra, Agkistrodon halys, and Chinese water snake, remove the head, tail, skin and viscera after slaughtering, wash the carcass with clean water; mince with a meat grinder, The degree of mincing is controlled by a φ8mm orifice plate; the snake meat is refined to a fineness of 20-30 μm with an ultramicro grinder; the snake meat slurry is pumped into the reaction kettle, the substrate concentration is adjusted to 15% with pure water, and heated to 50 ℃, add Protamex compound protease marked with an activity of 1.5AU / g, the amount of enzyme added is 0.4kg, stir evenly, without adjusting the pH value of the material, the duration of enzymolysis is 6 hours; filter with a 100-mesh stainless steel mesh filter, pressure 0.4 Mpa; the enzymolysis solution is heated to 90°C and kept for 10 minutes to inactivate the enzyme; desalination, arsenic removal, and concentration treatment with a nanofiltration me...

Embodiment 2

[0010] Select 5Kg of adult healthy living bodies of red chain snake, coral snake, Agkistrodon sharp kiss, brown black snake, Chifeng golden snake, and rock-dwelling snake. After slaughtering, remove the head, tail, skin and internal organs, and wash the carcass with clean water. Wash it; grind it with a meat grinder, and the degree of grinding is controlled by a φ10mm orifice plate; use an ultramicro grinder to refine the snake meat to a fineness of 30-50 μm; pump the snake meat slurry into the reaction kettle, and adjust the bottom with pure water to 10% concentration, heated to 55°C, added Protamex complex protease marked with an activity of 1.5AU / g, added 0.3kg of enzyme, stirred evenly, no need to adjust the pH value of the material, and the duration of enzymolysis was 8 hours; Filtrate with a 120-mesh stainless steel mesh filter with a pressure of 0.3Mpa; heat the enzymolysis solution to 85°C and keep it for 15 minutes to inactivate the enzyme; use a nanofiltration membran...

Embodiment 3

[0012] By embodiment 1 or embodiment 2 gained snake meat oligopeptide powder, do not add other any composition, make snake meat oligopeptide hard capsule product with capsule filling machine. You can also use 50-70% snake meat oligopeptide powder, add red ginseng, Ophiopogon japonicus, wolfberry, Eucommia, yam, angelica, Codonopsis pilosula, astragalus, salvia miltiorrhiza, Panax notoginseng, Chuanxiong, kudzu root, Schisandra chinensis, Acanthopanax, Epimedium Huo, Cuscuta, Cistanche, saffron and other traditional Chinese medicine extracts 30-50%, made into hard capsule products with different functions.

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PUM

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Abstract

The invention discloses a method for preparing oligopeptide of snake meat, which comprises the following steps of: selecting a healthy and live adult snake from zaocys dhumnade, hagworm, gloydius saxatilis, dinodon rufozonatum, cobra, coral snakes, agkistrodon acutus, elaphe schrenckii, elaphe anomala, enhydris chinensis, and the like, slaughtering, removing the head, tail, skin and viscera of the snake, cleaning a carcass part with clear water, and performing processes such as grinding, jordaning, enzymolysis, filtration, enzyme deactivating, salt and arsenic removal, nanofiltration and concentration, spray drying and the like to obtain dry oligopeptide powder of the snake meat. The obtained product has the molecular weight of over 85 percent and the molecular weight distribution of 200-5,000Dalt; and bioactive substances have the effects of resisting tumors, coagulation, oxidation and fatigue, improving immunity, preventing heart cerebrovascular disease, preventing and treating rheumatic arthritis and the like.

Description

technical field [0001] The invention relates to a method for processing and preparing snake meat oligopeptides by using protein enzymatic hydrolysis technology and a method for making functional food by using snake meat oligopeptides. It belongs to the field of functional food. Background technique [0002] Snake is an animal with high nutritional value for both medicine and food. According to the "Compendium of Materia Medica": "Snakes enter the liver and spleen meridians, dispel rheumatism, disperse sores, dispel wind and attack poison, treat ringworm and itchy body, go through the viscera inside, and penetrate the skin outside." Modern medical research has proved that snake body contains 18 kinds of amino acids (among them, 8 kinds of amino acids that cannot be synthesized by the human body and are necessary for a large proportion), and the amino acid distribution of snake meat protein is very close to that of human protein. It is widely used among folks to strengthen t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06A61K38/01A61P35/00A61P7/02A61P39/06A61P39/00A61P37/04A61P9/00A61P19/02A61P29/00A23L1/305A23L1/29A23L33/00
Inventor 唐作安李采东
Owner 唐作安
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