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A method for regulating the fermentation process of fumaric acid-producing Rhizopus

A fermentation process, fumarate technology, applied in the field of bioengineering to achieve high efficiency

Inactive Publication Date: 2011-12-21
NANJING TECH UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

For Rhizopus, there is no report on the regulation of its biosynthetic fumaric acid fermentation process based on the analysis of metabolomics technology.

Method used

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  • A method for regulating the fermentation process of fumaric acid-producing Rhizopus
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Embodiment 1

[0030] This embodiment illustrates that fumarate-producing Rhizopus oryzae ( Rhizopus oryzae ) method for regulating the fermentation process.

[0031] Strains: (1) The original strain Rhizopus oryzae (ATCC 20344).

[0032] (2) High-yield fumaric acid mutant strains: the above-mentioned original strains were treated with a femtosecond laser with a wavelength of 800 nm and a repetition rate of 76 MHz under the conditions of an irradiation power of 5-40 mW and an irradiation time of 5-30 s. treatment, obtained a high-yield fumaric acid strain of Rhizopus oryzae; the optimized femtosecond laser mutagenesis method used therein comes from the patent publication number CN102061294A, and the name is "Femtosecond laser mutagenesis of Rhizopus oryzae for breeding fumaric acid high-yield strains method” patent application literature.

[0033] 1. Synchronously culture the above-mentioned original strain and the high-yielding mutant strain induced by femtosecond laser mutagenesis ...

Embodiment 2

[0039] This embodiment illustrates that fumaric acid-producing Rhizopus aureus ( Rhizopus arrhizus ) method for regulating the fermentation process.

[0040] Strains: (1) The original strain Rhizopus arrhizus (NRRL 2582).

[0041] (2) High-yield mutant strain of fumaric acid: the above-mentioned original strain was treated with a UV lamp with a power of 15 W, an irradiation distance of 30 cm, and an irradiation time of 50 s, and a strain of Rhizopus lessi with high yield and rich malic acid strain.

[0042] 1. Synchronously culture the high-yield mutant strain of Rhizopus alicifera and the original bacterial strain at 400 rpm, 35° C. of temperature, 10% inoculum size, and a 7 LNBS fermenter with a liquid filling capacity of 5 L (the composition of the fermentation medium and the mass content For: glucose 80 g, (NH 4 ) 2 SO 2 2 g, yeast extract (total nitrogen content 9.8%) 0.4 g, KH 2 PO 4 0.4 g, MgSO 4 ·7H 2 O 0.4 g, ZnSO 4 7H2O 0.05 g, FeSO 4 ·7H 2 O 0.01...

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Abstract

The invention belongs to the field of biological engineering and relates to a method for controlling the fermentation process of fumaric acid producing rhizopus oryzae. In the invention, through the measurement and comparative analysis of the metabolome of a high-yield rhizopus oryzae strain and the metabolome of an original strain, high-yield fumaric acid intracellular metabolism characteristic information of rhizopus oryzae is acquired completely to provide an important guide for the control and optimization of the fermentation process. Compared with other fermentation control policies, the method can perform detrended analysis on all metabolites in cells, help to get deeper understanding of the fumaric acid high yield mechanism of the, and is more specific and efficient for fermentation control; and therefore the method is a high-efficiency and systemic method for control and optimization of the fermentation process.

Description

technical field [0001] The invention belongs to the field of bioengineering, and relates to a method for regulating the fermentation process of fumarate-producing Rhizopus, in particular to a method for regulating the biosynthesis of fumaric acid by Rhizopus based on metabolomics technology. Background technique: [0002] Fumaric acid is a dibasic carboxylic acid containing double bonds. It can produce L-aspartic acid, malic acid, succinic acid and 1,4-butanediol through ammoniation, hydration, hydrogenation and isomerization. , maleic acid and other compounds, at the same time, as important organic chemical raw materials and fine chemical products are also widely used in the fields of materials (resin, coating and plasticizer, etc.), medicine, chemical industry, food and feed additives (Li Xuekun, Zhang Kun, Gao Zhen, etc. Synthesis and application of fumaric acid [J]. Modern Chemical Industry, 2005, 25(suppl1): 81-84.). At present, fumaric acid is mainly converted from p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/46G01N27/62C12R1/845
Inventor 黄和李霜闻建平于守智徐晴
Owner NANJING TECH UNIV
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