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Method for expanding culture of microorganisms for wastewater treatment and microbial wastewater treatment method

A technology for wastewater treatment and expanded cultivation, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as difficult to achieve effects, death, and difficult domestication of strains, and achieve the effect of improving efficiency and efficient degradation

Active Publication Date: 2011-12-07
北京赛富威环境工程技术有限公司
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Problems solved by technology

[0005] However, in the practical application of wastewater treatment plants using the activated sludge method, because the content of different organic substances in wastewater is difficult to be consistent for a long time, and such organic substances have certain biological toxicity, therefore, it is often difficult for activated sludge to be used under various conditions. This will lead to the following problems: the COD of the wastewater has little or no change; for different types of wastewater, or even for the same type of different batches of wastewater, it is necessary to replace the active Sludge; unstable sludge efficiency; insufficient number of effective strains; and mass death of the strains
[0007] However, the inventors of the present application have found that there are the following problems in the treatment of waste water by bioaugmentation technology, especially the treatment of waste water containing heterocyclic compounds, polycyclic aromatic hydrocarbons, cellulose and lignin: (1) if the special bacteria are directly added to the waste water Among them, it takes a long time for special strains that have not been pre-acclimated to reach the logarithmic growth phase, thus reducing the efficiency of sewage treatment; (2) If there are substances that inhibit the growth of strains in the system, even after experiencing For a long time, it will also cause the bacterial classification to be difficult to domesticate or die, and it is difficult to achieve the purification effect; (3) in the prior art, the above several types of wastewater need to use different special bacterial classifications and different treatment conditions, which is necessary for daily use. It is extremely inconvenient for waste water treatment plants that all need to face various waste water sources; and (4) if only various special strains for different waste water are simply mixed, growth inhibition, etc. will occur between the strains phenomenon, it is difficult to achieve the desired effect

Method used

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  • Method for expanding culture of microorganisms for wastewater treatment and microbial wastewater treatment method
  • Method for expanding culture of microorganisms for wastewater treatment and microbial wastewater treatment method
  • Method for expanding culture of microorganisms for wastewater treatment and microbial wastewater treatment method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Embodiment 1 uses the method of the present invention to domesticate the effect of the microorganism of amplification to carry out wastewater treatment

[0053] 1.1 Experimental objects:

[0054] Wastewater from a coking plant. After testing, the wastewater that entered contained pollutants such as phenol, xylenol, quinoline, isoquinoline, indole, and pyridine.

[0055] 1.2 Experimental raw materials:

[0056] The compound microbial agent is 250ppm of Pseudomonas putida, 250ppm of Pseudomonas fluorescens, 250ppm of Bacillus subtilis, 250ppm of Bacillus thuringiensis and 500ppm of white rot fungus. The enzyme preparation is 500ppm of protease, 500ppm of amylase, 500ppm of cellulase and 500ppm of lipase. Citric acid is 250ppm. Riboflavin is 250ppm. The dosage unit of all additives is ppm, which refers to the corresponding concentration of the substance after it is added to the target wastewater biological treatment system.

[0057] 200L effective volume domesticat...

Embodiment 2

[0068] Embodiment 2 uses the microorganism of the inventive method domestication amplification to carry out the effect of wastewater treatment

[0069] 1.1 Experimental object: Antibiotic wastewater from a pharmaceutical factory. After testing, the wastewater entering the treatment station contains quinolines, pyridines, oxazoles, diazoles, triazoles, carbazoles, triazines and other pollutants.

[0070] 1.2 Experimental raw materials:

[0071] The compound bacterial agent is Pseudomonas fluorescens 5×10 4 ppm, Bacillus thuringiensis 5×10 4 ppm and white rot fungi 5×10 4 ppm. Enzyme preparation is protease 5×10 4 ppm, Amylase 5×10 4 ppm, Cellulase 5×10 4 ppm and lipase 5×10 4 ppm. Citric acid is 1 x 10 5 ppm. Riboflavin is 2.5 x 10 4 ppm. The dosage unit of all additives is ppm, which refers to the corresponding concentration of the substance after it is added to the target wastewater biological treatment system.

[0072] 200L effective volume domestication react...

Embodiment 3

[0084] Embodiment 3 uses the method of the present invention to domesticate the effect of the microorganism that amplifies carries out wastewater treatment

[0085] 1.1 Experimental object: Wastewater from printing and dyeing factories. After testing, the wastewater entering the treatment station contains quinone, anthracene, phenanthrene, perylene, azo, biphenyl, and naphthalene pollutants.

[0086] 1.2 Experimental raw materials:

[0087] The compound bacterial agent is Pseudomonas putida 2.5×10 4 ppm, Pseudomonas fluorescens 2.5×10 4 ppm, Bacillus subtilis 2.5×10 4 ppm, Bacillus thuringiensis 2.5×10 4 ppm and white rot fungus 2.5×10 4 ppm. Enzyme preparation is protease 5×10 4 ppm, Amylase 5×10 4 ppm, Cellulase 5×10 4 ppm and lipase 5×10 4 ppm. Citric acid is 1 x 10 5 ppm. Riboflavin is 2.5 x 10 4 ppm. The dosage unit of all additives is ppm, which refers to the corresponding concentration of the substance after it is added to the target wastewater biologica...

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Abstract

A method of amplification culture of microorganism for waste water treatment. The waste water comprises heterocyclic compounds, polycyclic aromatic hydrocarbons, cellulose and / or lignin. The method comprises the following steps: adding waste water containing heterocyclic compounds, polycyclic aromatic hydrocarbons, cellulose and / or lignin into an acclimation reactor; adding a nutritional agent, acomplex bacterial agent, an enzyme preparation, a citric acid and riboflavin on a basis of concentrations in the waste water; performing aeration of the waste water, adjusting the liquid level and temperature of the acclimation reactor, holding for a period of time to allow the microorganism in the complex bacterial agent to reach a logarithmic growth phase, wherein on a basis of concentrations in the waste water, the complex bacterial agent comprises pseudomonas spp. microorganism, bacillus sp. microorganism, and white rot fungi, and the enzyme preparation comprises protease, amylase, cellulose and lipase. The invention also provides a method of waste water treatment by using the microorganism to be amplified and cultured.

Description

technical field [0001] The invention relates to a method for expanding the cultivation of microorganisms for wastewater treatment and a method for using the expanded cultivation of microorganisms for wastewater treatment, in particular to a method for expanding the cultivation of microorganisms containing heterocyclic compounds, polycyclic aromatic hydrocarbons, cellulose and / or lignin A method for microorganisms of wastewater, and a method for treating wastewater using the microorganisms. Background technique [0002] Heterocyclic compounds (heterocyclic compounds, HCCs), polycyclic aromatic hydrocarbons (polycyclic aromatic hydrocarbons, PAHs), lignin and cellulose exist in large quantities in chemical, pharmaceutical, printing and dyeing, and papermaking wastewater, and they are the most common in wastewater treatment plants at present. Pollutants, if not treated, such pollutants can cause serious pollution to the environment. Methods for treating such wastewater include...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/14C02F3/34C12R1/38C12R1/39C12R1/40C12R1/07C12R1/125C12R1/10C12R1/085C12R1/11C12R1/645C12R1/20C12R1/05C12R1/13C12R1/01C02F101/30C02F101/32C02F101/34
Inventor 温捷王野
Owner 北京赛富威环境工程技术有限公司
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