Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of Jinpine Biflavone in the Preparation of Drugs and Foods for Preventing and Treating Osteoporosis

A technology of Jinsongbiflavonoids and osteoporosis, which is applied in the field of medicine, can solve the problems that have not been reported in the literature and the like, achieve huge social and economic benefits, improve the density of femur and femur, and is easy to prepare.

Inactive Publication Date: 2011-11-30
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
View PDF1 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The role and efficacy of Jinsongbiflavonoids in the prevention and treatment of osteoporosis have not been reported in the literature

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of Jinpine Biflavone in the Preparation of Drugs and Foods for Preventing and Treating Osteoporosis
  • Application of Jinpine Biflavone in the Preparation of Drugs and Foods for Preventing and Treating Osteoporosis
  • Application of Jinpine Biflavone in the Preparation of Drugs and Foods for Preventing and Treating Osteoporosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1 (preparation of golden pine bisflavone)

[0017] Collect 5 kg of dried Chinese fir branches and leaves, crush them, and extract them ultrasonically for 3 times with 95% ethanol. The extracts are combined, concentrated under reduced pressure, degreased by petroleum ether ultrasonically, then extracted with ethyl acetate, filtered, and the ethyl acetate extracts are decompressed and concentrated , dried to obtain the crude extract, the ethyl acetate crude extract was subjected to silica gel column chromatography 1400g (particle size 200-300 mesh, 10cm×20cm), petroleum ether-ethyl acetate (1:9~1:5) gradient elution , tracked and detected by TLC, combined, the eighth fraction was decompressed, concentrated to dryness, and rinsed with methanol to remove impurities. After vacuum drying, the golden pine bisflavone yellow powder was obtained, which was detected by HPLC with a purity greater than 95%, and was used for subsequent in vivo and in vitro pharmacological ...

Embodiment 2

[0018] Embodiment 2 (the effect of pine bisflavone on osteosarcoma cell MG-63-PERE)

[0019] Cells: Osteogenic sarcoma cells MG-63-PERE stably transfected with the estrogen response element as the promoter and firefly luciferase as the reporter gene provided by the School of Pharmacy, Second Military Medical University of the Chinese People's Liberation Army.

[0020] Experimental process: plate 5000 cells per well on a 96-well plate, add medicine (DMSO concentration < 2%) after the cells adhere to the wall, remove the supernatant after culturing in the incubator for 6 hours, wash with ice-cold PBS, add 20 μl of cell lysate is ready for use.

[0021] Detection method: measure on a chemiluminescence detector, use a dual reporter gene detection system, first measure the luminescence of firefly luciferase, and then quench to measure the luminescence of the internal standard Renilla luciferase.

[0022] Experimental results: the results are represented by the percentage of the am...

Embodiment 3

[0027] Embodiment 3 (prevention and treatment of rat osteoporosis caused by diflavonoids to retinoic acid)

[0028] 1. Experimental materials

[0029] 1.1. Animals

[0030] SD rats were provided by the Experimental Animal Center of the Second Military Medical University of the Chinese People's Liberation Army.

[0031] 1.2. Drugs

[0032] Sodium hydroxyethylphosphonate, provided by the New Drug Evaluation Center of the School of Pharmacy, Second Military Medical University of the Chinese People's Liberation Army, made 2.0% suspension with 0.5% CMC-Na;

[0033] Retinoic acid, Shanghai Sixth Pharmaceutical Factory, batch number 200106012, made 1.5% suspension with 0.5% CMC-Na;

[0034] Jinpine biflavone, prepared by Example 1, is made into a suspension with 0.5% CMC-Na.

[0035] CMC-Na, product of Sinopharm Shanghai Chemical Reagent Co., Ltd.

[0036] 1.3. Instruments

[0037] QCT-bone density analysis system, American LACA company.

[0038] 2. Methods and results

[003...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the application of the biflavone of golden pine in the preparation of medicine and food for preventing and treating osteoporosis. Pharmacological studies in vitro and in vivo have shown that amiflavonoids have estrogen-like effects and can significantly increase femur stock density, bone ash weight, bone calcium content and bone phosphorus content in rats with osteoporosis caused by retinoic acid, and have significant prevention and treatment of osteoporosis. role, and has application prospects in the preparation of drugs for the prevention and treatment of osteoporosis.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a new medical application of aquinone. Background technique [0002] Osteoporosis is a metabolic bone disease characterized by decreased bone mass and destruction of bone microarchitecture, leading to increased bone fragility and susceptibility to fractures. The disease mainly occurs in the elderly and postmenopausal women. With the arrival of an aging society, the incidence of this disease is increasing year by year. There are about 90 million osteoporosis patients in my country. Anti-osteoporosis drugs have a very broad market prospect. The research and development of indication drugs is one of the hot spots in the research and development of new drugs. [0003] Existing studies have shown that the pathogenesis of osteoporosis lies in the process of bone formation, the main function of osteoclasts is to remove damaged debris and form bone holes, and the main role of osteoblast...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/352A61P19/10A23L1/30
Inventor 张磊辛海量王玉亮唐克轩唐罗欧龙娉王卓
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com