Cordyceps cicadae wine and preparation method thereof
A technology of cicadae and flower wine, which is applied in the preparation of alcoholic beverages, biochemical equipment and methods, medical preparations containing active ingredients, etc., and can solve the problems of inability to apply cicadae wine to mass production, high price of cicadae, and scarcity of output, etc. problem, to achieve the effect of improving effective utilization rate, stable quality and reducing production cost
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Embodiment 1
[0070] The acquisition of embodiment 1 Paecilomyces cicadae fermentation product
[0071] 1. Preparation and cultivation of strains:
[0072] (1) Strain preparation: prepare potato sucrose agar medium or potato sucrose culture solution for subsequent use;
[0073] ①Preparation of slant seeds: wild cicadas collected from the source of Sanjiang in Yunnan Province were obtained through tissue separation and purification, and after transferring to tubes, they were cultured at 22°C for 5 days, and selected for future use;
[0074] ②Preparation of the primary seed: put the potato sucrose culture solution into the Erlenmeyer flask, put it in a sterilizing pot and sterilize it under the pressure of 0.15MPa for 30min, and put the spare slanted seed into the Erlenmeyer flask under aseptic condition after cooling , cultivated for 5 days at 22°C with an oscillation frequency of 130r / min, until a large number of mycelium balls appeared in the Erlenmeyer flask and then used for later use; ...
Embodiment 2
[0084] The acquisition of embodiment 2 Paecilomyces cicadae fermentation product
[0085] 1. Preparation and cultivation of strains:
[0086] (1) Strain preparation: prepare potato sucrose agar medium or potato sucrose culture solution for subsequent use;
[0087] ①Preparation of slant seeds: wild cicadae collected from the source of Sanjiang in Yunnan Province was obtained through tissue separation and purification, and after transferring to tubes, cultured at 25°C for 7 days, and selected for future use;
[0088] ②Preparation of the primary species: put the potato sucrose culture solution into a conical flask, sterilize it in a sterilizing pot for 30 minutes under a pressure of 0.15MPa, put it into a conical flask after cooling, and place it at 25°C with an oscillation frequency of 150r / Cultivate for 3 days under the condition of min, until a large number of mycelium balls appear in the Erlenmeyer flask for later use;
[0089] Potato sucrose agar medium and potato sucrose...
Embodiment 3
[0094] The acquisition of embodiment 3 Paecilomyces cicadae fermentation product
[0095] 1. Preparation and cultivation of strains:
[0096] (1) Strain preparation: prepare potato sucrose agar medium or potato sucrose culture solution for subsequent use;
[0097] ①Preparation of slant seeds: wild cicadas collected from the source of Sanjiang in Yunnan Province were obtained through tissue separation and purification, and after transferring to tubes, they were cultured at 22°C for 5 days, and selected for future use;
[0098] ②Preparation of the primary species: put the potato sucrose culture solution into a conical flask, put it in a sterilizer for 30 minutes under a pressure of 0.15 MPa, put it into a conical flask after cooling, and place it at 23°C with an oscillation frequency of 130r / Cultivate under the condition of 5 min for 5 days, until a large number of mycelium balls appear in the Erlenmeyer flask for subsequent use;
[0099] Potato sucrose agar medium and potato s...
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