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Preparation for reconstruction influenza A H1N1 virus inactivated vaccine strain (SC/PR8), and use thereof

A technology of influenza virus and inactivated vaccines, which is applied in the direction of viruses/bacteriophages, antiviral agents, and medical preparations containing active ingredients, etc., which can solve the problems of short immunization period, difficulty, and high cost of vaccines

Inactive Publication Date: 2011-11-09
HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is difficult to isolate strains with the above conditions from nature as vaccine strains
In China, due to the poor adaptability of chicken embryos isolated from wild strains, the current domestic H1N1 influenza vaccines have disadvantages such as high cost and short vaccine immunity period.

Method used

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  • Preparation for reconstruction influenza A H1N1 virus inactivated vaccine strain (SC/PR8), and use thereof
  • Preparation for reconstruction influenza A H1N1 virus inactivated vaccine strain (SC/PR8), and use thereof
  • Preparation for reconstruction influenza A H1N1 virus inactivated vaccine strain (SC/PR8), and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Embodiment 1: Screening, establishment of identification recombinant virus method

[0059] Using the TrIzol strong fission agent method, extract the RNA of influenza virus A / PR / 8 / 34 (H1N1) and influenza A H1N1 influenza isolate A / SC / 1 / 2009 (H1N1), carry out reverse transcription, and then use the above-mentioned design The fragment-specific identification primers of influenza virus A / PR / 8 / 34 (H1N1) and influenza A H1N1 influenza isolate A / SC / 1 / 2009 (H1N1) were used for PCR reaction. The fragment-specific identification primers designed according to the gene of influenza virus A / PR / 8 / 34 (H1N1) can only amplify 8 specific fragments of influenza virus A / PR / 8 / 34 (H1N1) strain but cannot amplify Add any fragments of the A / SC / 1 / 2009 (H1N1) strain of the A / SC / 1 / 2009 (H1N1) influenza strain, and fragment-specific identification primers designed according to the gene of the A / SC / 1 / 2009 (H1N1) influenza A / H1N1 influenza isolate Only 8 specific fragments of influenza A / SC / 1 / 2009 ...

Embodiment 2

[0063] Example 2: Preparation and Identification of Recombinant Influenza A H1N1 Virus

[0064] 1. Co-infection - Recombination of two viral genomes:

[0065] 100 μl of influenza A H1N1 domestic isolate strain A / SC / 1 / 2009 (H1N1) and 50 μl of influenza virus A / PR / 8 / 34 (H1N1) (PR8 for short) were mixed and added to 850 μl of PBS, and co-inoculated at 10 days old The allantoic cavity of SPF chicken embryos undergoes natural reorganization. After culturing at 35°C for 24 hours, the allantoic fluid was collected.

[0066] 2. Antibody screening-removal of strains whose surface genes are derived from influenza virus A / PR / 8 / 34 (H1N1):

[0067] Take 50 μl of the allantoic fluid harvested under the above-mentioned specific artificial conditions and mix with 600 μl of anti-A / PR / 8 / 34 (H1N1) antiserum diluted by 4 times, react at room temperature for 30 minutes, and then inoculate each dilution Three chicken embryos. Next, the inoculated chicken embryos were cultured in a 35°C incubato...

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PUM

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Abstract

The invention relates to a reconstruction influenza A H1N1 virus inactivated vaccine strain. The reconstruction influenza A H1N1 virus inactivated vaccine strain is characterized by expressing HA protein and NA protein of the influenza A H1N1 virus. In particular, the reconstruction influenza A H1N1 virus inactivated vaccine strain is SC / PR8. The invention further discloses a method for preparing the reconstruction influenza A H1N1 virus inactivated vaccine strain (SC / PR8), and a use of the reconstruction influenza A H1N1 virus inactivated vaccine strain (SC / PR8) in prevention of animal influenza A H1N1 or human influenza A H1N1.

Description

technical field [0001] The present invention relates to a recombinant genetic engineering vaccine, specifically, the present invention relates to a recombinant genetic engineering vaccine for preventing animal and human type A H1N1 influenza, more specifically, the vaccine involved in the present invention is a vaccine for preventing animal and human Recombinant influenza A H1N1 virus inactivated vaccine for human influenza A H1N1. Background technique [0002] Influenza A virus is an enveloped virus composed of segmented negative-sense RNA that can widely infect birds and mammals. In the world, influenza often occurs in the form of sporadic outbreaks and random pandemics. In the past few decades, the main choice of various countries is still the whole virus inactivated vaccine, which mainly stimulates the body to produce humoral immune resistance. Subtype of the hemagglutinin surface glycoprotein. [0003] As of December 2009, the H1N1 influenza pandemic that started in M...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/04C12N7/01A61K39/145A61P31/16C12R1/93
Inventor 陈化兰施建忠步志高邓国华姜永萍田国彬李雁冰乔传玲
Owner HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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