Rotor-type internal-compression oil-gas mixed transport pump unit
A diketone and diene technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of few reports worldwide
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[0041] The preparation method of the steroid compound intermediate 11,17α-substituted pregna-1,4-diene-3,20-dione of the present invention uses microorganisms to prepare the 11,17α-substituted pregna-4- Conversion of ene-3,20-dione (intermediate A) to 11,17α-substituted pregna-1,4-diene-3,20-dione (intermediate B); the microorganism can adopt Arthrobacter simplex ( Arthrobacter Simplex, ATCC 6946 ); including the following steps:
[0042] The first step is to configure and process the culture medium;
[0043] The culture medium is configured according to the following proportions:
[0044] The culture medium includes nitrogen-containing substances, inorganic salts, water, 0.5-1.5% glucose (percentage by weight), and 0.5-1.5% corn steep liquor (percentage by weight);
[0045] Nitrogen-containing substances can be added with bean cake powder, peptone, yeast extract, yeast extract, beef extract, etc.; the addition amount is 0.2-0.5% (weight percentage);
[0046] Inorganic sal...
Embodiment 1
[0066] A 500ml flask was filled with 200ml of medium containing 1% (weight percent) glucose, 1% (weight percent) corn steep liquor, 0.3% (weight percent) peptone, 0.15% (weight percent) KH 2 PO 4 ; Sterilize the culture medium in an autoclave at 120°C for 30 minutes; cool it to 30±1°C, then inoculate the slant culture of Arthrobacter simplex in the culture medium; place the culture medium inoculated with the strain in a shaker Cultivate at 165rpm at 30±1°C for 24 hours;
[0067] 10.0 g of 11α, 17α-dihydroxy-pregn-4-ene-3,20-dione was pulverized, sterilized by dry heat, and then put into the culture medium; continued to stir and ventilate; control the pH to 5.4; keep the fermentation temperature at 30±1℃;
[0068] After 48 hours, the culture solution was harvested, and the solid matter was filtered and dried;
[0069] Decolorization and purification by conventional methods gave 6.21 g of 11α, 17α-dihydroxy-pregna-1,4-diene-3,20-dione, with a yield of 62.1% and a content of 9...
Embodiment 2
[0072] A 500ml flask was filled with 200ml of medium containing 1% (weight percent) glucose, 1% (weight percent) corn steep liquor, 0.3% (weight percent) peptone, 0.15% (weight percent) KH 2 PO 4 ; Sterilize the culture medium in an autoclave at 120°C for 30 minutes; cool it to 30±1°C, then inoculate the slant culture of Arthrobacter simplex in the culture medium; place the culture medium inoculated with the strain in a shaker Cultivate at 165rpm at 30±1°C for 24 hours;
[0073] 10.0 g of 11α, 17α-dihydroxy-pregn-4-ene-3,20-dione was pulverized, sterilized by dry heat, and then put into the culture medium; continued to stir and ventilate; control the pH to 7.8; keep the fermentation temperature at 30±1℃;
[0074] After 48 hours, the culture solution was harvested, and the solid matter was filtered and dried;
[0075] Decolorization and purification by conventional methods gave 6.32 g of 11α, 17α-dihydroxy-pregna-1,4-diene-3,20-dione, with a yield of 63.2% and a content of 9...
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