Short peptide having kidney protection function as well as preparation method and application thereof

A kidney protection and effect technology is applied to short peptides with kidney protection effect and the fields of preparation and application thereof, which can solve the problems of promoting tumor formation, increasing adverse reactions, and promoting thrombosis, and achieves easy operation, high preparation efficiency, biological Highly active effect

Inactive Publication Date: 2011-09-14
ZHONGSHAN HOSPITAL FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although EPO shows a good protective effect on renal tissue, it also has adverse reactions such as increasing blood viscosity, promoting thrombosis, promoting tumor formation and causing hypertension in clinical treatment

Method used

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  • Short peptide having kidney protection function as well as preparation method and application thereof
  • Short peptide having kidney protection function as well as preparation method and application thereof
  • Short peptide having kidney protection function as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1: Preparation of Helix B surface peptide (HBSP):

[0031] HBSP is a short peptide with renal protection. Its sequence and structure are derived from erythropoietin (EPO) B-helix surface peptide (Helix B surface peptide, HBSP). The specific amino acid sequence is QEQLERALNSS. Such as figure 1 Shown, for EPO and (EPOR) 2 Binding spatial conformation diagram, the four subunit helices of EPO (Helix A-D) form a compressed spherical structure through hydrophobic interactions, and 1 and 2 marked by dotted lines (square dotted line boxes) are the binding parts of EPO and high-affinity receptors , while the hydrophilic Helix B is located on top of the EPO spheroids (circular dashed box). Such as figure 2 Shown is a schematic diagram of the HBSP sequence, and 11 amino acid residues are extracted from the hydrophilic surface of the Helix B helix to form HBSP. Glutamate at the N-terminus can spontaneously cyclize to pyroglutamate, forming pHBSP (peptide chain shown at...

Embodiment 2

[0050] Example 2: Effect of HBSP on renal function after renal ischemia-reperfusion injury (ischemia reperfusion, IR) in rats:

[0051] a. Establish a model of renal ischemia-reperfusion injury in rats: inject ketamine solution (purchased from the Experimental Animal Center of Shanghai Zhongshan Hospital, specifications: 2ml: 0.1g) intraperitoneally at a dose of 0.12g / kg, and fix it on a plate after anesthesia. The abdominal wall was incised at the midline to enter the abdominal cavity, and the right kidney and renal pedicle were carefully separated to expose the renal pedicle. The renal pedicle was clamped with non-injured vascular clips, and the left kidney and renal pedicle were treated in the same way. After the renal pedicle vessel was blocked for 45 minutes, the color of the kidney turned purple and black, indicating that the clipping was successful. After the vascular clamp was released, the kidney showed mottled changes, indicating that the blood perfusion was restored....

Embodiment 3

[0056] Example 3: Effect of HBSP on renal tubular necrosis caused by renal ischemia-reperfusion injury in rats:

[0057] a. Paraffin-embedded the rat kidney tissue specimens obtained in Experiment 1, made sections, and stained with conventional HE methods.

[0058] b. Observe the pathological changes of kidney tissue in each group under the light microscope, and grade the renal tubular injury pathologically (0=normal kidney; 1=minimal damage, involving 75% of the cortex and outer medulla).

[0059] Such as Figure 6 Shown is the HE staining and damage pathological score (200x) map of kidney tissue section (compared with Vehicle group, *P ), the experimental results showed that the rats in the IR group showed obvious pathological changes in the renal tubules, such as blurred boundaries, swelling, vacuolar degeneration and necrosis, etc., while the pathological changes in the renal tubules of the rats in the HBSP treatment group were significantly alleviated, and the renal tubu...

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Abstract

The invention relates to a short peptide having a kidney protection function as well as a preparation method and application thereof. The short peptide having the kidney protection function is characterized in that the amino acid sequence of the short peptide is QEQLERALNSS. The preparation method is characterized by comprising the following steps: successively coupling Ser, Asn, Leu, Ala, Arg, Glu, Leu, Gln, Glu and Gln on serine which is based on resin as a vector; after cracking, and settling with ice ether so as to obtain coarse peptide; and purifying with a high performance liquid chromatography (HPLC) so as to obtain the product. The invention also discloses application of the short peptide having the kidney protection function in the aspects of preparing formulations for treating renal ischemia-reperfusion injury along with acute tubular necrosis, local renal tissue inflammation and renal tubular epithelium cell apoptosis which are caused by the renal ischemia-reperfusion injury. The invention provides a novel clinic medicament for preventing and treating the renal ischemia-reperfusion injury, thereby bringing good news for patients suffering renal transplant.

Description

technical field [0001] The present invention relates to a short peptide with renal protective effect and its preparation method and application. Its sequence and structure are derived from the surface peptide segment (Helix B surface peptide, HBSP) of erythropoietin (EPO) B helix. Background technique [0002] Renal ischemia and hypoxia lead to insufficient energy metabolism and excessive ATP depletion, and a large number of oxygen free radicals are produced during reperfusion, both of which can lead to apoptosis of renal tubular epithelial cells and severely damage renal function. At the same time, in the process of ischemia, a large number of inflammatory cells are recruited into the renal tubulointerstitium and release various inflammatory mediators, which not only cause direct damage to renal tissue, but also cause damage to renal vascular endothelial cells and enhance immunogenicity, and further lead to immune vascular injury. Clinically, the main causes of renal ische...

Claims

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Application Information

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IPC IPC(8): C07K7/06C07K1/20C07K1/06C07K1/04A61K38/08A61P13/12A61P29/00
Inventor 朱同玉戎瑞明杨橙胡林昆赵天林淼薛寅佳
Owner ZHONGSHAN HOSPITAL FUDAN UNIV
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