Perfusion type bioartificial liver reactor based on double-layered nitrocellulose membrane

Abstract

The invention discloses a perfusion type bioartificial liver reactor based on a double-layered nitrocellulose membrane, which comprises a box body and a cell scaffold arranged in an intracavity of the box body, wherein a liquid inlet, a liquid outlet, an air inlet and an air outlet are arranged on the box body, and the cell scaffold is double-layered nitrocellulose membranes; the two long sides of the double-layered nitrocellulose membranes cling to the inner walls of the front side and the back side of the box body, and the two ends of the double-layered nitrocellulose membranes are fixed onthe inner walls of the left side and the right side of the box body; and the double-layered nitrocellulose membranes divide the intracavity of the box body into upper and lower independent spaces, and a culture medium flows in the upper and lower independent spaces to exchange substances. An interval space between the two layers of the double-layered nitrocellulose membrane is used as a three-dimensional growth space of liver cells, and the cells grow in the interval space, so that substances are fully transmitted and the damage of shearing force to the cells when the liquid flows can be simultaneously avoided, and simultaneously the immune isolation effect can be achieved.

Description

technical field [0001] The invention relates to a bioartificial liver reactor, in particular to a bilayer nitrocellulose membrane perfusion bioartificial liver reactor. Background technique [0002] The bioreactor is the core device of the bioartificial liver system. It is an artificial organ that provides reliable over-supportive therapy for patients with liver failure. It has become an important means for patients with liver failure to wait for liver transplantation or provide a transition through their own liver regeneration. It is an essential supplement for liver transplantation and has broad application prospects in the treatment of liver failure. The artificial liver support system bioreactor must have two basic functions, one is to provide a good growth and metabolic microenvironment for liver cells, and the other is to provide an ideal environment for the interaction between blood or plasma and liver cells and material exchange in patients with liver failure. There...

AI Technical Summary

Problems solved by technology

Among them, the perfusion stent bioreactor has a good hepatocyte culture effect, but lacks immune barrier and uneven perfusion

Hollow fiber bioreactor is the most researched type of reactor, which has strong immune barrier and material transfer function, but the distribution of hepatocytes in this reactor is uneven, easy to deposit at the bottom, and it is difficult to provide three-dimensional hepatocyte culture Microenvironment

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