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Shenshitong quality control method

An inspection method, the technology of kidney stone pass, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of poor effect, weak characteristics, lack of specificity, etc., to prevent counterfeit and shoddy products, rapid qualitative identification, special effect

Active Publication Date: 2012-09-26
GUANGZHOU HANFANG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above-mentioned identification has the disadvantages of weak characteristics and poor effect: quercetin is effective in desmodium, It is present in the storage, and it is lack of specificity to identify it as a reference substance. Wang Buliuxing and The identification effect of Achyranthes bidentata is very poor; the identification repeatability of Achyranthes bidentata is not good, and the separation effect is also poor; the identification methods of Desmodium, Salvia, and Corydalis
At present, there is no method that can accurately determine the content of total flavonoids in Shenshitong preparations

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1 Methodological verification of the testing method of the present invention

[0030] (1) Accuracy test

[0031] Such as figure 2 As shown, when determining the total flavonoid content in Shenshitong extract or Shenshitong preparation, the rutin standard solution and the test solution have a common absorption peak at 273nm wavelength, indicating that this method is suitable for the determination of total flavonoids in Shenshitong The accuracy test shows that the recovery rate of this method can reach 100.1%, and the measurement results are reliable, as shown in Table 1.

[0032] Table 1 Accuracy test results

[0033]

[0034]

[0035] (2) Precision test

[0036] The precision test shows that the method has good precision, as shown in Table 2.

[0037] Table 2 precision test results

[0038]

[0039] (3) Stability test

[0040] The time-coursre test showed that the absorbance value did not change within 180 minutes, as shown in Table 3.

[004...

Embodiment 2

[0048] The inspection method of embodiment 2 Shenshitong extract

[0049] It includes the steps of ingredient identification and determination of total flavonoid content.

[0050] A. Composition identification:

[0051] (1) Take 4g of this product, evaporate to dryness, add 30ml of 65% ethanol, ultrasonically treat for 30 minutes, filter, evaporate the filtrate to dryness, add 10ml of water to dissolve the residue, shake and extract 5 times with ether, 10ml each time, discard the ether solution , then shake and extract with 10ml of ethyl acetate, evaporate the ethyl acetate extract to dryness, add 1ml of 95% ethanol to the residue to dissolve, and use it as the test solution; take 1g of Desmodium as the reference medicinal material, and prepare the reference medicinal material solution in the same way. Take 10 μl of each of the above two solutions, spot them on the same polyamide film, use glacial acetic acid-water (2:1) as developer, develop 15 cm, take it out, dry it in the...

Embodiment 3

[0063] Example 3 The inspection method of Shenshitong Granules (specification: 15 grams per bag, containing 7.65 grams of crude drug) includes the steps of component identification and determination of total flavonoid content.

[0064] A. Composition identification:

[0065] (1) Take 20g of this product, grind it finely, add 50ml of 65% ethanol, ultrasonically treat (power 250W, frequency 33KHz) for 30 minutes, filter, evaporate the filtrate to dryness, add 30ml of water to the residue to dissolve, shake and extract 5 times with ether, 30ml each time, discard the diethyl ether solution, then shake and extract with 30ml ethyl acetate, evaporate the ethyl acetate solution to dryness, add 1ml of 95% ethanol to the residue to dissolve, and use it as the test solution. Take another 1 g of Desmodium as reference medicinal material, and prepare the reference medicinal material solution in the same way. Take 4 μl of each of the above two solutions, spot them on the same polyamide fil...

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Abstract

The invention discloses a Shenshitong quality test method which establishes a thin-layer chromatography (TLC) for identifying desmodium, radix aucklandiae and salvia miltiorrhiza in Shenshitong preparation by taking desmodium medicinal material, radix aucklandiae medicinal material and salvianic acid A sodium as cross reference, and TLC conditions of rhizoma corydalis are improved. A content measuring method for total flavonoids at ultraviolet 273 plus or minus 2 nm by taking rutin as the cross reference is newly established. In the Shenshitong quality control method, on the basis of the original standard, the TLC for identifying the desmodium, the radix aucklandiae, the salvia miltiorrhiza and the rhizoma corydalis is added, so as to accurately and quickly conduct qualitative identification, with strong specificity and good reproducibility; and by adopting the content measuring method for total flavonoids, the quality of the preparation can be comprehensively and accurately reflected; therefore, the Shenshitong quality control method can effectively control the quality of Shenshitong products to prevent fake and inferior products, and also can effectively test the Shenshitong preparation, including intermediate product Shenshitong extract and tablet, capsule, pellet, pill and the like prepared by the formula.

Description

technical field [0001] The present invention relates to a method for testing Chinese patent medicines, in particular to a method for testing Shenshitong, and more particularly to a method for testing Shenshitong extract or Shenshitong preparation. Background technique [0002] Shenshitong Granules are included in the second volume of "Ministry of Health Drug Standards for Traditional Chinese Medicine Prescriptions". The prescription consists of 100g of Desmodium Grass, 100g of Wang Buliuxing (stir-fried), Consists of 60g of storage, 50g of Qumai, 40g of Haijinsha, 40g of Danshen, 40g of Chicken Inner Gold (hot), 30g of Corydalis Corydalis (made with vinegar), 30g of Achyranthes bidentata, and 20g of woody fragrance. The preparation process is as follows: put the above ten flavors, Haijinsha and Wangbuliuxing into a cloth bag, add water to decoct with the other eight flavors such as Desmodium, and decoct twice, the first time is 2.5 hours, the second time is 1.5 hours, the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/90A61K35/37A61K35/57
Inventor 罗爱勤杜松叶健文
Owner GUANGZHOU HANFANG PHARMA
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