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Bone growth factor controlled release type bone repairing material as well as preparation method and applications thereof

A bone growth factor and repair material technology, applied in the field of bone growth factor controlled-release bone repair materials and its preparation, can solve the conditions of poor long-term sustained release of bone growth factor and difficulty in providing synergistic effects of multiple growth factors, etc. To achieve the effect of exerting osteoinductive effect, promoting bone tissue regeneration and functional reconstruction, and meeting the requirements of bone defect repair

Active Publication Date: 2011-08-24
广州暨南大学科技园管理有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still poor long-term slow-release effects of bone growth factors, and bone growth factors can only be released in a single mode, which makes it difficult to provide conditions for the synergistic effect of multiple growth factors required for bone tissue regeneration in physiological environments

Method used

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  • Bone growth factor controlled release type bone repairing material as well as preparation method and applications thereof
  • Bone growth factor controlled release type bone repairing material as well as preparation method and applications thereof
  • Bone growth factor controlled release type bone repairing material as well as preparation method and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 Controlled release BMP-2 / biological bone repair material

[0037] (1) Weigh 1.0 g of chitosan dissolved in 0.5% (v / v) acetic acid solution, prepare a chitosan solution with a concentration of 0.1% by mass and volume, and then stir (rotate at 800r / min), press The mass ratio of bone morphogenetic protein-2 (BMP-2) to chitosan is 0.05:1 and BMP-2 is added and mixed evenly;

[0038] (2) Weigh 1.0g of sodium hyaluronate, dissolve it in deionized water, and prepare a solution with a concentration of 0.1% by weight;

[0039] (3) Weigh 0.5g of the biological porous bone repair material (sample from Guangdong Guanhao Biotechnology Co., Ltd.) and immerse it in the final solution prepared in step (1) under negative pressure (0.01MPa), keep it for 1 hour, and take it out to dry ;

[0040] (4) Immerse the porous bone repair material processed in step (3) into the final solution made in step (2) under negative pressure (0.1MPa), keep it for 1 hour, take it out to dry, and then ste...

Embodiment 2

[0042] Example 2 Controlled-release BMP-2 / bFGF / TCP bone repair material

[0043] (1) Weigh 1.0g of chitosan dissolved in 1.0% (v / v) acetic acid solution, prepare a solution with a concentration of 0.05% by mass and volume, and then press BMP-2 under stirring (speed 3000r / min) Add BMP-2 and bFGF in a ratio of 0.1:0.001:1 with the mass ratio of bFGF to chitosan, and mix well;

[0044] (2) Weigh 1.0 g of carboxymethyl chitosan and dissolve it in deionized water to prepare a solution with a concentration of 0.05% by mass and volume;

[0045] (3) Weigh 0.5g calcium-phosphorus bioceramic porous bone repair material TCP (Wuhan Warwick Biomaterials Engineering Co., Ltd. β-TCP artificial bone, trade name: Biomet artificial bone) immersion step (1) under negative pressure (0.1MPa) ) In the prepared solution, keep for 2 hours, take out and dry;

[0046] (4) Immerse the porous bone repair material processed in step (3) into the solution made in step (2) under negative pressure (0.01MPa), keep fo...

Embodiment 3

[0048] Example 3: Controlled release BMP-2 / bFGF / biological bone repair material

[0049] (1) Weigh 1.0g of chitosan dissolved in 1.0% (v / v) acetic acid solution, prepare a solution with a concentration of 0.05% by mass and volume, and then press BMP-2 under stirring (speed 2000r / min) Add BMP-2 with a mass ratio of 0.1:1 to chitosan and mix well;

[0050] (2) Weigh 1.0g of hyaluronic acid and dissolve it in deionized water to prepare a solution with a concentration of 0.05% by mass and volume. Then, under stirring, add bFGF in a ratio of 0.001:1 by mass of bFGF to hyaluronic acid. ,well mixed;

[0051] (3) Weigh 0.5g of the biological porous bone repair material and immerse it in the solution made in step (1) under negative pressure (0.1MPa), keep it for 1 hour, and take it out to dry;

[0052] (4) Immerse the porous bone repair material processed in step (3) into the solution made in step (2) under negative pressure (0.1MPa), keep it for 1 hour, take it out to dry, and then sterilize...

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Abstract

The invention discloses a bone growth factor controlled release type bone repairing material as well as a preparation method and applications thereof. The bone repairing material provided by the invention is characterized in that the pore surface of a porous bone repairing inorganic material is coated with two layers of membranes from inside to outside, wherein the first-layer membrane is mainly composed of a bone growth factor and chitosan; and the second-layer membrane is formed by mixing a bone growth factor with biodegradability poly anions at a mass ratio of (0-0.001):1. The preparation method provided by the invention comprises the following steps: soaking the porous bone repairing inorganic material into a bone growth factor / chitosan solution, and airing; and soaking in a bone growth factor / biodegradability poly anion aqueous solution, and airing to obtain the bone growth factor controlled release type bone repairing material. The preparation method provided by the invention is simple; and the obtained bone repairing material has good mechanical property and biocompatibility, and ensures that the continuous slow united release or sequential release of various bone growth factors can be realized, and the repairing effect of a bone defect tissue is improved, thereby having a good clinical application prospect in bone repairing.

Description

Technical field [0001] The invention belongs to the technical field of biomedical materials, and particularly relates to a bone growth factor controlled-release bone repair material and a preparation method and application thereof. Background technique [0002] Due to various traumas and diseases, bone injury and bone defects and other hard tissue diseases are frequently-occurring and common diseases worldwide. Bone graft repair is a necessary treatment for providing support, filling the bone cavity, and accelerating bone defect healing. Clinically, it is the most widely used transplant technique after blood transfusion. Currently, the clinical treatment methods used for bone repair in the world include autologous bone transplantation, allogeneic bone transplantation, artificial bone substitutes and so on. Autologous bone transplantation has limitations such as damage to the donor site, insufficient bone graft volume, and inability to prepare special shapes. Allogeneic bone is p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/54A61L27/56A61L27/10A61L27/12A61L27/36
Inventor 汪炬曾戎王丁丁屠美孔杰
Owner 广州暨南大学科技园管理有限公司
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