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Mineralized corn protein fibrous membrane and preparation method thereof

A corn gluten fiber, corn gluten technology, applied in fiber processing, fiber chemical characteristics, single-component protein rayon and other directions, can solve the complex purification process of natural polymer collagen, and the safety and effect of recombinant human collagen are yet to be solved. Inspection and high cost problems, to achieve the effect of wide source of raw materials, good biocompatibility and biodegradability, and low cost

Inactive Publication Date: 2011-08-17
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the purification process of natural macromolecular collagen is complicated and costly, and there are certain safety problems in collagen derived from animals. Need to go through many steps such as desericin, freeze drying, etc.

Method used

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  • Mineralized corn protein fibrous membrane and preparation method thereof
  • Mineralized corn protein fibrous membrane and preparation method thereof
  • Mineralized corn protein fibrous membrane and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Dissolve 3 grams of zein in 10 mL of a mixed solvent of ethanol and water, the volume ratio of ethanol and water is 70:30, and make a 30% (w / v) polymer solution, add it to the storage tank, pass A pump or conduit is connected to the spray head,

[0029] Perform electrospinning. The spray head is connected to a DC high-voltage electric field, the voltage is positive 15 kV, the distance between the spray head and the receiving object is controlled at 10 cm, the solution flow rate is 6 mL / h, and the receiving device is grounded. The zein fibers are randomly stacked to obtain a fibrous film. —4—

[0030] Soak the zein fiber film in 0.01 mol / L acetic acid solution for 30 minutes, 0.5 mol / L calcium chloride solution for 30 minutes, and 0.3 mol / L ammonium dihydrogen phosphate solution for 30 minutes. After the soaking is completed, the fiber membrane is taken out, and after repeated water washing and freeze-drying, the mineralized zein fiber membrane is obtained. ...

Embodiment 2

[0031] Example 2 Dissolve 5 grams of zein in 10 mL of a mixed solvent of methanol and water, the volume ratio of methanol and water is 60:40, make a 50% (w / v) polymer solution, add it to the storage tank, pass A pump or catheter is connected to the spray head for electrospinning. The spray head is connected to a DC high voltage electric field, the voltage is positive 10 kV, the distance between the spray head and the receiving object is controlled at 10 cm, the solution flow rate is 20 mL / h, and the receiving device is grounded. The zein fibers are randomly stacked to obtain a fibrous film. After the zein fiber membrane was crosslinked in 2.5 wt% glutaraldehyde solution for 24 hours, it was soaked in 0.02 mol / L acetic acid solution for 30 minutes. The cross-linked zein fiber membrane was immersed in 0.5 mol / L calcium chloride solution for 30 minutes, followed by 0.3 mol / L sodium dihydrogen phosphate solution for 30 minutes, and this was regarded as a cycle, soaking for 3 cycl...

Embodiment 3

[0032] Example 3 Dissolve 4 grams of zein in 10 mL of a mixed solvent of ethanol and water. The volume ratio of ethanol and water is 70:30 to form a 40% (w / v) polymer solution. Add 1.5 mL of 0.05 mg / mL bone morphogenetic protein (BMP-2) in phosphate buffered saline (PBS), stir well. Add the mixed solution into the storage tank, connect it to the spray head through a pump or catheter, and perform electrospinning. The spray head was connected to a DC high-voltage electric field with a voltage of minus 12 kV, the distance between the spray head and the receiver was controlled at 10 cm, the solution flow rate was 6 mL / h, and the receiving device was grounded. After immersing zein fiber in 0.01 mol / L acetic acid solution for 20 minutes, soak in 0.15 mol / L calcium chloride solution for 15 minutes, then in 0.09 mol / L ammonium dihydrogen phosphate solution for 15 minutes, and use this as a cycle , Soak for 3 cycles. After the soaking is completed, the fiber membrane is taken out, a...

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Abstract

The invention discloses mineralized corn protein fibrous membrane which is formed by the irregular accumulation of nano-fiber containing an organic component and mineral, wherein the organic component is corn protein or corn protein containing additive, and the mineral is calcium phosphate crystal. The diameter of the corn protein fiber or corn protein fiber containing additive is 20 nanometers to 10 microns, and the pore size of the fibrous membrane is 100 nanometers to 100 microns. A preparation method of the mineralized corn protein fibrous membrane comprises the following steps: dissolving the corn protein in a solvent; and performing electric spinning to prepare the fibrous membrane of irregular accumulation; sequentially soaking the prepared fibrous membrane in acetic acid solution, calcium ion-containing solution and phosphate radical-containing solution for certain time, wherein the molar ratio of the calcium ions to the phosphate radical ions is (1:2)-(3:1); and repeatedly soaking, washing and freeze-drying the fibrous membrane to obtain the mineralized corn protein fibrous membrane.

Description

technical field [0001] The invention relates to a mineralized zein fiber membrane and a preparation method thereof, relates to a mineralized zein fiber membrane for bone tissue repair and regeneration and a preparation method thereof, and belongs to the field of biomedical materials. Background technique [0002] Bone is a highly mineralized tissue in the human body with hydroxyapatite crystals as the main inorganic component. Mineralization of bone begins with the formation of a collagen network, which self-assembles into a three-dimensional framework in which tiny hydroxyapatite crystals nucleate in regularly spaced grooves on the collagen fibrils. In bone tissue, due to the interaction between hydroxyapatite and protein, a special advanced self-assembly structure is formed, which shows excellent mechanical properties and biological functions such as compressive or tensile resistance. Biomimetic mineralization in terms of composition, structure and function has always bee...

Claims

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Application Information

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IPC IPC(8): D04H1/72D01D5/00D01F4/00D01F1/10D01F11/02
Inventor 姚琛李新松吴菲菲李月玲
Owner SOUTHEAST UNIV
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