Device for automatically regulating and controlling oxidation-reduction potential and application thereof
A potentiometric and automatic technology, applied in biochemical cleaning devices, enzymology/microbiology devices, specific-purpose bioreactors/fermenters, etc., can solve problems such as high knowledge and experience requirements, large fluctuations, and high operating intensity , to optimize the production performance of the strain, increase the fermentation output, and improve the synergistic effect
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Embodiment 1
[0050] Embodiment 1. A device for automatically regulating oxidation-reduction potential and its application method
[0051] 1. A device that automatically regulates the redox potential
[0052] Such as figure 1 As shown, a device for automatically regulating the oxidation-reduction potential includes a fermentation tank 8, an ORP electrode 9 arranged in a fermentation tank 8, a signal normalization converter 10, an I / O module 11, a central processing unit 1, and an I / O module 3. Electric signal control pulse valve 5, peristaltic pump 4.
[0053] Fermentation tank 8 is a place for fermentation. The ORP electrode 9 is used to measure the oxidation-reduction potential in the fermenter 8 and can generate an ORP electrode signal. Signal standardization converter 10 and I / O module 11 are arranged between ORP electrode 9 and central processing unit 1, wherein, signal standardization converter 10 can convert the ORP electrode signal of ORP electrode 9 to the standard signal of 4-2...
Embodiment 2
[0076] Embodiment 2, using the device online monitoring of the automatic regulation of redox potential of embodiment 1
[0077] As described in 1 of step 2 of Example 1, the device for automatically regulating the oxidation-reduction potential of the present invention can monitor and automatically record the ORP change of the measured system on-line, collect data, and the data interval can be set arbitrarily by the user. The time interval is It can be greater than 1 second. The collected data is recorded by the system to generate an automatic report, which can be opened in excel format for users to call.
[0078] This example takes the fermentation of Klebsiella (Klebsiella) as an example. This system is used to detect and record two different fermentation conditions of Klebsiella oxytoca (Klebsiella oxytoca): Klebsiella oxytoca ATCC 43165 (preserved by the American ATCC strain bank, preserved No. 43165; China Industrial Microorganism Culture Collection Management Center CICC...
Embodiment 3
[0085] Embodiment 3: Utilize the device for automatically regulating the redox potential of embodiment 1 to control the flow rate of air, oxygen and / or nitrogen to automatically regulate ORP
[0086] As described in 2 of step 2 of Example 1, the device for automatically regulating the redox potential of the present invention can adjust the air / nitrogen flow into the system, and use glycerol as a substrate for Klebsiella oxytoca ATCC 43165 The fermentation process that produces 1,3-propanediol is regulated.
[0087] Seed medium ( / L): Dipotassium hydrogen phosphate (K 2 HPO 4 ·3H 2 O) 4.45g, potassium dihydrogen phosphate (KH 2 PO 4 ) 1.3g, ammonium sulfate ((NH4) 2 SO 4 ) 3.0g, magnesium sulfate (MgSO 4 ·7H 2 O) 0.2g, calcium carbonate (CaCO 3 ) 2.0g, yeast extract (Yeast extract) 1.0g, glucose 20g, trace element solution (Trace Elemental Solution, hereinafter referred to as TES) 2.0ml, iron solution (Fe 2+ solution) 2.0ml, pH 7.0.
[0088] Fermentation medium ( / L): ...
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