Degradable polyamino acid derivative as well as preparation method and application thereof

Abstract

The invention provides a degradable polyamino acid derivative which has a molecular weight of 30,000-40,000Da. A series of primary, secondary and tertiary amino groups in a certain proportion are introduced to a side chain so that the degradable polyamino acid derivative has very strong pH buffering capability and higher capability of transferring and expressing a plasma DNA; and the DNA binding capability, the transfection efficiency and the cytotoxicity can be adjusted and optimized through adjusting the proportion of the primary, secondary and tertiary amino groups. The synthesizing steps are simple and easy to operate, and the safety is high; the structural frame of the degradable polyamino acid derivative is polyamino acid which has the advantages of degradability, low toxicity and the like, can be applied to preparation of a degradable polycation nano gene carrier material and has a structural formula as the specification.

Description

technical field The invention belongs to the field of biological materials, and in particular relates to a series of derivatives of biodegradable polyamino acid materials that efficiently transfer and express genes, and their application in the preparation of degradable polycation nanometer gene carrier materials. technical background Gene therapy achieves the purpose of treatment by introducing normal genes into target cells to treat congenital or acquired genetic defects. In gene therapy, if naked DNA is directly injected into target tissues or cells, the transfection efficiency is often very low. This is because the uptake rate of exogenous DNA by cells is very low, and exogenous DNA is easily cleared by organisms or even degraded by itself. Therefore, a safe and efficient gene carrier has become an essential part of gene therapy. Although the virus vector has high efficiency, it has great security risks. Non-viral gene vectors have many advantages, such as low immunog...

AI Technical Summary

Problems solved by technology

However, its non-degradability and high toxicity inhibit its application in vivo.