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Method for preparing swine blood active peptide by microwave-accelerated enzymolysis

A microwave-assisted and active peptide technology, which is applied in the field of rapid preparation of pig blood active peptides, can solve the problem of reduced nutritional value of amino acids

Inactive Publication Date: 2011-08-10
JILIN UNIV
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  • Summary
  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

At present, protein powder in industrial production mainly adopts acid-base method or enzymatic hydrolysis method, but the use of acid-base method will reduce the nutritional value of amino acids, while using enzymatic hydrolysis method, the enzymatic hydrolysis process takes more than ten hours or even longer

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0013] 1 Separation of blood cells

[0014] Add 0.33% trisodium citrate to anticoagulant fresh pig blood qualified for quarantine, and stir slowly. Use a high-speed refrigerated centrifuge to separate blood, centrifuge at 3000rpm for 15min at 4°C, pour off the supernatant, add an equal volume of 0.9% saline and centrifuge again, repeat the above cleaning steps twice to obtain purer blood cells.

[0015] 2 Ultrasonic wall breaking

[0016] The cell wall of the blood cells was broken by ultrasonic waves, the power was adjusted to 400w, the intermittent ratio was 2:1, twice the volume of deionized water was added, and the ultrasonic treatment was performed for 20 minutes. The wall-breaking operation was carried out at room temperature. Measure above-mentioned solution with ultraviolet-visible spectrophotometer, absorbance rises from 0.102 to 0.408, and breaking effect is remarkable.

[0017] 3 Microwave-assisted enzymatic hydrolysis

[0018] Microwave-assisted enzymatic hydro...

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Abstract

The invention discloses a method for preparing swine blood active peptide by microwave-accelerated enzymolysis, belonging to the field of finish and deep processing and comprehensive utilization of animal byproducts. The method comprises the steps of anticoagulation of swine blood, centrifugal separation, ultrasonic wall breaking, microwave-accelerated enzymolysis, secondary enzymolysis, inactivation, membrane filtration and spray drying. The product can be used as a raw material or additive for various foods. Compared with the traditional methods, the protein molecules move violently due to microwave-accelerated enzymolysis, the enzymolysis velocity can be obviously improved and the enzymolysis time is shortened. The method has simple process, low cost and environment friendliness and is suitable for industrial production of deep processing of swine blood.

Description

technical field [0001] The invention belongs to a method for rapidly preparing porcine blood active peptide, and belongs to the technical field of deep processing and comprehensive utilization of livestock and poultry by-products. Background technique [0002] my country is rich in pig blood resources, but the waste of pig blood is serious in my country. At present, it is roughly processed into blood powder or blood tofu, and the intensive processing is mainly focused on the production of heme, SOD and iron supplement products. Pig blood has high protein content and can be used to produce and process active peptide products. Active peptides not only have nutritional function and biological activity, but also have the characteristics of being quickly absorbed by the intestinal tract. At present, the protein powder produced in industry mainly adopts the acid-base method or enzymatic hydrolysis method, but the use of acid-base method will reduce the nutritional value of amino...

Claims

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Application Information

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IPC IPC(8): C12P21/06
Inventor 潘风光李洪山刘静波赵娅娅吴涵秦亚楠方珍
Owner JILIN UNIV
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