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Preparation method for tissue engineering scaffold material

A technology of tissue engineering scaffold and sucrose, which is applied in medical science, prosthesis, etc., can solve the problems of poor connectivity between pores and high obturator rate, and achieve the effects of low obturator rate, increased porosity, and wide connection

Inactive Publication Date: 2011-07-06
CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the particle porogenation method reported above, since the particles exist independently, the connectivity between the formed pores is poor, and the closed pore rate is high.

Method used

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  • Preparation method for tissue engineering scaffold material
  • Preparation method for tissue engineering scaffold material
  • Preparation method for tissue engineering scaffold material

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preparation example Construction

[0030] The embodiment of the present invention discloses a preparation method of a tissue engineering scaffold material, which is characterized in that it comprises:

[0031] Step a) mixing sucrose fibers with particles to obtain a mixture, said particles being sucrose particles and / or salt particles;

[0032] Step b) mixing the mixture obtained in step a) with a degradable polymer and a solvent, and then freezing to obtain a compound, and removing the compound and solvent obtained in step a) from the compound to obtain a tissue engineering scaffold material.

[0033] In step a) of the present invention, the sucrose fiber is preferably prepared according to the following method:

[0034] Spinning sucrose to obtain sucrose fibers;

[0035] The sucrose fibers are sheared.

[0036] The above-mentioned spinning method is preferably a melt spinning method, a solution spinning method, a melt spinning method, an electrospinning method, a disk spinning method or a screw extrusion me...

Embodiment 1

[0045] Making sucrose into sucrose fibers with a diameter of 50 μm by spinning disk spinning method to provide sucrose particles with a particle size of 300 μm;

[0046] Dissolve polylactide-glycolide (PLGA) in acetone at a mass volume ratio of 5% g / ml, and stir magnetically to obtain a polylactide-glycolide solution;

[0047]The sucrose fiber is shredded, mixed with the sucrose particles at a weight ratio of 1:1, and then mixed with the polylactide-glycolide solution, and the weight ratio of the PLGA to the sucrose fiber and particles is 1:2:2, stir evenly, freeze at -20°C, then use a vacuum pump to sublimate acetone in a 4°C freezer to obtain a complex, put the obtained complex into distilled water, and constantly replace the distilled water to make sucrose fiber and sucrose The particles are dissolved and dried to obtain tissue engineering scaffold materials. The scanning electron microscope picture of the tissue engineering scaffold material prepared in this embodiment is...

Embodiment 2

[0049] Making sucrose into sucrose fibers with a diameter of 50 μm by spinning disk spinning method to provide sucrose particles with a particle size of 250 μm;

[0050] Dissolve polylactide-glycolide (PLGA) in acetone at a mass volume ratio of 5% g / ml, and stir magnetically to obtain a polylactide-glycolide solution;

[0051] The sucrose fiber is shredded, mixed with the sucrose particles at a weight ratio of 1:1, and then mixed with the polylactide-glycolide solution, and the weight ratio of the PLGA to the sucrose fiber and particles is 1:4:4, stir evenly, freeze at -20°C, then use a vacuum pump to sublimate acetone in a 4°C freezer to obtain a complex, put the obtained complex into distilled water, and constantly replace the distilled water to make sucrose fiber and sucrose The particles are dissolved and dried to obtain tissue engineering scaffold materials. The scanning electron microscope picture of the tissue engineering scaffold material prepared in this embodiment i...

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Abstract

The embodiment of the invention discloses a preparation method for a tissue engineering scaffold material. The method comprises the following steps of: mixing sucrose fibers with particles to obtain a mixture; mixing the mixture with degradable polymer and a solvent; freezing to obtain a complex; and removing the solvent and the mixture from the complex to obtain the tissue engineering scaffold material. A fiber and particle joint pore forming method is introduced, and the sucrose fibers and the particles are used as a pore forming agent. Compared with a particle pore forming method in the prior art, the pore forming method has the advantages that: the connectivity among formed pores because of wider connection among the fibers is greatly improved, the porosity is improved, and the pore closing rate is lower. Experimental results show that the compression strength of the tissue engineering scaffold material prepared by the fiber and particle joint pore forming method is between 0.3 and 0.32MPa, and the strength meets the requirements on the tissue engineering scaffold material.

Description

technical field [0001] The invention relates to the technical field of tissue engineering, in particular to a method for preparing a tissue engineering scaffold material. Background technique [0002] Tissue engineering is a science that applies the principles of cell biology, biomaterials and engineering to study bioactive substitutes for repairing or improving the structure of human diseased tissues or organs, with the purpose of repairing and regenerating damaged tissues or organs , to help patients restore the function of damaged tissues. [0003] The basic principle and method of tissue engineering is to plant osteoblasts obtained from the soft and hard tissues of patients in culture medium, and to amplify the high-concentration tissue cells cultured in vitro and adsorb them on a biocompatible tissue. And can be gradually degraded and absorbed by the human body on the extracellular matrix material. The matrix material is formed into a prefabricated tissue engineering ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/18
Inventor 章培标王宗良陈学思崔立国王宇
Owner CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
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