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Preparation method and application of extracts from natural plants

A technology for natural plants and extracts, applied in the field of preparation of natural plant extracts, can solve the problems of low extraction rate and transfer rate of total flavonoids, excessive heavy metal content in the extract, many operation steps and elution steps, etc. The effect of short time, low production cost and small amount of solvent

Active Publication Date: 2011-06-29
NANNING GODSEND BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many operating steps and elution steps in the above operation, large amount of organic solvent, low yield, and high production cost. In particular, there are many heating and concentration links in the extraction and purification process, which easily cause damage or loss of active ingredients. Total flavonoids The extraction rate and transfer rate are not high, and toxic and harmful solvents such as ethyl acetate, n-butanol, chloroform, and methanol are often used, causing pollution to the environment, and the heavy metal content in the extract often exceeds the standard

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1) Raw material processing: crush the dried epiphytic mesinica into coarse powder, and pass through a 20-mesh sieve;

[0028] 2) Extraction: put the medicinal material into the percolation cylinder, soak in solvent for 5 hours, then use 60% aqueous ethanol as solvent for percolation extraction. If the weight of the raw material is 1kg, the amount of aqueous ethanol is 10 liters, and the flow rate is 12 mL min -1 The speed of collecting percolate;

[0029] 3) Concentration under reduced pressure: put the percolation solution in a water bath at a temperature of 60°C and a vacuum of 0.095 Mpa, and wait until the flow rate of the concentrated solution is 220 mL·min -1 Carry out vacuum thin-film concentration under the condition of condition, recover ethanol, and every milliliter of concentrated solution contains 0.5 gram of original medicinal material;

[0030] 4) Purification with macroporous resin: The obtained concentrate is separated and enriched by Diaion HP-20 macr...

Embodiment 2

[0033]Step 1) Pulverize Epiphytic mesinica into coarse powder, and pass through a 40-mesh sieve; Step 2) Soak in the middle for 6 hours, use 50% aqueous ethanol as solvent for percolation extraction, if the weight of the raw material is 1kg, then the aqueous ethanol The volume is 12 liters, the flow rate is 15 mL min -1 ; In step 3), the temperature of the water bath is 70°C, the degree of vacuum is 0.090Mpa, the flow rate of the liquid feed is 200 mL·min-1, and each milliliter of the concentrated solution contains 0.6 grams of the original medicinal material; in step 4), the diameter-to-height ratio of the chromatographic column is 1 : 16, use H first 2 O elution, followed by gradient elution with 10% aqueous ethanol, 25% aqueous ethanol, 45% aqueous ethanol, 55% aqueous ethanol, 75% aqueous ethanol, and finally eluted with 70% acetone, combining 25% aqueous ethanol, 45% aqueous ethanol and 55% aqueous ethanol eluent from each part; step 5) in vacuum concentration temperatur...

Embodiment 3

[0035] Step 1) Pulverize Epiphytic mesinica into coarse powder, pass through a 40-mesh sieve; Step 2) Soak in the middle for 7 hours, use 70% aqueous ethanol as solvent for percolation extraction, if the weight of the raw material is 1kg, then the aqueous ethanol The volume is 11 liters, the flow rate is 18 mL min -1 ; In step 3), the temperature of the water bath is 80°C, the vacuum degree is 0.092Mpa, the flow rate of the liquid feed is 250 mL·min-1, and each ml of the concentrated solution contains 0.8 grams of the original medicinal material; the ratio of diameter to height of the chromatographic column in step 4) is 1:17 , first use H 2 O elution, followed by gradient elution with 10% aqueous ethanol, 30% aqueous ethanol, 50% aqueous ethanol, 60% aqueous ethanol, and 80% aqueous ethanol, and finally eluted with 70% acetone, combining 30% aqueous ethanol, 50% aqueous ethanol and 60% aqueous ethanol eluent from each part; step 5) medium vacuum concentration temperature 70°...

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Abstract

A preparation method and application of extracts from natural plants belong to the technical field of phytoextraction. The preparation method comprises the following steps that medinilla arboricola raw material is crashed and screened, and then is extracted through percolation, concentrated through a vacuum film and absorbed and enriched through macroporous resin, so that medinilla arboricola extracts are obtained, and the medinilla arboricola extracts comprise the following components in percentage by weight: 11.5 to 18.6 total flavone and 21.3 to 32.6 total phenolic acid, and has strong bacteriostasis and antioxidation and also has the auxiliary functions of reducing blood pressure and blood sugar, and can serve as food preservatives, food antioxidants and food additives. The extracts have simple preparation process, convenience in operation, low equipment cost, small solvent dosage, no environmental pollution, low production cost, and high yield and functional composition content.

Description

technical field [0001] The invention belongs to the technical field of plant extraction, and specifically relates to a preparation method and application of a natural plant extract. Background technique [0002] Flavonoids are a class of important biologically active substances that widely exist in the plant kingdom. Modern medical research shows that flavonoids have anti-oxidation, anti-aging, free radical scavenging, anti-virus, anti-tumor, antibacterial, and cardiovascular and cerebrovascular protection. , immune regulation and other aspects, is the current research hotspot of natural active ingredients, has a very good development and application value. [0003] Plant polyphenolic compounds are a class of secondary metabolites that widely exist in natural plants, fruits and vegetables, etc., and are a class of natural strong reducing substances, which have stronger antioxidant and free radical scavenging effects than commonly used antioxidants. Plant phenolic acids also...

Claims

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Application Information

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IPC IPC(8): A23L3/3472A23L1/29A61K36/185A61P3/10A61P9/12A23L33/00
Inventor 袁珂吴叶平刘剑波刘华亮
Owner NANNING GODSEND BIOTECH
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