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Application of zedoary cyclic diolefine in preparation of medicine for resisting neoplasm metastasis

A kind of anti-tumor metastasizing technology of zedoary cyclodiene, applied in anti-tumor drugs, drug combinations, pharmaceutical formulas, etc., to achieve the effect of inhibiting tumor metastasis

Inactive Publication Date: 2011-06-22
HANGZHOU MINSHENG PHARM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Yu Xiao et al. reported that zedoary cyclodiene can induce human liver cancer HepG2 cells to arrest at G 2 / M phase, and undergoes apoptosis through the MAPK signaling pathway and the mitochondrial-caspase pathway (Cancer Biology & Therapy 6:7, 1044-1050; 2007); in addition, it has also been reported that fenugreek cyclodiene induces human leukemia HL-60 Apoptosis is accomplished by activating the TNFR1 signaling pathway (Cancer Letters 271 158-166; 2008); so far there is no report on the anti-tumor metastasis of pomzocyclodiene

Method used

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  • Application of zedoary cyclic diolefine in preparation of medicine for resisting neoplasm metastasis
  • Application of zedoary cyclic diolefine in preparation of medicine for resisting neoplasm metastasis

Examples

Experimental program
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preparation example 1

[0013] Preparation example 1: The preparation of zedoary cyclodiene

[0014] Take 1kg of dried rhizomes of Edoctorale Rhizome, and distill them through a steam distillation device to obtain a total of 385g of oil-water mixture, extract with diethyl ether and petroleum ether, separate the oil layer, discard the water layer, and concentrate to obtain 46.5g of zedoary volatile oil. Separation, eluted with petroleum ether, collected in 100ml per bottle, combined 26-42 components, and removed the solvent to obtain 15.6g of yellow crystals, separated on a silica gel column, eluted with petroleum ether, 30ml per bottle, combined 16-28 components , Remove solvent, obtain white crystal 10.7g, HPLC analyzes the purity of this sample and reaches 99.7%, high-resolution mass spectrometry positive ion detection shows the quasi-molecular ion peak [M+H] of sample + It is 217.1583, and its molecular weight is deduced to be 216.1505. The detected elements match, and its elemental composition is...

preparation example 2

[0015] The preparation of preparation example 2 Peng curcuma cyclodiene soft capsule

[0016] 2.4g turmeric cyclodiene

[0017] 0.24mg soybean lecithin

[0018] 0.24mg methylparaben

[0019] Add soybean oil for injection to dissolve the above components and set the volume to 24ml

[0020] Preparation: Take 1000ml of soybean oil for injection, dry heat sterilization at 150°C for 1 hour, let it cool to 25°C, take out part of the oil and add the above-mentioned main ingredients and excipients, stir to dissolve completely, and finally set the volume to 24ml, dry it with No. 6 vertical melting funnel filter, potting in soft capsules, sterilized by ultraviolet light for 60 minutes, making soft capsules containing 200mg of zedoary cyclodiene in each.

experiment example 1

[0021] Experimental Example 1 Inhibition of Curcuma Cyclodiene to Invasion of Tumor Cells in Vitro

[0022] Experimental materials: Bel-7402 liver cancer cell line, BGC-823 gastric cancer cell line, A549 lung cancer cell line, 5637 bladder cancer cell line, MCF-7 breast cancer cell line, Caco-2 colon cancer cell line, OS-RC-2 kidney cell line Cancer cell line, Eca-109 esophageal cancer cell line, GBC-SD gallbladder cancer cell line, HO8910 ovarian cancer cell line, PANC-1 pancreatic cancer cell line, Hela cervical cancer cell line, SW579 thyroid cancer cell line, PC-3 prostate cancer cell line Cancer cell lines, KB oral cancer cell lines: provided by the Shanghai Cell Bank of the Chinese Academy of Sciences, routinely cultured and passaged by the clinical pharmacology laboratory of our company; Cell Invasion Assay KIT (ECM550), Chemicon Company; DMEM, RPMI-1640 medium, Gbico Company; fetal bovine Serum (FBS), Hangzhou Sijiqing Bioengineering Material Company; DMSO, Sigma Compa...

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Abstract

The invention belongs to the field of pharmacy, and provides application of zedoary cyclic diolefine in preparation of a medicine for resisting neoplasm metastasis, and in particular relates to application in the preparation of medicines for resisting neoplasm metastasis, such as liver cancer, gastric cancer, carcinoma of urinary bladder, breast cancer, colon cancer, kidney cancer, esophagus cancer, gallbladder cancer, ovarian cancer, pancreatic cancer, carcinoma of uterine cervix, thyroid cancer, prostatic cancer, oral cancer, lung cancer and the like. The zedoary cyclic diolefine plays a role in resisting the neoplasm metastasis through inhibiting the expression of proteins related to neoplasm cell metastasis.

Description

technical field [0001] The invention relates to the application of zedoary cyclodiene in the preparation of drugs for anti-tumor metastasis. Background technique [0002] Invasion and metastasis are the essential characteristics of malignant tumors. Metastasis allows the spread of local lesions into systemic multifocal disease. When the primary tumor is clinically diagnosed, about 50% of the patients have metastasized to distant sites. The existing methods of surgery, radiotherapy and chemotherapy often fail in the treatment of metastatic cancer, resulting in the deterioration of the patient's condition or death. Invasion and metastasis are the crux of the fatality of malignant tumors. Prevention and treatment of tumor invasion and metastasis is one of the important ways to reduce the mortality rate of tumors. [0003] Tumor invasion refers to the fact that malignant tumor cells detach from the primary site, break through the barrier formed by basement membrane and extrace...

Claims

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Application Information

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IPC IPC(8): A61K31/343A61P35/04
Inventor 孟昭珂郭殿武杨岚冯飞玉黄滨南吴耀东
Owner HANGZHOU MINSHENG PHARM CO LTD
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