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Production method of Bacillus subtilis antimicrobial lipopeptide and application of Bacillus subtilis antimicrobial lipopeptide in piglet feeds

The technology of an antimicrobial lipopeptide and a production method, which is applied in the field of animal husbandry, can solve the problems of low production of food safety antimicrobial peptides, and achieve the effects of improving feed utilization rate and immune function, improving feed utilization rate, improving production and regulating immunity.

Active Publication Date: 2011-06-15
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the problem of food safety caused by the use of antibiotics in feed and the problem of low production of antimicrobial peptides from animal sources

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Using a 5000 L fermenter, the optimal fermentation process conditions after 500 L amplification were used for Bacillus subtilis ( Bacillus subtilis ) fmbJ (preservation number: CGMCC No.0943, see patent publication number: CN1477189) for fermentation production. The optimal fermentation process parameters are: temperature 28-35 ℃, stirring speed 180 r / min, ventilation volume 80 m 3 / h, tank pressure 0.10~0.12Mpa, pH7.0, soybean oil 0.3%.

[0016] Bacillus subtilis ( Bacillus subtilis ) strain fmbJ, seed medium expansion culture, seed medium: tryptone 10.0 g, yeast extract 5.0 g, NaCl 5.0 g, distilled water 1000 ml, pH7.2; volume ratio 2~5% inoculation, 28~35℃, Submerged fermentation in 5000 L fermenter for 30-48 h, liquid fermentation medium: glucose 20.0 g / L, tryptone 5.0 g / L, sodium glutamate 2-5%, MgSO 4 0.5 g / L, KCl 0.5 g / L, KH 2 PO 4 1 g / L, FeSO 4 0.15 mg / L, MnSO 4 5.0 mg / L, CuSO 4 0.16 mg / L, pH7.0; fermentation process parameters: temperature 28-35°...

Embodiment 2

[0019] Using a 5000L fermenter, the optimal fermentation process conditions after 1000 L amplification Bacillus subtilis fmbJ for fermentation production. The optimal fermentation process parameters are: temperature 32°C, stirring speed 180 r / min, ventilation volume 80 m 3 / h, tank pressure 0.10~0.12Mpa, pH7.0, soybean oil 0.3%.

[0020] The industrial centrifuge used is a disc centrifuge with a rotating speed of 6000 r / min to remove bacteria and then dry it by spray drying. The process is as follows: temperature 210°C, fan speed 70 rpm, excipient: maltodextrin: porous starch=6~9:1, at this time, the recovery rate of antimicrobial peptide is 54%, and the antibacterial potency is 20000~ 70000 IU / g dry powder.

Embodiment 3

[0022] A total of 360 healthy long × large binary hybrid piglets weaned at the age of 28 days were selected and randomly divided into 4 treatment groups according to the principle of half male and half female and different sources. Each treatment set up 6 replicates, each replicated 15 piglets, and the weight of piglets in each replicate Similar (P>0.05). Corn-soybean meal was selected as the basic diet, and the nutritional requirements were referred to NRC (1998) "Nutritional Requirements of Pigs", the apparent digestible amino acid pattern in the ileum, diet composition and nutritional components. The treatment groups were the blank control group (group C) fed with basal rations (the basal rations did not contain antibiotics and antimicrobial lipopeptides), and the antibiotic control group (AC) added 5 mg / kg of flavomycin to the basal rations , Test? Add 2000 IU / kg of Bacillus subtilis fmbJ antimicrobial lipopeptide to the basal diet, the pre-test period is 3 days, and the t...

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Abstract

The invention relates to a production method of Bacillus subtilis antimicrobial lipopeptide and application of the Bacillus subtilis antimicrobial lipopeptide in piglet feeds, belonging to the technical fields of feed additives and biology. The method comprises the following steps: carrying out amplification culture on Bacillus subtilis fmbJ in a seed culture medium, inoculating 2-5% of Bacillus subtilis, carrying out submerged fermentation in a 5000L fermentation tank at 28-35 DEG C for 30-48 hours, extracting, and carrying out spray drying to obtain the antimicrobial lipopeptide product. When 4000IU of antimicrobial lipopeptide is added to every 1kg of basic ration for piglets, the antimicrobial lipopeptide increases the utilization ratio of the feed, and has favorable effects on promoting the growth and development of piglets, preventing piglets from diarrhoea and enhancing the immunity of piglets. The Bacillus subtilis strain provided by the invention is a safe microbe, and the antimicrobial product has high safety. In addition, compared with flavomycin used in the existing piglet feeds, the Bacillus subtilis antimicrobial lipopeptide has a better effect, and has important meanings for substituting feed antibiotic additives and ensuring food safety.

Description

technical field [0001] The invention relates to a production method of Bacillus subtilis antibacterial lipopeptide and its application in piglet feed, and belongs to the technical field of animal husbandry and the field of biotechnology in the agricultural field. It involves the use of antimicrobial peptides to replace the addition of antibiotics in feed, on the premise of preventing diarrhea in piglets, reducing the residue of antibiotics in piglets and ensuring food safety. Background technique [0002] The concept of feed safety, namely food safety, has become a consensus in the world. Food and feed belong to the same scope of management in the United States and apply to the same law; in order to ensure food safety, the Danish government has formulated regulations prohibiting the use of antibiotics in feed production; the European Union has banned the use of antibiotics as feed accelerators in animal feeding. With the improvement of people's living standards, the demand ...

Claims

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Application Information

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IPC IPC(8): C12P21/02A23K1/16A23K10/12A23K50/30A23K50/60
Inventor 陆兆新王恬吕凤霞别小妹都海明赵海珍
Owner NANJING AGRICULTURAL UNIVERSITY
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