Method for fixing polypeptide aptamers on surface of cardiovascular implanting device

A technology of polypeptide aptamer and implantation device, which is applied in the field of surface modification of biological decontamination materials, can solve the problems of high late thrombosis risk, incomplete surface endothelialization, etc., and achieve the effect of excellent anticoagulant function.

Inactive Publication Date: 2014-04-09
CO WITH LTD LIABILITY OF MEDICAL SCI +1
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although drug-eluting stents can reduce the incidence of restenosis, they have a relatively high risk of late thrombosis due to incomplete endothelialization of their surface

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for fixing polypeptide aptamers on surface of cardiovascular implanting device
  • Method for fixing polypeptide aptamers on surface of cardiovascular implanting device

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A. Activation treatment

[0027] The sample was placed in 83% phosphoric acid solution, activated in an oven at 120°C for 0.5h, ultrasonically cleaned in deionized water, and dried. Then immerse the sample in 8.3% phosphoric acid solution for 10 minutes, keep it warm at 120°C for 12 hours, ultrasonically clean it with deionized water for 3 times, each time for 10 minutes, and dry it;

[0028] B. Silanization treatment:

[0029] The implanted device after the activation treatment in step A was placed in a 5% APTES / anhydrous tetrahydrofuran solution (V / V) and refluxed for 5 hours. The sample was taken out and continued to reflux in anhydrous tetrahydrofuran for 5 hours. Ultrasonic cleaning, vacuum drying;

[0030] C. Immobilized biotin

[0031] Add 0.1 mg of biotin to 20 mL of 0.001 mol / L 2-morpholineethanesulfonic acid (MES) aqueous solution, and add 1×10 -5 mol N-hydroxysuccinimide (NHS), 1×10 -5 mol 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide (EDC) to activate t...

Embodiment 3

[0062] A. Activation treatment

[0063] Soak the cardiovascular implant device in a 4mol / L NaOH solution at 90°C for 24h, take it out, place it in deionized water at 120°C for 0.5h, and dry it;

[0064] B. Silanization treatment:

[0065] The implanted device after the activation treatment in step A was placed in a 5% APTES / anhydrous tetrahydrofuran solution (V / V) and refluxed for 5 hours. The sample was taken out and continued to reflux in anhydrous tetrahydrofuran for 5 hours. Ultrasonic cleaning, vacuum drying;

[0066] C. Immobilized biotin

[0067] Add 0.1 mg of biotin to 20 mL of 0.001 mol / L 2-morpholineethanesulfonic acid (MES) aqueous solution, and add 1×10 -5 mol N-hydroxysuccinimide (NHS), 1×10 -5 mol 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide (EDC) to activate the carboxyl group on biotin. The silanized sample was immersed in the above solution, reacted at room temperature for 4 hours, ultrasonically cleaned in deionized water, and dried in vacuum.

[0068]...

Embodiment 4

[0075] A. Activation treatment

[0076] Soak the cardiovascular implant device in a 4mol / L NaOH solution at 90°C for 24h, take it out, place it in deionized water at 60°C for 24h, and dry it;

[0077] B. Silanization treatment:

[0078] The implanted device after the activation treatment in step A was placed in a 5% APTES / anhydrous tetrahydrofuran solution (V / V) and refluxed for 5 hours. The sample was taken out and continued to reflux in anhydrous tetrahydrofuran for 5 hours. Ultrasonic cleaning, vacuum drying;

[0079] C. Immobilized biotin

[0080] Add 0.1 mg of biotin to 20 mL of 0.001 mol / L 2-morpholineethanesulfonic acid (MES) aqueous solution, and add 1×10 -5 mol N-hydroxysuccinimide (NHS), 1×10 -5 mol 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide (EDC) to activate the carboxyl group on biotin. The silanized sample was immersed in the above solution, reacted at room temperature for 4 hours, ultrasonically cleaned in deionized water, and dried in vacuum.

[0081] D...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for fixing polypeptide aptamers on the surface of a cardiovascular implanting device, which ensures that endothelial progenitor cells in the blood can be captured after the ardiovascular implanting device is implanted into a human body. The method comprises the steps of: surface activation, silanization treatment, biotin fixation, avidin fixation, albumin fixation, biotinylated aptamers fixation and the like. The method for fixing specific polypeptide aptamers for capturing the endothelial progenitor cells on the surface of the cardiovascular implanting device ensure that the cardiovascular implanting device can be used for capturing the endothelial progenitor cells in the blood after being implanted into a human body, and is favorable for inducing the rapid endothelium transition of the surface of the implanting device and improving the blood compatibility of a material, and thus, the implanting device has favorable functions of blood anticoagulation and restenosis inhibition.

Description

Technical field [0001] The invention relates to the surface modification technology of inorganic or metal materials, in particular to the surface modification technology of artificial organ materials, biological sensor materials, anti-corrosion materials and biological decontamination materials. Background technique [0002] Cardiovascular system disease is one of the important diseases that endanger human health. At the beginning of the 21st century, about 17 million people died of cardiovascular and cerebrovascular diseases in the world, and the death toll is expected to reach 25 million by 2020. At present, more than 2 million patients with coronary heart disease in the world need percutaneous puncture coronary angioplasty (PTCA) every year, but about 10% of patients who have implemented PTCA have acute or subacute coronary obstruction. The incidence of restenosis is as high as 20% to 30%. Restenosis has become a key problem of PTCA and the most important factor restricti...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): A61F2/82A61M31/00A61L31/16A61L33/12A61F2/95
Inventor 黄楠陈卓玥陈佳龙李全利王进冷永祥杨苹陈俊英孙鸿吴熹
Owner CO WITH LTD LIABILITY OF MEDICAL SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap