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Ceramide-containing tea seed extract and preparation method thereof

The technology of ceramide and extract is applied to the field of tea seed extract containing ceramide and its preparation, which can solve the problems of high cost and complicated process, and achieve the effects of low production cost, simple preparation process and easy availability of raw materials.

Active Publication Date: 2011-05-18
GUILIN NATURAL INGREDIENTS CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] There are many extraction methods for disclosed ceramides, such as extracting from rice (Agric.Biol.Chem., 49, 2753 (1985)) and wheat (Agric.Biol.Chem., 49, 3609 (1985)). Ceramide derivatives, using konjac as a raw material to extract and separate ceramides (Biotechnology, 2007, No. 10, Volume 17, No. 5, pages 45-47), and Japanese patent JP2004189683 also discloses ceramide-containing substances. Camellia plant extracts, but these extraction methods all have the disadvantages of complicated process and high cost

Method used

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  • Ceramide-containing tea seed extract and preparation method thereof
  • Ceramide-containing tea seed extract and preparation method thereof
  • Ceramide-containing tea seed extract and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1) Extraction and concentration: use the residue of camellia oleifera seeds as raw material after squeezing and extracting the oil and fat components, crush to 20 mesh, put into the extraction container, add 95% (v / v) ethanol equivalent to 3 times the weight of the raw material, and control the temperature at 80 DEG C, reflux extraction for 30 min for the first time, and separate the medicinal solution; add 95% (v / v) ethanol equivalent to 4 times the weight of the raw material for the second time, reflux extraction for 30 min at 80 DEG C, combine the extracts, reduce Press to recover ethanol to obtain extract;

[0027] 2) Hydrolysis: add sodium hydroxide solution equivalent to 5 times the weight of raw materials and a mass concentration of 10% to the extract for hydrolysis, the temperature of hydrolysis is 25 ° C, and the time is 15 hours;

[0028] 3) Extraction: the hydrolyzate is extracted twice with petroleum ether, and the extraction phases are combined; the amount ...

Embodiment 2

[0034] 1) Extraction and concentration: use the residue after extracting oil and fat from camellia seeds as raw material, crush it to 40 mesh, put it into an extraction container, add 90% (v / v) methanol equivalent to 5 times the weight of the raw material, and control the temperature at 60°C , stirring and extracting for 90 minutes, and separating the medicinal liquid; repeating the above conditions and then carrying out the second stirring and extraction; combining the extracts, recovering methanol under reduced pressure, and obtaining an extract;

[0035]2) Hydrolysis: Add potassium hydroxide solution equivalent to 5 times the weight of raw materials and a mass concentration of 10% to the extract for hydrolysis, the temperature of hydrolysis is 50 ° C, and the time is 3 hours;

[0036] 3) Extraction: the hydrolyzate is extracted twice with cyclohexane, and the extract phases are combined; the amount of cyclohexane used for extraction is 1 time of the volume of the hydrolyzate...

Embodiment 3

[0041] 1) Extraction and concentration: use tea tree seeds as raw materials, crush them to 80 mesh, put them into an extraction container, add water equivalent to 10 times the weight of raw materials, heat to 80°C for the first leaching, the time is 10 hours, and separate the drug liquid; for the second time, add water equivalent to 5 times the weight of the raw material, heat to 100 ° C for the second leaching, and the time is 3 hours, and separate the liquid medicine; add water equivalent to 1 time the weight of the raw material for the third time, Heating to 60°C for the third leaching for 1 hour, combining the extracts, concentrating under reduced pressure to obtain the extract;

[0042] 2) Hydrolysis: add sodium carbonate solution equivalent to 0.5 times the weight of raw materials and a mass concentration of 30% to the extract for hydrolysis, the temperature of hydrolysis is 80 ° C, and the time is 1 h;

[0043] 3) The obtained hydrolyzate is separated into the AB-8 type...

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Abstract

The invention discloses ceramide-containing tea seed extract and a preparation method thereof. The preparation method comprises the following steps of: crushing raw materials, extracting by using water or low-carbon alcohol, and recycling an extraction solvent from the obtained extracting solution so as to obtain extract, wherein the raw materials are seeds of theaceae camellia plants, namely camellia oleifera abel, camellia or common camellia, or residues of the seeds of the plants subjected to oil component extraction; adding alkaline solution into the extract, and hydrolyzing at the temperature of between 10 and 80 DEG C for 0.5 to 20 hours; and separating the obtained hydrolyzate by using a macroporous resin column, performing chromatography through a silica gel column or a sephadex column, eluting by using 40 to 60 volume percent aqueous solution of methanol or ethanol, performing thin layer chromatography, collecting fractions, mixing the fractions, and drying to obtain the ceramide-containing tea seed extract. The raw materials are readily available and the method is environmentally-friendly; the preparation process is simple; the product quality is stable; the production cost is low; and the content of ceramide in the extract is high.

Description

technical field [0001] The invention relates to the extraction of active ingredients in plants, in particular to tea seed extract containing ceramide and a preparation method thereof. Background technique [0002] Ceramide is composed of long-chain sphingosine covalently bonded to fatty acid through amide bond. Due to the difference in the carbon chain length of sphingosine in the ceramide molecule, the difference in the degree of unsaturation and hydroxylation, and the length of the fatty acid connected to the sphingosine through an amide bond, the number and position of unsaturated bonds and hydroxyl groups Different substances show great differences, so to be precise, ceramide is not a kind of substance, but a kind of substance with similar structure. Kristi JRobSon et al. have done detailed research on ceramides in the stratum corneum of the skin, and divided ceramides into seven types according to their structures. Ceramides are generally divided into Ceramide(I)-Cera...

Claims

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Application Information

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IPC IPC(8): A61K36/82A61P3/04A61P3/10A61P17/16A61P25/20A61P29/00A61P35/00A61K131/00
Inventor 孙步祥邱丽凰邓江胡厚才
Owner GUILIN NATURAL INGREDIENTS CORP
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