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Ginseng saponin H extract and preparation method thereof

A technology of ginsenosides and total ginsenosides is applied in the directions of drug combinations, pharmaceutical formulations, plant raw materials, etc., which can solve problems such as poor effect, and achieve the effects of novel use, high yield and convenient operation.

Inactive Publication Date: 2011-05-18
TIANJIN TASLY PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The effect of separating ginsenoside H in the prior art is not good. During the research process of the ginsenoside H extract, the inventor adopted a new separation technology to test out a group of new ginsenoside H extracts and their preparation. method, these ginsenoside H extracts have high curative effect, high purity, good absorption, stable quality, and at the same time the preparation method has good separation effect, high content, simple process, convenient operation, low cost, and is suitable for industrial production

Method used

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  • Ginseng saponin H extract and preparation method thereof
  • Ginseng saponin H extract and preparation method thereof
  • Ginseng saponin H extract and preparation method thereof

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Effect test

Embodiment 1

[0045] The preparation of embodiment 1 ginsenoside H extract

[0046] Step 1. American ginseng leaves were extracted with water twice, each time for 2 hours, adding 10 times the amount of water each time. After the extract was concentrated, it passed through a D101 macroporous adsorption resin column, and was first eluted with water until the effluent was nearly colorless, and then used 85% ethanol was eluted, and the 85% ethanol eluate was collected and concentrated to dryness to obtain total saponins;

[0047] Step 2. Dissolve the total saponins in 20 times the hydrochloric acid solution with a pH of about 1.0, and stir and react at 40°C for 4 hours. After the reaction is completed, adjust the pH to near neutral, precipitate out, collect the precipitate, and obtain the crude product of total ginseng glycosides;

[0048] Step 3, the crude product of total ginseng glycosides is dissolved by heating with 95% ethanol, mixed with 2 times the amount of silica gel, dried, crushed a...

Embodiment 2

[0049] The preparation of embodiment 2 ginsenoside H extract

[0050] Step 1. American ginseng leaves are extracted with water for 3 times, each time for 3 hours, adding 10 times of water each time, the extract is passed through a D101 macroporous adsorption resin column, and firstly eluted with water until the effluent is nearly colorless, then use 30% Eluted with ethanol, collected the eluate, concentrated to dryness to obtain total saponins;

[0051] Step 2. Dissolve the total saponins in 20 times the hydrochloric acid solution with a pH of about 1.0, and stir and react at 40°C for 4 hours. After the reaction is completed, adjust the pH to near neutral, precipitate out, collect the precipitate, and obtain the crude product of total ginseng glycosides;

[0052] Step 3. The crude product of total ginseng glycosides is dissolved by heating with 95% ethanol, mixed with 2 times the amount of silica gel, dried, crushed and sieved, and subjected to silica gel column chromatography...

Embodiment 3

[0053] The preparation of embodiment 3 ginsenoside H extract

[0054] Step 1. American ginseng is extracted with water, and the extract is passed through an AB-8 macroporous adsorption resin column, and firstly eluted with water until the effluent is nearly colorless, followed by elution with 95% ethanol, and the 95% ethanol eluate is collected. Concentrate to dryness to obtain total saponins;

[0055] Step 2. Dissolve the total saponins in 20 times the sulfuric acid solution with a pH of about 1.0, stir and react at 40°C for 4 hours, adjust the pH to near neutral after the reaction, precipitate and collect the precipitate to obtain the crude product of total ginsenosides;

[0056] Step 3. The crude product of total ginseng glycosides was dissolved by heating with 70% ethanol, mixed with 2 times the amount of silica gel, dried, crushed and sieved, subjected to silica gel column chromatography, and eluted with chloroform-methanol (2:1) system. Thin-layer TLC checks the elution...

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Abstract

The invention relates to a traditional Chinese medicine extract and a preparation method thereof, in particular to a Ginseng saponin H extract and a preparation method thereof. In the extract, the content of protodioscin is not less than 60 percent based on that of Ginseng saponin Rh2 and the content of the Ginseng saponin Rh1 and the Ginseng saponin Rh2 is not less than 30 percent.

Description

technical field [0001] The invention relates to a traditional Chinese medicine extract and a preparation method thereof, in particular to a ginsenoside H extract and its preparation. Background technique [0002] Ginseng has many reports in the treatment of cardiovascular and cerebrovascular diseases. [0003] Ginsenoside can treat coronary heart disease and ischemic stroke. [0004] Ginsenoside H is the secondary saponin produced by hydrolysis of ginsenoside components, and then purified by silica gel column chromatography to obtain the effective part-H group saponin. The main ingredient of the drug is also the main anti-cancer active ingredient of the drug. Pharmacodynamic studies have shown that: ginsenoside Rh2 has a significant and specific inhibitory effect on the proliferation of cancer cells such as liver cancer, melanoma B16, cervical cancer and sarcoma S180. Its mechanism of action is as follows: in the process of cancer cell proliferation, through the action of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/258G01N21/31G01N30/02A61P35/00A61K31/704A61K125/00A61K127/00
Inventor 张兰兰宋兆辉林万里蔡楠黄芝娟周水平朱永宏
Owner TIANJIN TASLY PHARMA CO LTD
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