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Large-scale culture method of cordyceps sinensis

A technology of tissue culture of Cordyceps militaris, which is applied in the field of large-scale cultivation of Cordyceps militaris, can solve the problems of not being able to meet the normal growth requirements of Cordyceps militaris, low biotransformation rate of Cordyceps militaris, uneven light intensity, etc., so as to improve weed yield or biotransformation rate , avoid the effect of incomplete differentiation of primordium and sufficient light

Inactive Publication Date: 2011-05-04
SHANGHAI GUOBAO ENTERPRISE DEV CENT +1
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Problems solved by technology

[0009] The purpose of the present invention is to propose a method for large-scale cultivation of Cordyceps militaris. The method of the present invention provides suitable light intensity and light intensity for growth according to the growth characteristics of each period of Cordyceps militaris fruiting body cultivation by adjusting light intensity and light cycle. Photoperiod, to meet its growth needs, to solve the current large-scale cultivation of Cordyceps militaris due to uneven light intensity and no controllable light can not meet the normal growth needs of Cordyceps militaris, resulting in low rate of Cordyceps militaris, poor grass shape, A series of problems such as low content of active ingredients in the fruit body and low biotransformation rate of Cordyceps

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Embodiment 1

[0055] In this embodiment, a total of 89 groups were cultured, each group having 100 culture containers, and the Cordyceps militaris was cultured by conventional tissue culture methods and medium. The ambient temperature and humidity are controlled within the range of ambient temperature and humidity described in the method of the present invention. Other parameters of culture conditions were selected according to routine.

[0056] During the hyphae color change period, according to light intensity 100lux, 200lux, 300lux and photoperiod 12 / 12 (light / dark, h), 24 / 0 (light / dark, h), 18 / 6 (light / dark, h) ), two conditions and three factors were carried out for orthogonal experimental cultivation, and the experimental results were obtained: the shortest color change period was 2 days under the conditions of light intensity of 200lux and light cycle of 24 / 0 (light / dark, h). The experimental conditions are: ambient temperature: 20°C-21°C, ambient relative humidity: 60%-70%.

[005...

Embodiment 2

[0062] In this embodiment, 10 groups were cultured, each group had 100 culture containers, and conventional tissue culture methods and medium were used to cultivate Cordyceps militaris.

[0063] The light-shielded culture step is to cultivate in a light-shielded environment for 4-5 days; the ambient temperature of the light-shielded culture step is controlled at 20-21° C., and the relative humidity of the environment is controlled at 60%-70%.

[0064] The illuminance, photoperiod, temperature and humidity of the light cultivation step are as follows:

[0065] Mycelia color change period: the light intensity is controlled to 200lux, and the light cycle is controlled to be 24 / 0 (light / dark, h); the ambient temperature is controlled to be 20-22°C, and the relative humidity of the environment is controlled to be 60%-70%;

[0066]Primordia differentiation and formation period of fruiting body growth period: light intensity control is 650lux, light cycle control is 9 / 15 (light / dark,...

Embodiment 3

[0076] In this embodiment, 10 groups were cultivated, each group had 100 culture containers, and the conventional tissue culture method was adopted to cultivate Cordyceps militaris in the culture medium.

[0077] In the step of cultivating in the dark, the culture is carried out in a dark environment for 4-5 days; the ambient temperature of the dark cultivating step is controlled at 20-21° C., and the relative humidity of the environment is controlled at 60-70%.

[0078] The illuminance, photoperiod, and temperature and humidity of the light cultivation step are as follows:

[0079] Mycelia color change period: the light intensity is controlled to 100lux, and the light cycle is controlled to be 24 / 0 (light / dark, h); the ambient temperature is controlled to be 20-22°C, and the relative humidity of the environment is controlled to be 60%-70%;

[0080] Primordia differentiation formation period of fruiting body growth period: light intensity control is 200lux, light cycle control...

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Abstract

The invention relates to a culture method of cordyceps sinensis, in particular to a large-scale culture method of cordyceps sinensis. The method comprises the following steps: after inoculations, culture vessels are arranged and stacked in tissue culture rooms on a controllable optical culture shelf; the culture process contains the steps of non-illuminating culture and illuminating culture; and in the illuminating culture step, the controllable optical culture shelf can be utilized to adjust the illumination intensity and the illumination period. By adopting the method in the invention, a serie of problems of the existing large-scale culture method of cordyceps sinensis that as the illumination intensity is not uniform and the illumination is not controllable, the normal growth demand of cordyceps sinensis can not be satisfied, the grass yield of cordyceps sinensis is low, the grass shape is poor, the contents of active ingredients in a fruiting body are low, the biological conversion rate of cordyceps sinensis is low and the like can be solved. In addition, in the method in the invention, the illumination intensity range and illumination period of the grass-growing management stage of the cordyceps sinensis culture can be adjusted to increase the contents of active ingredients in the fruiting body and the accumulation of biomass in the fruiting body.

Description

technical field [0001] The invention relates to a method for cultivating Cordyceps militaris, in particular to a method for cultivating Cordyceps militaris on a large scale. Background technique [0002] The date of authorization announcement is CN1157472C, and the Chinese invention patent specification whose authorization announcement date is July 14, 2004 discloses a method for cultivating fruiting bodies of Cordyceps militaris. The method for cultivating fruit bodies of Cordyceps militaris comprises the steps of 1) preparation of rice culture medium, 2) inoculation of hyphae, 3) dark culture, 4) light culture, and 5) harvest of fruit bodies. Wherein said step 1) preparation of rice culture medium: the unit is g / ml, rice is added to nutrient water, the weight ratio of rice and nutrient water is 1:1~1:2; Potassium dihydrogen 0.3%, magnesium sulfate heptahydrate 0.02%, casein 0.5%, selenium-enriched yeast 0.01-0.1%, the pH of the nutrient water is 6.0-7.5; the rice medium i...

Claims

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Application Information

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IPC IPC(8): A01G1/04A01G9/20
Inventor 唐永范唐亮
Owner SHANGHAI GUOBAO ENTERPRISE DEV CENT
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