Method for detecting musk ketone

Abstract

The invention belongs to the field of drug detection, relates to a method for detecting musk ketone and aims at providing the method for detecting the musk ketone, wherein the method is simple to operate, strong in specificity, stable and good in repeatability. The detection method adopts the high performance liquid chromatography for determination, and the detection condition and the method are as follows: the chromatographic condition is that octadecylsilane chemically bonded silica is taken as a filling agent; methanol-water (90-95): (5-10) or acetonitrile-water (90-95): (5-10) is adopted as a mobile phase; and the detection wavelength is 283nm. Control solution is prepared as follows: precisely weighing an appropriate amount of musk ketone control, dissolving the musk ketone in acetonitrile or methanol, and preparing test solution by taking a musk or musk-containing preparation as a detection object. The control solution and the test solution are respectively precisely sucked and injected into a liquid chromatographic instrument for determination. The detection method does not need to carry out pre-column derivatization, the sample preparation is simple, the requirements on equipment conditions are not high, the specificity is strong, the stability and the repeatability are good, and the operation is convenient.

Description

technical field [0001] The invention belongs to the field of drug detection and relates to a detection method of muscone. Background technique [0002] Musk is a precious spice and traditional Chinese medicine, and its aroma is mainly derived from the muscone contained therein. Pharmacological studies have shown that muscone is an important physiologically active component in natural musk, and the detection of this component is one of the important indicators for evaluating the quality of musk medicinal materials. [0003] Existing muscone detection methods mainly include gas chromatography, thin-layer chromatography, pre-column derivatization high-performance liquid chromatography, high-performance liquid chromatography-mass spectrometry and colorimetry. [0004] The application of gas chromatography to detect muscone is the method adopted in the 2005 edition of the Chinese Pharmacopoeia. The accuracy of its analysis is unquestionable. However, due to the use of high press...

AI Technical Summary

Problems solved by technology

[0005] When thin-layer chromatography is used to detect muskone, because the method uses manual preparation of thin-layer plates, a relatively open development method and color development method, the qualitative detection is acceptable, but the reproducibility of quantitative detection is poor.

[0006] Application of pre-column derivatization high-performance liquid chromatography to detect muskone, that is, before sample analysis, the sample and reference substance are reacted with 2,4-dinitrophenylhydrazine to generate phenylhydrazone, and the absorption peak of phenylhydrazone is used for detection. cumbersome operation

[0007] Application of high performance liquid chromatography-mass spectrometry to detect muscone, this method is a better detection method, but the instrument is expensive and not easy to popularize

[0008] The colorimetric method is used to detect muskone, because the method must first separate and purify the sample and then develop the color measurement, the operation is more cumbersome

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