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Duck infectious serositis inactivated vaccine emulsification method

An inactivated vaccine and infectious technology, which is applied in the emulsification field of duck infectious serositis inactivated vaccine, can solve the problems of vaccine quality impact, emulsification production process without formal procedures, and the safety of inoculated animals, etc., to achieve stability Good, the qualified rate of dosage form is improved, and the effect of uniform color and luster

Active Publication Date: 2011-03-30
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the inactivated vaccine is really effective. As far as the current research progress is concerned, it is the best choice for the prevention of Riemerella anatipestifer. There are no formal procedures for the formula and emulsification process of inactivated vaccines, but the formula and emulsification process of inactivated vaccines are very important to the quality of vaccines. They not only affect the safety of vaccinated animals, but also have a direct impact on the quality of vaccines. It is one of the foundations and keys for the production of R. anatipestifer inactivated vaccines. Currently, there is no report on the effect of the formulation and emulsification process for industrialized large-scale fermentation of R. anatipestifer inactivated vaccines.

Method used

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  • Duck infectious serositis inactivated vaccine emulsification method
  • Duck infectious serositis inactivated vaccine emulsification method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Preparation of 1% lysed hemocyte whole blood N-J synthetic medium:

[0017] Prepare 100ml of 1% lysed blood cell whole blood N-J synthetic medium: tryptone: 1.9g; yeast extract: 0.7g; beef extract: 0.7g; hydrolyzed milk protein: 0.7g; glucose: 0.4g; NaCl: 0.5g ; Potassium dihydrogen phosphate: 0.3g; Dipotassium hydrogen phosphate: 0.3g; Na 2 HPO 4 12H 2 O: 0.9g; (NH 4 ) 2 SO 4 : 0.2g; NH 4 Cl: 0.03g; add water to 100mL; sterilize the above-mentioned components at 121°C for 15 minutes, and when the sterilized components are cooled to below 40°C, add 1ml of lysed blood cells and whole blood to the above-mentioned basal medium; After shaking well, the finished N-J synthetic medium can be obtained.

[0018] The whole blood of the added lysed hemocytes is aseptically collected from healthy bovine blood in non-epidemic areas by jugular vein blood collection or carotid artery bloodletting and aseptically defibrillated, frozen and thawed at -20°C and room temperature, an...

Embodiment 2

[0020] Preparation of duck infectious serositis inactivated vaccine vaccine preparation liquid:

[0021] 1 strain: Serum Type I Riemerella anatipestifer RA-CH-I strain.

[0022] 2 Culture medium: 1% lysed blood cell whole blood N-J synthetic medium, 10% serum nutrient agar plate, lysed whole blood and inspection medium are all produced in strict accordance with the appendix of "Chinese Veterinary Pharmacopoeia" and tested after passing the test.

[0023] 3 methods

[0024] 3.1 Propagation and identification of first-class seeds

[0025] Put the freeze-dried basal strain of serotype I Riemerella anatipestifer RA-CH-I into 5ml of nutrient broth containing 10% calf serum, and culture it at 37°C for 24 hours; then streak inoculated in 2 On the nutrient agar plate containing 10% calf serum, according to the standard [smooth surface, slightly protruding, round, creamy colonies, colony diameter 1.2 ~ 1.7mm; bacterial smears can be seen as single (occasionally in pairs) or filament...

Embodiment 3

[0039] Screening of the best emulsification conditions:

[0040] Material:

[0041] 1.1 Bacterial liquid: Riemerella anatipestifer RA-CH-I strain is used for seedling preparation.

[0042] 1.2 White oil for injection was purchased from Hangzhou Oil Refinery.

[0043] 1.3 Siben-80 was purchased from Chengdu Dongjin Chemical Reagent Factory.

[0044]1.4 Aluminum stearate was purchased from Shanghai Yuanhang Chemical Reagent Factory.

[0045] 1.5 Tween-80 was purchased from Chengdu Dongjin Chemical Reagent Factory.

[0046] 1.5 Formaldehyde solution AR was purchased from Chengdu Honghe Reagent Factory.

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Abstract

The invention discloses a duck infectious serositis inactivated vaccine emulsification method which comprises the following steps of: water phase preparation: loading four parts of Tween-80 into a bottle to be sterilized, adding 96 parts of germ liquid after cooling and sufficiently vibrating and shaking until the Tween-80 is completely dissolved; oil phase preparation: adopting 93 parts of white oil for injection, 5 parts of spun-80 and 2 parts of aluminum stearate, firstly taking a small amount of white oil for injection to be mixed with the aluminum stearate while preparing the oil phase, heating and dissolving the mixture, then, uniformly mixing the total amount of spun-80 with the white oil for injection, stirring the mixture until in the transparent state, and sterilizing for 30 minutes at 116 DEG C for use; and emulsifying the water phase and the oil phase according to a proportion of 1:1.5 to 1:1.3 at the rotating speed of 3200 to 4800 r / min for 8 to 12 minutes.

Description

technical field [0001] The invention relates to the technical field of veterinary biological preparations, in particular to an emulsification method of duck infectious serositis inactivated vaccine. Background technique [0002] Duck infectious serositis, also known as new duck disease, duck septicemia, duck plague syndrome, pasteurella anatipestifer, etc., is a contact infection caused by Riemerella anatipestifer (RA) Diseases are more common in ducklings aged 1 to 8 weeks, especially ducklings aged 2 to 5 weeks are most susceptible. The incubation period is generally 2 to 5 days, and it is acute or chronic sepsis. Clinically, it is mainly manifested as increased eye and nasal secretions, panting, coughing, diarrhea, ataxia, and head and neck tremor. A small number of chronic cases have symptoms such as head and neck distortion. The lesions are characterized by fibrinous pericarditis, perihepatitis, air sac inflammation, meningitis and arthritis in some cases, which often ...

Claims

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Application Information

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IPC IPC(8): A61K39/02A61K9/107A61K47/44A61P31/04A61K47/06A61K47/12A61K47/26
Inventor 程安春汪铭书朱德康陈孝跃
Owner SICHUAN AGRI UNIV
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