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Method for biosynthesizing astaxanthin by using exponential intermittent feed supplement mode

A biosynthesis and astaxanthin technology, applied in the field of bioengineering, can solve the problems of low natural astaxanthin output, competition, and high production costs, and achieve high promotion and application value, increase output, and simple operation

Inactive Publication Date: 2011-03-16
DALIAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As far as the current production and market situation is concerned, it is difficult for natural astaxanthin to compete with chemically synthesized astaxanthin in terms of price due to low yield and high production cost

Method used

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  • Method for biosynthesizing astaxanthin by using exponential intermittent feed supplement mode

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A. Selection of raw materials: select fresh and ripe tomatoes containing astaxanthin synthesis precursors, wash, cut into pieces, squeeze the juice, and filter. The filtrate was sterilized at 121°C for 20 minutes, and stored at 4°C for later use.

[0029] B. Activation of strains: The strains preserved on the slant were activated and cultivated on the YM slant medium at 23°C, and cultured in the YM medium at 23°C with liquid ventilation.

[0030] C. Liquid culture: the YM seed liquid was cultured at 23° C. for 36 hours in a liquid medium with an inoculum amount of 8%.

[0031] D. Fed-batch feeding of precursor-rich additive solution: After 36 hours of fermentation, 1% tomato juice was added every 12 hours. After two consecutive additions, feed feeding was stopped and culture continued for 36 hours.

[0032] E. Extraction of astaxanthin: determination of astaxanthin content in Phaffia rhodozyme.

[0033] The resulting cyanin content was increased by 91% compared with t...

Embodiment 2

[0035] Get carrot and implement by A, B steps in example 1.

[0036] C. Liquid culture: the YM seed liquid was cultured at 22° C. for 24 hours in a liquid medium with an inoculum amount of 10%.

[0037] D. Fed-batch feeding of precursor-rich additive solution: After 24 hours of fermentation, 0.5% carrot juice was added every 6 hours. After three consecutive additions, feed feeding was stopped and culture continued for 24 hours.

[0038] E. Extraction of astaxanthin: determination of astaxanthin content in Phaffia rhodozyme.

[0039] The resulting cyanin content was increased by 27% compared with the control group.

Embodiment 3

[0041] A. Take corn flour for gelatinization, then sterilize at 121°C for 20 minutes, and store it at 4°C for later use.

[0042] B. Activation of strains: the strains preserved on the slant were activated and cultivated on the YM slant medium at 24°C, and cultured in the YM medium at 24°C with liquid ventilation.

[0043] C. Liquid culture: the YM seed liquid was cultured at 24° C. for 48 hours in a liquid medium with an inoculum amount of 12%.

[0044] D. Feed-feeding of precursor-rich additive solution: After 48 hours of fermentation, 2% corn steep liquor was added every 3 hours. After 4 consecutive additions, feed-feeding was stopped and culture continued for 48 hours.

[0045] E. Extraction of astaxanthin: determination of astaxanthin content in Phaffia rhodozyme.

[0046] The resulting cyanin content was increased by 47% compared with the control group.

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Abstract

The invention relates to a method for biosynthesizing astaxanthin by using an exponential intermittent feed supplement mode. The method comprises the following steps: S1, taking subsidiary agricultural products rich in synthetic precursors of the astaxanthin to prepare an adding fluid and sterilizing the adding fluid for 15-30 minutes at the temperature of 115-121 DEG C; S2, taking and cultivating Phaffia rhodozyma in a liquid fermentation medium, feeding 0.5-5% the adding fluid in a batch mode at intervals of 3-12 hours after the exponential phase, and after carrying out fed-batch for 1-4 times, continuing to carry out cultivation 12-48 hours; and S3, extracting the astaxanthin. In the method of the invention, a small amount of astaxanthin synthetic accelerator is added in the exponential phase of the fermentation, thus avoiding premature addition of the astaxanthin synthetic accelerator from inhibiting yeast plant growth and greatly improving the yield of the astaxanthin at the same time, wherein the yield of the astaxanthin is improved by 20-95% compared with a control group. The extracted astaxanthin can be used as feed additives in high-grade aquatic products industry, fish breeding and poultry raising industry and the like. The invention has the advantages that the raw material price is low, the operation is simple and the popularization and application value is high.

Description

technical field [0001] The invention relates to the technical field related to bioengineering, in particular to a method for producing astaxanthin by fermenting raw materials of agricultural and sideline products rich in astaxanthin precursors. Background technique [0002] Astaxanthin is 3,3'-dihydroxy-4,4'-diketone-β,β'carotene, which is a ketone carotenoid. The color is bright red and fat-soluble. Because of its special coloring function, it can color the meat of fish, shrimp shell, poultry skin and yolk, making it bright in color, so it can be used as a feed nutrient enhancer for high-grade aquaculture poultry; in addition, astaxanthin can also It has many other physiological functions, such as highly quenching monoatomic oxygen and scavenging free radicals; anti-oxidation, its ability to resist fat oxidation is 10 times higher than β-carotene, 100 times higher than vitamins, and can effectively prevent organization. Cells and DNA are oxidatively damaged, enhance immu...

Claims

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Application Information

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IPC IPC(8): C12P23/00C12R1/645
Inventor 王际辉侯娟叶淑红王晗
Owner DALIAN POLYTECHNIC UNIVERSITY
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