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Biological model of steroids hormone pollutant in foods and feeds established by utilizing Caenorhadits elegans and method thereof

A technology of Caenorhabditis elegans and biological models, applied in animal husbandry and other fields, can solve the problems of long cycle, high cost, and high technical requirements for experimenters, and achieve the effects of low cost, intuitive observation, and high sensitivity

Inactive Publication Date: 2011-03-02
CHANGCHUN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the research methods of steroid hormones in food and feed at home and abroad mainly include high performance liquid chromatography, radioimmunoassay, spectrophotometry, thin layer chromatography, gas chromatography-mass spectrometry (GC-MS) and other chemical methods. Methods and biological methods, these methods are to study steroid hormones from a quantitative point of view, and the cost is high, the cycle is long, and the technical requirements for the experimenters are also high.

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0044] Embodiment 1: establish estradiol pure product biological model with Caenorhabditis elegans

[0045] 1. Obtain the first phase (L1 phase) worm fluid of synchronized Caenorhabditis elegans larvae:

[0046] Wash the adult C. elegans hermaphrodites in the egg-laying stage from the 90 mm NGM culture plate with M9 buffer, centrifuge at 1500 rpm for 1 min, and discard the supernatant. After washing once with M9 buffer as above, dilute the volume of M9 buffer containing Caenorhabditis elegans to 500 μl in a 1.5ml EP tube. Add 500 μl of lysate, mix up and down for 3-5 minutes, then centrifuge at 1500 rpm for 3 minutes, discard the supernatant, and collect the bottom precipitate. The precipitate was washed repeatedly with sterile S-Medium for 2-3 times until the pH value reached 7.0. At this time, the body of Caenorhabditis elegans had been lysed and the eggs were still intact. Place the sterile S-Medium solution containing Caenorhabditis elegans eggs at 20°C for 8-12h. Since...

Embodiment 2

[0063] Embodiment 2: Establish steroid hormone biological model in pork with Caenorhabditis elegans

[0064] 1. Obtain the first phase (L1 phase) worm fluid of synchronized Caenorhabditis elegans larvae:

[0065] Wash the adult C. elegans hermaphrodites in the egg-laying stage from the 90mm NGM plate with M9 buffer, centrifuge at 1500rpm for 1min, and discard the supernatant. After washing once with M9 buffer as above, dilute the volume of M9 buffer containing Caenorhabditis elegans to 500 μl in a 1.5ml EP tube. Add 500 μl of lysate, mix up and down for 3-5 minutes, then centrifuge at 1500 rpm for 3 minutes, discard the supernatant, and collect the bottom precipitate. The precipitate was washed repeatedly with sterile S-Medium for 2-3 times until the pH value reached 7.0. At this time, the body of Caenorhabditis elegans had been lysed and the eggs were still intact. Place the sterile S-Medium solution containing Caenorhabditis elegans eggs at 20°C for 8-12h. Because there i...

Embodiment 3

[0082] Embodiment 3: Establish steroid hormone biological model in milk powder with Caenorhabditis elegans

[0083] 1. Obtain the first phase (L1 phase) worm fluid of synchronized Caenorhabditis elegans larvae:

[0084] Wash the adult C. elegans hermaphrodites in the egg-laying stage from the 90 mm NGM culture plate with M9 buffer, centrifuge at 1500 rpm for 1 min, and discard the supernatant. After washing once with M9 buffer as above, dilute the volume of M9 buffer containing Caenorhabditis elegans to 500 μl in a 1.5ml EP tube. Add 500 μl of lysate, mix up and down for 3-5 minutes, then centrifuge at 1500 rpm for 3 minutes, discard the supernatant, and collect the bottom precipitate. The precipitate was washed repeatedly with sterile S-Medium for 2-3 times until the pH value reached 7.0. At this time, the body of Caenorhabditis elegans had been lysed and the eggs were still intact. Place the sterile S-Medium solution containing Caenorhabditis elegans eggs at 20°C for 8-12h...

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Abstract

The invention relates to a biological model of steroids hormone pollutant in foods and feeds established by utilizing Caenorhadits elegans and a method thereof, belonging to the field of a safety detection technique of foods and feeds. The biological model of the steroids hormone pollutant in the foods and the feeds is established by utilizing the Caenorhadits elegans. The method of the invention is characterized in that the Caenorhadits elegans is utilized to establish a biological model of steroids hormone pure products; the Caenorhadits elegans is utilized to establish the biological model of steroids hormone in the foods; and the Caenorhadits elegans is utilized to establish a biological model of steroids hormone in the feeds. The invention has the beneficial effects of important significance of the research of the steroids hormone to influence on human health, and short period, high efficiency, conciseness and convenience of the experiment course, less requirements on instruments, low cost and great application value. Moreover, the period of the Caenorhadits elegans from the first stage of larvae to the stage of oviposition and the generation time are detected to identify the precocity phenomenon of organisms under the influence of the steroids hormone by exposing the steroids hormone of the Caenorhadits elegans in the first stage of the larvae.

Description

technical field [0001] The invention belongs to the technical fields of food safety detection, feed safety detection and the like. Background technique [0002] Caenorhabditis elegans (C.elegans) is a kind of nematode that can survive independently. Its individual is small, the adult is only 1.5mm long, it is hermaphroditic, male individuals only account for 0.2% of the population, and can be self-fertilized or bisexual reproduction , the average life cycle is 3.5 days at 20°C, and the average fecundity is 300-350, but if it mates with males, it can produce more than 1400 offspring. The genome size of Caenorhabditis elegans is 97Mb, which is distributed on 6 chromosomes. It is predicted that there are 19,717 coding genes, and 40% of the genome sequence is homologous to humans, which means that it shares some similar cellular and molecular structures with higher organisms and Control pathway; it is the first multicellular eukaryote whose gene body has been completely sequenc...

Claims

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Application Information

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IPC IPC(8): A01K67/033
Inventor 于源华张昊李金海于化东张淑华姚健宋禹杨佳新林铭燕徐任
Owner CHANGCHUN UNIV OF SCI & TECH
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