Method for preparing chymosin by mixed culture fermentation of bean dregs and waste material from fungal culture (WMFC)

Abstract

The invention provides a method for preparing chymosin by mixed culture fermentation of bean dregs and waste material from fungal culture (WMFC). The method comprises the steps of: adding the WMFC into wet bean dregs, inoculating a tiny mucor strain for solid fermentation, and extracting the chymosin from a fermentation product. In the method, the respective characteristics of the bean dregs and the WMFC are skillfully utilized, sundried WMFC from edible fungi and the wet bean dregs are mixed to adjust the proportion of water content to dry materials in a solid fermentation medium, and thus, the wet bean dregs can be prevented from caking due to overhigh water content, a link of drying the bean dregs is omitted, and enzyme activity obtained through fermentation cannot be lowered due to the doping of the WMFC from the edible fungi. Above all, by mixing the wet bean dregs and the dry WMFC from the edible fungi to replace the wheat bran to carry out solid fermentation on the chymosin, wastes are turned into wealth, the production cost of the chymosin is reduced, and the technology is simple, convenient and easy to operate, is time-saving and economical and has higher enzyme activity.

Description

technical field [0001] The invention relates to a method for preparing microbial chymosin, more specifically to a method for preparing rennet by mixed fermentation of bean dregs and edible fungus chaff, and belongs to the technical field of producing rennet by solid fermentation using waste resources. Background technique [0002] Okara is the main by-product of soybean processing. It has been treated as low-value-added feed or fertilizer for many years, and some are even discarded directly. It not only has low economic benefits, wastes resources, but also causes environmental pollution. The comprehensive utilization and processing of bean dregs by means of modern biotechnology such as fermentation technology and enzyme technology can fully develop its nutritional components, solve the environmental pollution caused by discarded bean dregs, and realize the recycling of waste, which has become a hot spot and trend of current research. . [0003] Mushroom bran is the use of s...

AI Technical Summary

Problems solved by technology

Although domestic scholars such as Lu Fei have reported using Actinomucor elegans for solid-state fermentation of bean dregs, and its proteolytic enzyme activity can reach 600U / g, but their research did not involve Mucor pusillus, and the study only Limited to proteolytic enzymes, not involved in the production of chymosin by solid fermentation

The technology of producing rennet by solid fermentation by cultivating Mucor pusillus with the mixture of wet bean dregs and dry edible fungus chaff has not been reported yet.