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Processing method for mussel isolated protein

A protein separation and processing method technology, applied in the field of protein extraction, can solve the problems of protein powder insoluble in water, low protein purity, low purification efficiency, etc., to achieve improved protein extraction rate, high protein extraction rate, and high purity Effect

Inactive Publication Date: 2011-02-09
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] 3) Adding acid to precipitate protein at the isoelectric point is simple and low cost, but co-precipitation often occurs, the purification efficiency is not high, and the purity of the precipitated protein is not high;
[0012] 4) The final precipitated protein is freeze-dried to obtain protein powder. Although freeze-drying can theoretically maintain the original structure and activity of the protein, in fact, the protein powder obtained in this way is difficult to dissolve in water, even under stirring. ;

Method used

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  • Processing method for mussel isolated protein

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Effect test

Embodiment 1

[0029] Embodiment 1, a kind of processing method of mussel protein isolate, carries out following steps successively:

[0030] 1), the mussel meat was chopped, freeze-dried (-60°C, 24 hours), crushed to 60 mesh, CO 2 Supercritical extraction and degreasing (fat is used for other purposes) to obtain defatted mussel powder with a moisture content of ≤2.5%.

[0031] CO 2 The process conditions of supercritical extraction degreasing can be, for example:

[0032] The thickness of the material (crushed mussel meat) is 5-10mm, the freezing time is 5 hours, the freezing end temperature is -25°C, the cold trap temperature is 50-60°C, the vacuum degree is 25-50pa, the final freeze-drying temperature is 25°C, and the drying time is 20h . The pressure of the separation tank I is 7-8MPa and the temperature is 60°C; the pressure of the separation tank II is 4-6MPa and the temperature is 35°C; the pressure of the extraction tank is 30MPa and the temperature is 45°C; CO 2 The flow rate is...

Embodiment 2

[0040] Embodiment 2, a kind of processing method of mussel protein isolate, carries out following steps successively:

[0041] 1), the mussel meat is chopped, freeze-dried, crushed to 50 mesh, CO 2 Supercritical extraction and degreasing to prepare defatted mussel powder with a moisture content of ≤2.5%. The defatted mussel powder is detected by the Kjeldahl method, and the protein content is 45%.

[0042] 2), above-mentioned 1.5 kilograms of defatted mussel powders are mixed with 45L water, adjust pH to 10 with NAOH solution, soak under stirring condition after 2 hours, centrifuge 15min at 10000rmp, obtain filtrate I and filter residue.

[0043] 3) According to the dosage ratio of 1 kg of filter residue to 2L of water, add water to the above filter residue, soak for 0.5 hour under stirring condition, and centrifuge at 10000rmp for 15min to obtain filtrate II and sediment.

[0044] 4), the above-mentioned filtrate I and filtrate II were combined, and the pH was adjusted to n...

Embodiment 3

[0048] Embodiment 3, a kind of processing method of mussel protein isolate, carries out following steps successively:

[0049] 1), the mussel meat is chopped, freeze-dried, crushed to 40 mesh, CO 2 Degrease by supercritical extraction to make defatted mussel powder. The above-mentioned defatted mussel powder is detected by the Kjeldahl method, and the protein content is 44.5%.

[0050] 2) Mix 1 kg of the defatted mussel powder with 20 L of water, and adjust the pH to 11.5 with NAOH solution. After soaking for 1.5 hours under stirring conditions, centrifuge at 10000rmp for 15min to obtain filtrate I and filter residue.

[0051] 3) According to the dosage ratio of 1 kg of filter residue to 2.9 L of water, add water to the above filter residue, soak for 0.5 hour under stirring conditions, and centrifuge at 10,000 rpm for 15 minutes to obtain filtrate II and sediment.

[0052] 4), the above-mentioned filtrate I and filtrate II were combined, and the pH was adjusted to neutral w...

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Abstract

The invention discloses a processing method for mussel isolated protein, which sequentially comprises the following steps of: 1) cutting mussel meat into pieces, performing freeze drying, crushing, and defatting to obtain defatted mussel powder; 2) mixing the defatted mussel powder and water, adjusting pH to be between 10 and 12, soaking, and centrifuging to obtain filtrate I and filter residue; 3) soaking the filter residue in water, and centrifuging to obtain filtrate II and sediment; 4) combining the filtrate I and the filtrate II, and adjusting the pH to neutral to obtain total filtrate; and 5) concentrating and drying the total filtrate to obtain the mussel isolated protein. The mussel isolated protein prepared by the method has the characteristics of good solubility and high protein content.

Description

technical field [0001] The invention relates to the extraction of protein, in particular to the extraction of mussel protein isolate and its processing technology. Background technique [0002] Mussels are common marine shellfish and seafood treasures. Its meat is delicious and nutritious, especially the protein content is as high as 47.8%. It contains all 8 essential amino acids for the human body, and the content of essential amino acids is much higher than that of eggs and chickens. , duck, fish, shrimp and meat and other food. The amino acid content of mussel protein is shown in Table 1. [0003] Table 1. Nutrient composition list of mussels (dry) [mussels, shell vegetables] (contained in every 100 grams) [0004] [0005] At present, the protein isolates on the market are mainly vegetable protein powder, and there are few reports on seafood protein. With the expansion of the mussel culture area in my country, the mussel production is increasing rapidly. Because mu...

Claims

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Application Information

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IPC IPC(8): A23J1/04
Inventor 李铎张旭马留斯
Owner ZHEJIANG UNIV
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