Biogenic pesticide for efficiently preventing and controlling main diseases of Camellia oleifera and application thereof
A bio-derived pesticide, camellia oleifera technology, applied in the application, biocide, chemicals for biological control, etc., can solve the problems of unfavorable sustainable development of camellia oleifera industry, endangering human health, affecting the quality of camellia oil, etc. Promote the growth of Camellia oleifera, reduce the possibility of resistance, and have good control effects
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Embodiment 1
[0018] Screening of plant extracts for effective control of major diseases of camellia oleifera
[0019] Using mycelia growth rate method: respectively draw 1ml of the plant extract solution with a concentration of 20mg / ml described in Table 1, and pour it into a sterilized Petri dish containing 9ml of PDA, and use 1ml of sterile water for the control, and wait for the culture medium After solidification, use a puncher with a diameter of 0.8 cm to make a fungus dish, use an inoculation needle to inoculate the fungus dish to the center of the culture medium, with the mycelium facing down, one piece for each dish, repeat 3 times for each treatment, and culture in a 28 incubator , and the colony diameter was measured by the cross method after 4 days of culture. Inhibition rates were calculated as the average of three replicates. The results are shown in Table 1
[0020] Table 1 Inhibitory effect of different plant extracts on major diseases of camellia oleifera
[0021]
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Embodiment 2
[0024] Effects of Biogenic Pesticides with Different Volume Ratio on the Growth of Camellia oleifera
[0025] Select 2-year-old Camellia oleifera seedlings with the same growth, and measure the height of each seedling, spray different proportions of biological source pesticides on the seedlings, and use sterile water as a control, spray 20 seedlings for each treatment, and spray again after 7 days. Measure the height of the seedlings one month later. The influence of biogenic pesticides on the growth of camellia oleifera was measured, and the results are shown in Table 2. Biogenic pesticides with different volume ratios can all promote the growth of camellia oleifera to a certain extent.
[0026] Table 2 Effects of different volume ratios of biogenic pesticides on the growth of Camellia oleifera
[0027]
[0028] Note: A 1 It is a compound solution of 5% clove extract + 5% skullcap extract + 5% orange peel extract + 5% tea saponin plant extract.
[0029] A 2 It is a com...
Embodiment 3
[0035] Induced and resistive effects of biogenic pesticides with different volume ratios on camellia oleifera
[0036] After mixing the biogenic pesticides according to the volume ratio in Table 3, spray the liquid medicine on the 2-year-old Camellia oleifera seedlings until water drips from the leaves, and then spray the liquid medicine again after 2 days. The yellow-green young leaves that had been sprayed with the liquid medicine were collected and inoculated, and distilled water was used as a control. Each treatment had 15 replicates. Observation results. It can be seen from Table 3 that the mixed biogenic pesticides with different volume ratios can induce the resistance of camellia oleifera to the main diseases of camellia oleifera.
[0037] Lesion reduction rate = lesion area of control treatment - lesion area of induction treatment / lesion area of control treatment × 100%
[0038] Table 3 Induction and resistance effects of different proportions of biogenic pes...
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