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Pseudomonas strain and application thereof

A technology for pseudomonas and strains, applied in the field of pseudomonas strains, can solve problems such as poisoning, and achieve the effects of reducing production and use costs, low production and use costs, and broad degradation spectrum

Inactive Publication Date: 2011-01-05
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chromium (VI) is an important component of many chemical wastewaters. Chromium (VI) has obvious toxic effects on the human body; at the same time, the presence of chromium (VI) can significantly inhibit the biological removal of organic pollutants in wastewater. General biological It is difficult to make the effluent of the wastewater containing the above components meet the discharge standards

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1, the acquisition and identification of bacterial strain SG-1

[0028] 1. Obtaining of strain SG-1

[0029] In August 2008, 2 g of activated sludge from coking wastewater was taken from Shaoguan, Guangdong, and added to phenol-containing basal salt medium for 7 days of cultivation. After dilution, it was spread on a phenol-containing basal salt medium plate. After 7 days of cultivation, a single Colonies were preserved after streaking and purification.

[0030] 2. Identification of strain SG-1

[0031] Morphological characteristics: The bacteria are rod-shaped.

[0032] Physiological and biochemical characteristics: the main biological characteristics are G - , Gram-negative bacteria; positive for nitrate reduction and glucose fermentation acid production; negative for catalase reaction, gelatin hydrolysis, nitrite reduction reaction, methyl red reaction, oxidase, V.P. reaction, indole reaction; using Citric acid, cannot utilize tartaric acid and propion...

Embodiment 2

[0036] Embodiment 2, bacterial strain are to the degradation rate of different compounds in basal salt liquid medium

[0037] Basal salt liquid medium: mix 1.0g NaCl, 1.0g NH 4 NO 3 , 1.5g K 2 HPO 4 , 0.5g KH 2 PO 4 and 0.2g MgSO 4 ·7H 2 O was made up to 1L with water.

[0038]Pick a single colony of SG-1 and put it in 3ml LB liquid medium, shake it at 30°C and 165rpm for 24h to obtain fresh bacterial solution. Into 100ml of solution A (or solution B or solution C or solution D or solution E or solution F or solution G or solution H), add 5ml of fresh bacterial solution, and culture it on a shaker at 30°C (170 rpm), after 3 days (72 hours) Samples were taken to determine the degradation rate of the compound. The results are shown in Table 1.

[0039] The preparation method of solution A: add phenol and chromium (VI) into the basal salt liquid medium, the final concentration of phenol is 100 mg / L, and the final concentration of chromium (VI) is 2 mg / L.

[0040] The p...

Embodiment 3

[0049] Embodiment 3, the degradation rate of bacterial strain to phenol and the removal rate to chromium (VI) in tannery wastewater

[0050] Pick a single colony of SG-1 and put it in 3ml LB liquid medium, shake it at 30°C and 165 rpm for 24 hours to obtain fresh bacterial liquid. Measure 100ml of tannery wastewater, and detect the concentration (initial concentration) of chromium (VI) therein. The initial concentration of chromium (VI) is about 2mg / L. Add phenol to the tannery wastewater to make the final concentration 100mg / L; add 4ml of fresh bacterial solution, mix well, place it in a shaker at 30°C and 165 rpm, and take a sample to determine the compound after 3 days (72 hours) degradation rate. The results are shown in Table 2.

[0051] Table 2 Degradation rate of phenol and removal rate of chromium (VI) in tannery wastewater by strain SG-1 (3 days)

[0052] substrate

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PUM

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Abstract

The invention discloses a pseudomonas strain and application thereof. The strain provided by the invention is pseudomonas sp. SG-1 CGMCC No.3556. The microbial inoculum provided by the invention contains the active ingredient of pseudomonas sp. SG-1 CGMCC No.3556. The strain provided by the invention has a wider degradation spectrum and reaches the degradation rates to various toxic compounds over 75 percent. The microbial inoculum of the invention has the advantages of low production use cost, convenient use, wider degradation spectrum and good removal effect. The strain and the microbial inoculum provided by the invention are suitable for the biological treatment of Cr-contained wastewater generated in a tanning and dyeing process and wastewater generated in other petrochemical processes and can ensure that the toxic substance and Cr (VI) contents in the wastewater accord with the wastewater emission standard. The invention successfully solves the problem of poor toxic substance removal effect in a Cr-contained organic wastewater treatment process, reduces the workload in the production and use processes, decreases the production and use costs and has important meanings to protect the ecological environment and keep the body health of people.

Description

technical field [0001] The invention relates to a pseudomonas bacterial strain and application thereof. Background technique [0002] Aniline, phenol, cresol, p-chlorophenol, 1-naphthol, naphthalene and their derivatives are important chemical raw materials. Studies have found that these compounds are more harmful to humans and environmental organisms. Chromium (VI) is an important component of many chemical wastewaters. Chromium (VI) has obvious toxic effects on the human body; at the same time, the presence of chromium (VI) can significantly inhibit the biological removal of organic pollutants in wastewater. General biological It is difficult to make the effluent of the wastewater containing the above components meet the discharge standards. Microorganisms are often used as an important tool for environmental pollution remediation because of their powerful degradation functions and strong adaptability. Contents of the invention [0003] The purpose of the present inven...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A62D3/02C02F3/34C12R1/38A62D101/28A62D101/26A62D101/20A62D101/43C02F101/22C02F101/38C02F101/34C02F101/30C02F103/24
Inventor 吴晓磊孙纪全汤岳琴陈福明刘伟强赵晶晶郭鹏
Owner PEKING UNIV
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