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Injection-type cartilage bionic matrix for regenerative repair of cartilage and method for using same

A matrix material, injection-type technology, applied in the field of regenerative medicine, can solve the problems of inconvenience, difference, poor biocompatibility and safety, etc., and achieve the effect of improving safety, convenient use and good repair effect.

Inactive Publication Date: 2011-01-05
THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] To sum up, the construction of cartilage with a three-dimensional porous scaffold requires in vitro culture after inoculation of cells, and it is inconvenient to use. It must be shaped in advance to match the cartilage defect. Most of the biomaterials used in previous studies are artificially synthesized polymers. Material, which is significantly different from normal cartilage matrix components, and has poor biocompatibility and safety

Method used

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  • Injection-type cartilage bionic matrix for regenerative repair of cartilage and method for using same
  • Injection-type cartilage bionic matrix for regenerative repair of cartilage and method for using same
  • Injection-type cartilage bionic matrix for regenerative repair of cartilage and method for using same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The injection-type cartilage biomimetic matrix material used for cartilage regeneration and repair in this embodiment is prepared through the following steps:

[0030] a. Take 70% type II collagen, 28% chondroitin sulfate and 2% hyaluronic acid as raw materials by mass percentage;

[0031]b. Dissolve the raw material taken in step a with concentration of 0.05mol / L glacial acetic acid on a clean bench at 0°C to obtain a 10% acidic solution with a mass fraction of the raw material; of course, the raw material mass of the acidic solution in this step The score can be within the range of 10% to 30%;

[0032] c. Titrate and neutralize the acidic solution obtained in step b with an aqueous solution of sodium hydroxide with a concentration of 1 mol / L on a clean bench at 0°C until the pH is 7.0 to obtain the required injection-type cartilage for cartilage regeneration and repair Biomimetic matrix material; the sodium hydroxide used here can also be replaced by potassium hydrox...

Embodiment 2

[0036] The injection-type cartilage biomimetic matrix material used for cartilage regeneration and repair in this embodiment is prepared through the following steps:

[0037] a. Take 75% type II collagen, 24% chondroitin sulfate and 1% hyaluronic acid as raw materials by mass percentage;

[0038] b. Dissolving the raw material obtained in step a with hydrochloric acid with a concentration of 0.1mol / L on a clean bench at 4°C to obtain an acidic solution with a mass fraction of the raw material of 15%;

[0039] c. Titrate and neutralize the acidic solution obtained in step b with an aqueous solution of potassium hydroxide with a concentration of 1 mol / L on a clean bench at 4°C until the pH is 7.5 to obtain the required injection-type cartilage for cartilage regeneration and repair Biomimetic matrix materials.

[0040] The obtained injection-type cartilage biomimetic matrix material for cartilage regeneration and repair is sealed and stored at low temperature (0-4° C.) for futur...

Embodiment 3

[0042] The injection-type cartilage biomimetic matrix material used for cartilage regeneration and repair in this example was prepared through the following steps:

[0043] a. Take 65% type II collagen, 30% chondroitin sulfate and 5% hyaluronic acid as raw materials by mass percentage;

[0044] b. Dissolving the raw material obtained in step a with hydrochloric acid with a concentration of 0.01mol / L on a clean bench at 0°C to obtain an acidic solution with a mass fraction of the raw material of 20%;

[0045] c. Titrate and neutralize the acidic solution obtained in step b with an aqueous potassium hydroxide solution with a concentration of 2 mol / L on a clean bench at 4°C until the pH is 7.3 to obtain the required injection-type cartilage for cartilage regeneration and repair Biomimetic matrix materials.

[0046] The obtained injection-type cartilage biomimetic matrix material for cartilage regeneration and repair is sealed and stored at 0-4°C for future use.

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Abstract

The invention discloses an injection-type cartilage bionic matrix for the regenerative repair of cartilage and a method for using the same. The materials, of which the main components are the same as those of the extracellular matrix of the normal articular cartilage, are used as a raw material, and the raw materials undergo dissolution and pH adjustment to form the injection-type cartilage bionic matrix which is in a sol state at the low temperature and at a gel state at the body temperature; and by using the injection-type cartilage bionic matrix as a carrier, the injection-type cartilage bionic matrix is mixed with seed cells at the low temperature and then injected in cartilage deficiency parts, the injected materials undergo phase change by raising the temperature of the deficiency area to turn into the gel, so that the seed cells are fixed in the deficiency parts to conduct the regenerative repair function. The method is simple and easily implemented, the product is conveniently used, the operation injury is small, and the repair effect of the deficiency parts is good; the raw material has high biocompatibility and safety, all components undergo intermolecular bonding under the preparation conditions of the invention, so that the problem that the single bionic material is degraded too fast is solved; and the seed cells are directly mixed with the raw materials, and the culture in vitro is unnecessary so that the detention time in vitro of the seed cells are shortened, and the clinical application is safer.

Description

technical field [0001] The invention relates to the technical field of regenerative medicine, in particular to an injection-type cartilage biomimetic matrix material for cartilage regeneration and repair and a use method thereof. Background technique [0002] Cartilage defects in joints caused by trauma or bone disease are common clinically, and the existing treatment measures have obvious defects. The rapidly developing tissue engineering technology provides a new technology for its regeneration and repair. In 1984, the Swedish medical scientist Peterson et al first reported that in the rabbit articular cartilage defect model, the autologous periosteum was sutured to the periphery of the cartilage defect with absorbable sutures, and then the autologous chondrocyte suspension was injected into the defect, and the detection of articular cartilage at 16 weeks after operation confirmed that The defect is repaired by hyaline cartilage. In 1987, Brittberg of Gothenburg Medical C...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/26A61L27/24A61L27/20A61L27/38A61L27/52
Inventor 王富友杨柳张颖段小军戴刚宁志刚崔运利
Owner THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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