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Method for detecting single nucleotide polymorphism (SNP) of cattle MGAT2 gene

A single nucleotide polymorphism, gene technology, applied in the field of molecular genetics, can solve problems such as change

Inactive Publication Date: 2010-12-22
NORTHWEST A & F UNIV
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Problems solved by technology

The cSNP in the coding region of the gene can be divided into two types: one is the synonymous cSNP (Synonymous cSNP) in the coding region, that is, the change of the coding sequence caused by the SNP will not affect the change of the amino acid sequence in the translated protein The other is the non-synonymous cSNP (Non-Synonymous cSNP) in the coding region, that is, the change of the base sequence will lead to the change of the encoded amino acid, which will lead to the change of the amino acid sequence in the protein, which may eventually affect the function of the protein

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  • Method for detecting single nucleotide polymorphism (SNP) of cattle MGAT2 gene
  • Method for detecting single nucleotide polymorphism (SNP) of cattle MGAT2 gene
  • Method for detecting single nucleotide polymorphism (SNP) of cattle MGAT2 gene

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Embodiment Construction

[0028] a, the design of PCR primers containing the first exon region of cattle MGAT2 gene

[0029] Taking the bovine (NC_007313.4, Region: 54835024-54847263) sequence published by NCBI as a reference, Primer 5.0 was used to design PCR primers capable of amplifying the first exon region of the cattle MGAT2 gene. The primer sequences are as follows:

[0030] Upstream primer: cttgaaacgg caacccaccc act 23;

[0031] Downstream primer: agcccagcct gccttacgta aggc 24;

[0032] Using the above primers to amplify the cattle genome, it is possible to amplify a 237bp gene fragment comprising the 5'UTR of the cattle MGAT2 gene (NC_007313.4 sequence) and the 283bp to 519bp of the first exon region. The electrophoresis detection of the amplified fragment is as follows: figure 1 As shown, among them, lanes 1 to 6 are detection fragments, and lane M is Marker; after sequencing and identifying the amplified fragments, the sequence of 471bp to 519bp is as follows:

[0033] CCTGTACTGG ATCTTCTGC...

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Abstract

The invention discloses a method for detecting the single nucleotide polymorphism (SNP) of a cattle MGAT2 gene. The method comprises the following steps of: carrying out PCR (Polymerase Chain Reaction) amplification on the cattle MGAT2 gene by using the DNA containing the cattle MGAT2 gene of a cattle gene genome to be detected as a template and a primer pair p as a primer; digesting a PCR amplification product by using a restriction enzyme HaeIII and then carrying out agarose gel electrophoresis (AGE) on an amplified fragment after enzyme cutting; and identifying the SNP of the 495th site of the cattle MGAT2 gene according to an AGE result. Because the functions of the MGAT2 gene relate to the properties of weight, daily gain growth, and the like, the detection method lays a foundation for establishing a relation between the SNP of the MGAT2 gene and the growth properties so as to facilitate the mark assisted selection of the growth properties for cattle beef in China for quickly establishing cattle populations with good genetic resources.

Description

technical field [0001] The invention belongs to the field of molecular genetics, relates to the detection of gene single nucleotide polymorphism (SNP), in particular to a method for detecting the single nucleotide polymorphism of cattle MGAT2 gene. Background technique [0002] Gene polymorphism refers to the difference in genome sequence between different species or different individuals within the same species. These differences are caused by nucleotide changes in DNA alleles in chromosomes, mainly including base substitutions, insertions, deletions and duplications. Changes in sequence copy number. [0003] Single nucleotide polymorphism (Single Nucleotide Polymorphism, SNP) is a type of genetic marker system proposed by Lander (1996), a scholar at the Human Genome Research Center of the Massachusetts Institute of Technology. Polymorphism caused by acid (A / T / C / G) substitution. Its variant forms include: transversion, conversion, insertion and deletion, etc., which are m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 陈宏屈炼杨明娟刘俊霞蓝贤勇雷初朝
Owner NORTHWEST A & F UNIV
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