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Method for preparing electrostatic spinning immobilized laccase electrode

A technology of immobilizing laccase and electrospinning is applied in the field of electrochemical detection to achieve the effects of good thermal stability, low cost and excellent mechanical properties

Inactive Publication Date: 2011-09-28
BEIJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The method of in situ electrospinning to immobilize laccase to construct biosensors for the detection of pollutants has not been reported in the literature

Method used

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  • Method for preparing electrostatic spinning immobilized laccase electrode
  • Method for preparing electrostatic spinning immobilized laccase electrode
  • Method for preparing electrostatic spinning immobilized laccase electrode

Examples

Experimental program
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Effect test

Embodiment 1

[0031] 1) The surface of the glassy carbon electrode is rough ground and finely ground with emery paper, and then α-Al with a particle size of 0.05 μm 2 o 3 The powder is polished on the flat glass. After each polishing, the surface dirt is washed off first, and then moved into an ultrasonic water bath for cleaning, each time for 2 to 3 minutes, and repeated three times until it is cleaned. Finally, ethanol, V 硝酸 :V 水Wash thoroughly with 1:1 nitric acid and water to obtain a fresh electrode surface, and then place it in 0.2mol / L H 2 SO 4 Scanned by cyclic voltammetry until stable;

[0032] 2) Dissolve polyvinyl alcohol particles with a molecular weight of 100,000 and block copolymer F108 in ultrapure water, heat and stir until clarification after uniform mixing, wherein the mass fraction of polyvinyl alcohol is 6%, and the mass fraction of block copolymer F108 The mass fraction is 0.3%;

[0033] 3) Dissolve 1g of chloroauric acid in fresh ultrapure water at one time, and...

Embodiment 2

[0038] The 2,4-dichlorophenol solution in Example 1 was replaced with 80 μmol / L 4-chlorophenol solution, and other conditions were the same as in Example 1. Experiments showed that the laccase electrode had a good response to 4-chlorophenol.

Embodiment 3

[0040] The 2,4-dichlorophenol solution in Example 1 was replaced by 50 μmol / L 2-chlorophenol solution, and other conditions were the same as in Example 1. Experiments showed that the laccase electrode had a good response to 2-chlorophenol.

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Abstract

The invention belongs to the field of electrochemical detection of chlorophenol substances using an electrostatic spinning fiber immobilized enzyme electrode, and relates to preparation of a laccase electrode with response to chlorophenols and the detection of the chlorophenol substances by using the electrode. Particularly, environment-friendly macromolecular polymer polyvinyl alcohol is used asa raw material, and the laccase electrode is prepared by performing laccase immobilization by in-situ electrostatic spinning technology. The immobilized laccase electrode has response to 2,4-dichlorophenol, 4-chlorophenol, 2-dichlorophenol, 2,4,6-trichlorophenol and pentachlorophenol with low concentration; and the detection of the chlorophenol substances can be performed according to the position of reduction current peak of the response of the laccase electrode.

Description

technical field [0001] The invention relates to a preparation method of an enzyme electrode and the electrochemical detection field of using the enzyme electrode to detect pollutants in water. In particular, it relates to a method for making an enzyme electrode for detecting pollutants in water and applying the prepared enzyme electrode to the detection of low-concentration chlorophenols in water. Background technique [0002] The immobilized enzyme sensor integrates the specificity of enzyme reaction, the long-term stability of immobilized enzyme, and the characteristics of fast and sensitive electrochemical analysis, and is attracting people's attention. Many enzyme electrodes with theoretical significance and practical value are being used continuously. developed. Its application in the environment is mainly for the detection of phenolic substances. In the 1990s, scholars used glassy carbon electrodes modified by laccase and tyrosinase dual-enzyme systems for continuous...

Claims

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Application Information

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IPC IPC(8): G01N27/327D01F8/10D01F1/10D01D5/00
Inventor 牛军峰刘佳殷立峰江帆
Owner BEIJING NORMAL UNIVERSITY
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