New method for producing low polyxylose by utilizing microorganism

Abstract

The invention relates to a new method for producing low polyxylose by utilizing microorganism, which takes corncob as a raw material. The method comprises the following steps: smashing corncob, adding water, and carrying out acidification and high-temperature splitting decomposition to obtain mixed liquor of the raw material; directly inoculating fermented culture of aspergillus niger U8-7, carrying out microbial induction enzyme production zymolysing, and then separating, purifying, decoloring, desalinating, filtering, concentrating and spray drying to obtain the low polyxylose. The method of the invention leaves out the production link for producing low polyxylose, reduces production cost, and obviously increases the output capacity of low polyxylose.

Description

technical field [0001] The invention belongs to the application field of microbial enzyme engineering technology and is used for producing xylooligosaccharides from corncobs, in particular to a new method for producing xylooligosaccharides by using microorganisms. Background technique [0002] Xylooligosaccharides, also known as xylooligosaccharides, are oligosaccharides composed of 2-7 xylose molecules connected by β-1, 4 glycosidic bonds, mainly xylobiose and xylotriose. Xylo-oligosaccharides occur naturally in bamboo roots, fruits, vegetables, milk and honey, but in very small amounts. The main raw materials used to produce xylooligosaccharides are hardwood, corn cob, sawdust, bagasse, sugar beet bagasse, chaff, bran, etc. The sweetness of xylo-oligosaccharide is about 40% of that of sucrose, with pure sweet taste, dry product is light brown or light yellow powder, does not degrade at PH 0.8-1.5, has high acid stability, does not absorb moisture and returns to moisture, ...

AI Technical Summary

Problems solved by technology

In recent years, it has been reported to use Aspergillus niger to produce xylanase by solid fermentation, and then use xylanase to hydrolyze xylan to prepare xylooligosaccharides. However, this method needs to be cultivated at 28°C for 72 hours to obtain enzyme activity greater than 2500IU / g (medium dry weight) xylanase, not only takes a long time, but also increases the difficulty of the production process during the periodical pressure change operation in the production process, and the low enzyme activity cannot meet the needs of industrial production.

The method of using microbial fermentation culture to directly react with raw material liquid to prepare xylo-oligosaccharides has not been reported yet