Protein chip kit and method for detecting transgenic crops

Abstract

The invention discloses a protein chip for detecting transgenic products. The protein chip is a film which is arranged on the plane of a plane type supporting carrier and coated with a modified material; and protein antibody probes for detecting transgenic agricultural products are arranged on the surface of the film in a lattice mode and comprise 1) a Bt cry1Ac / Ab monoclonal antibody, 2) a Bt cry1Ah monoclonal antibody, 3) a phytase PHY monoclonal antibody, 4) a glyphosate-tolerant G2-EPSPS polyclonal antibody and 5) a salt-tolerant IrrE polyclonal antibody. The invention further provides a chip kit for detecting transgenic crops and performing result judgment simply and rapidly based on antigen antibody reaction, which belongs to the field of inspection and quarantine. The protein chip kit has the advantages of: 1) high speed, wherein the detection time is only 1.5 to 2 hours; 2) specificity, wherein protein antigens and antibodies are specifically combined and each antibody performs a point system process repeatedly for three times, so that the result correctness is greatly improved; 3) high flux, wherein the protein chip kit can detect the proteins of five kinds of commercial transgenic components at the same time; and 4) stability, wherein the results of the repeated tests show that the stability of the method is high.

Description

technical field [0001] The invention relates to a method for rapidly detecting transgenic crops based on transgenic components and a protein chip, in particular to a protein chip method and a kit for parallel detection of five commercial transgenic components in a reaction. The existing patent is to use ELISA test strip method for detection, and each component needs to be tested once, which is time-consuming and not conducive to rapid on-site detection. We select the commercialized protein antibody of the genetically modified component, immobilize it as a probe on the modified support, add the component to be tested to the chip matrix after pretreatment, and then add the labeled antibody, and use laser co- The focus scanner detects the results, and can directly determine whether there are components that need to be detected in the sample to be tested at one time. The result interpretation is intuitive, clear and fast, and the operation is simple and fast. It is suitable for s...

AI Technical Summary

Problems solved by technology

Because these methods have cumbersome operation steps and long detection time; they are not suitable for on-site real-time detection and tracking detection; the detection cost is too high; the knowledge, operation level and instrument level of the inspectors are too high, and the methods are difficult to popularize.

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