Method for constructing cryptococcus albidus engineering bacterial strain for producing oleic acid in high yield
A technology of Cryptococcus albicans and engineering strains, applied in the field of genetic engineering, can solve the problems of low oil production rate, large distance, complex composition of oil components, etc., and achieve the effect of increasing oil production rate and increasing the content of oleic acid
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[0019] Below in conjunction with accompanying drawing and embodiment the present invention will be further described:
[0020] 1 Experimental materials
[0021] 1.1 Plant material
[0022] The developing fruits of Tunga paulica were collected from the Arboretum of the Subtropical Forestry Research Institute of the Chinese Academy of Forestry, the seeds were peeled off, quick-frozen in liquid nitrogen, and stored in a -80°C refrigerator for later use.
[0023] 1.2 Strains and plasmids
[0024] Cryptococcus albidus was purchased from the Culture Collection Center of the Institute of Microbiology, Chinese Academy of Sciences, and Escherichia coli DH5α, Agrobacterium tumefaciens EHA 105, LBA4404, and AGL-1 were preserved in our laboratory. The plasmid pCAMBIA2300 was preserved in our laboratory, and the cloning vector pGEM-T Easy was purchased from Promega.
[0025] 1.3 Main biochemical reagents
[0026] RNA extraction kits were purchased from Tianze Gene Engineering Co., Ltd....
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