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Fusarium proliferatum, and bacterium agent and application thereof

A fusarium and microbial agent technology, applied in the field of agricultural microbes, can solve the problems of few varieties of rabies-resistant species, poor control effect, and environmental pollution, and achieve the effects of low cost, environmental friendliness, and simple preparation method

Inactive Publication Date: 2012-07-25
INST OF PLANT PROTECTION HEBEI ACAD OF AGRI & FORESTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the few varieties resistant to Lidang, and the problems of poor control effect and environmental pollution brought by chemical pesticide control, the object of the present invention is to provide a Fusarium argentia strain for biological control of Lidang, which can be highly efficient, Long-term inhibition of Helianthus japonica parasitizing on sunflower and tobacco, and is environmentally friendly

Method used

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  • Fusarium proliferatum, and bacterium agent and application thereof
  • Fusarium proliferatum, and bacterium agent and application thereof
  • Fusarium proliferatum, and bacterium agent and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment 1: the preparation of the liquid preparation of Br-1 microbial bacterial agent

[0046] (1) Strain activation: The low-temperature-preserved bacterial strain Br-1 (preserved in the General Microbiology Center of the China Microbiological Culture Collection Management Committee on April 23, 2010, and the preservation number is CGMCC No.3759) was placed on the PDA plate medium Activation (25°C) was carried out, and multiplied for later use;

[0047] (2) Preparation of seed liquid: make PDB liquid medium according to conventional methods, put 150mL PDB culture medium in 500mL Erlenmeyer flask (PDB medium: potato 200g, glucose 20.0g, distilled water 1000ml), autoclave and moist heat sterilization, wait After the temperature dropped to room temperature, insert the Br-1 strain activated in step (1) into each bottle, 5 pieces of bacterial slices with a diameter of 0.5 cm, and carry out shaking culture on a shaker at 25-26 ° C and a rotating speed of 170 rpm for 72 h...

Embodiment 2B

[0051] The preparation of the granule of embodiment 2Br-1 microbial bacterial agent

[0052] Steps (1) to (5) are the same as in Example 1, then the liquid preparation of the obtained Br-1 bacterial strain is concentrated about 10 times through sodium membrane filtration, and then concentrated according to the ratio of 1:7 between the liquid preparation and peat. Add peat to the liquid, mix and extrude to form granules of Br-1 strain, and the content of Br-1 live bacteria reaches 10 8 pcs / g.

Embodiment 3

[0053] Embodiment 3: Greenhouse Control Efficiency Test of Laminar Fusarium Br-1

[0054] The experiment was carried out in the greenhouse of the Institute of Plant Protection, Hebei Academy of Agriculture and Forestry Sciences. Tobacco seedlings were cultivated first, and when the tobacco seedlings grew 2-3 leaves, they were transplanted into flowerpots with a diameter of 16 cm, with one tobacco seedling per pot. Add 0.24g Liedang seeds to each pot of soil before tobacco transplanting, and mix well. According to the difference of the dilution concentration of the Br-1 liquid preparation prepared by the embodiment 1 applied, it is divided into 3 concentration treatments:

[0055] Process 1: Approx. 1.47×10 8 Conidia / ml;

[0056] Process 2: 1.47×10 7 Conidia / ml;

[0057] Process 3: 1.47×10 6 Conidia / ml;

[0058] CK: without adding Br-1 liquid preparation as a control;

[0059] Add 1ml of the above three concentrations of Br-1 liquid preparations to each pot, and repeat e...

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Abstract

The invention belongs to the field of agricultural microbiology and discloses a fusarium proliferatum strain Br-1 with the preserved number of CGMCC No. 3759. The invention also discloses a microbiological bacterium agent prepared by using the Br-1. The fusarium proliferatum strain Br-1 and the bacterium agent thereof have good inhibiting effect for orobanche cumana wallr, and the control effect in fields reaches 61.7-82.0%; and secondarily, a preparation method of the microbiological bacterium agent is simple, and has low cost and easy promotion and application. In addition, the invention belongs to a biological control method, has no pollution, no nuisance and low residue and is environment-friendly.

Description

technical field [0001] The invention belongs to the field of agricultural microorganisms, and in particular relates to a Fusarium laminarum, a microbial agent produced by using the Fusarium laminarum and its application. Background technique [0002] Orobanchaceae and Orobanche are annual root-parasitic weeds that parasitize on the roots of some higher plants without chlorophyll and root systems, and absorb nutrients and water from the host plant with suckers. In our country, the more harmful radangs are mainly sunflower radangs, branched radangs and Egyptian radangs. [0003] At present, the main methods of preventing and controlling Lie dang are farming, cultivation techniques or application of chemical agents; however, due to the large amount of Lie dang seeds produced, the seeds have a long survival period, and under suitable temperature and humidity, the seeds can germinate all year round, and the whole growth of the crops can be affected. During the period, there will...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/04A01N25/02A01N25/12A01P13/02C12R1/77
Inventor 孔令晓王连生栗秋生纪莉景
Owner INST OF PLANT PROTECTION HEBEI ACAD OF AGRI & FORESTRY SCI
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