Double-channel real-time bioaerosol monitoring method and device thereof

Abstract

The invention provides a double-channel real-time bioaerosol monitoring method and a device thereof. The method is characterized by comprising the following steps: (1) concentrating atmospheric aerosol particles; (2) carrying out double-channel ultraviolet induced fluorescence detection; (3) data-processing; (4) cleaning the surface of an enrichment board of the particles and regenerating; and (5) repeatedly carrying out a new round of loop detection. The device comprises an aerosol-particles concentration unit, a double-channel ultraviolet induced fluorescence detection unit, a particle enrichment board regeneration mechanism, a rotary table and a control system. The invention can measure the density and the change of bio-particles such as bacteria, spores, viruses and the like in an ambient atmosphere in real time so as to achieve an early warning function on bioaerosol content in the atmosphere.

Description

technical field [0001] The invention relates to atmospheric biological aerosol particles, in particular to a dual-channel real-time biological aerosol monitoring method and device, which can measure the concentration and changes of biological particles such as bacteria, spores, and viruses in the ambient atmosphere in real time, and realize the detection of biological gas in the atmosphere. Early warning function of sol content. Background technique [0002] Atmospheric aerosol particles refer to various solid particles and liquid microparticles suspended in the atmosphere, with a particle size between 10-3 μm and 102 μm. Among them, living biological substances or particles are collectively referred to as bioaerosol particles, including microorganisms such as viruses, bacteria, fungi, pollen, and spores. Bioaerosol particles can usually attach to non-biological particles in the atmosphere, such as microorganisms such as bacteria; some can also be directly suspended in the ...

AI Technical Summary

Problems solved by technology

[0008] 1. The detection sensitivity is low, the cycle is long, and it does not have real-time performance

Traditional detection methods need to go through steps such as collection and cultivation, and the cultivation process generally needs to be carried out in a laboratory environment with strict requirements, and cannot be operated in a field environment

[0009] 2. The detection equipment is large in size and requires high conditions of use, which is not convenient for on-site and mesh point monitoring

The ultraviolet light source used in this method is generally a xenon lamp or an ultraviolet laser obtained by frequency doubling of a near-infrared solid-state laser (such as a Nd:YAG laser). This type of ultraviolet light source has high power consumption, large structure size, and complex optical path structure.

[0010] 3. The structure is relatively complex and requires high stability of the gas path, which is not applicable when the concentration of atmospheric aerosol is too high

The single particle ultraviolet light-induced fluorescence detection method requires the design of a dedicated single aerosol particle generation gas path, and the flow rate is low. When the aerosol concentration is too high, there will be a large overlap error

[0011] 4. The false negative rate is high

Due to the limitations of light source conditions and equipment volume, single-particle ultraviolet light-induced fluorescence detection generally uses a single-wavelength ultraviolet light source (266nm, 280nm, 355nm or 365nm), which has low fluorescence excitation efficiency for some biological particles and insufficient detection sensitivity. prone to underreporting

[0012] 5. High false alarm rate

The single particle ultraviolet light-induced fluorescence detection method uses a single wavelength ultraviolet light source, it is difficult to obtain enough absorption spectrum and fluorescence spectrum information of the detected particle to distinguish, and it is easy to cause misjudgment

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