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Method for preparing probiotic microcapsules by using electrostatic spraying

An electrostatic spray and probiotic technology, which is applied in the field of electrostatic spray preparation of probiotic microcapsules, can solve the problems of limitation and low encapsulation rate by emulsification method, and achieve the effects of good encapsulation performance, low price and easy-to-obtain wall materials.

Inactive Publication Date: 2010-10-13
UNIV OF SHANGHAI FOR SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the microencapsulation methods reported in the literature include emulsification method and extrusion method. The encapsulation efficiency of the emulsification method is low, only about 60%. Food applications are limited

Method used

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  • Method for preparing probiotic microcapsules by using electrostatic spraying
  • Method for preparing probiotic microcapsules by using electrostatic spraying
  • Method for preparing probiotic microcapsules by using electrostatic spraying

Examples

Experimental program
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Effect test

Embodiment 1

[0029] Mix the sodium alginate solution with a concentration of 1.5% and the gelatin solution with a concentration of 1%. , a lactic acid bacteria suspension with a viable count of about 1.1×109cfu / ml (the colony form of lactic acid bacteria is as follows figure 1 shown) and the above-mentioned wall material in a mass ratio of 1:2 and sucked into the syringe, then use the microcapsule molding device of the high-voltage electrostatic field, select the electric field strength as 3.7 × 10kV, and the distance between the syringe needle and the calcium chloride liquid surface The distance is 2.5 cm and the advancing speed of the syringe is 50 mm / h. Spray the mixture into a sterilized 3% calcium chloride solution with constant stirring, Ca 2+ Cross-linked with sodium alginate to form microcapsules, and left to solidify in calcium chloride solution for 20 minutes. Filter and wash with physiological saline for 2-3 times to prepare lactobacillus microcapsules.

[0030] The same wall...

Embodiment 2

[0034] Will grow to the logarithmic phase Bacillus subtilis bacterium liquid (number of live bacteria is 1.23 * 109cfu / ml, Bacillus subtilis bacterium colony form is as follows image 3shown), centrifuged at 4° C. for 20 min in a J-20XP high-speed refrigerated centrifuge (7000 rpm), removed the supernatant, and collected the sludge. Add the same volume of physiological saline as the culture medium to the bacteria slime, mix well and set aside; mix 1.5% sodium alginate solution and 1% gelatin solution, filter and sterilize with a microporous filter membrane (pore size 0.22 μm) as a wall material Then, inhale the syringe after mixing the Bacillus subtilis suspension with the wall material; Utilize the microcapsule forming device (ZL00218100.2) of the high-voltage electrostatic field, select the electric field strength to be 4~6kV, the syringe needle and the calcium chloride liquid level The distance between them is 20-30mm, the advancing speed of the syringe is 50-70mm / h, and th...

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Abstract

The invention discloses a method for preparing probiotic microcapsules by using electrostatic spraying. Sodium alga acid and gelatin are taken as wall materials, probiotic suspension or microporous starch-adsorbed probiotics are taken as core materials, and calcium chloride is taken as solidifying solution. The method comprises the following steps of: a) mixing 1.5 percent solution of sodium alga acid and 1 percent gelatin in the same volume, and uniformly mixing with the probiotic suspension or microporous starch-adsorbed probiotics to form mixed solution; b) putting the mixed solution into a container, and injecting the mixed solution into 2 to 3 percent continuously stirred solution of calcium sodium chloride in the electrostatic spraying mode by using a microcapsule molding device to form microcapsules; c) standing and solidifying the encapsulated probiotic microcapsules for 20 minutes in the solution of calcium chloride, and filtering and cleaning the solution by using physiological saline; and d) saving the probiotic microcapsules in the physiological saline. The prepared microcapsules have the advantages of uniform grain diameter, good encapsulation performance, simple process, readily available wall materials, and low cost, and can be widely applied to the fields of food processing, biomedicines, chemical industry, and the like.

Description

technical field [0001] The invention relates to a method for preparing probiotic microcapsules by electrostatic spraying, which maintains the activity of probiotics and is applied in technical fields such as health food and biological feed. Background technique [0002] Currently recognized probiotics include Lactobacillus, Bifidobacterium, Streptococcus, Bacillus, and yeast. A large number of research reports and clinical trials have confirmed that probiotics such as: Lactobacillus acidophilus, as a normal flora in the human intestinal tract, can relieve lactose intolerance, inhibit tumor cell formation, lower cholesterol levels, relieve diarrhea and enhance the body's immunity, etc. Physiological function is an important microorganism in the human intestinal tract, which is closely related to the health of the human body. It is a probiotic that attaches great importance to research and development in the lactic acid bacteria family. It is regarded as the third-generation y...

Claims

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Application Information

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IPC IPC(8): B01J13/04A23L1/29A23P1/04A23L33/00A23P10/30
Inventor 李保国冯琼应月
Owner UNIV OF SHANGHAI FOR SCI & TECH
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