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Coprinus atramentarius and cultivation method thereof

A technology of Coprinus japonica and cultivars, applied in botany equipment and methods, fertilizer mixtures, horticulture, etc., can solve the problem of unsuitable scale production of Coprinus japonica species, high requirements for nutrients and environmental conditions, and no separation problems such as strains, to achieve high-yield, high-quality, large-scale production, improve environmental adaptability, and increase the number of effects

Active Publication Date: 2013-01-23
BEIJING UNIV OF AGRI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the research on Coprinus mori is still in the laboratory stage, and strains suitable for large-scale production have not been isolated. This may be due to the high requirements for nutrients and environmental conditions of Coprinus mori.
In addition, in the laboratory stage, the composition of the culture medium used for the mother species, original species or fruiting bodies of the existing Coprinus japonica species is complicated, and some components are relatively expensive, and are not suitable for the scale of the Coprinus japonica species Production

Method used

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  • Coprinus atramentarius and cultivation method thereof
  • Coprinus atramentarius and cultivation method thereof
  • Coprinus atramentarius and cultivation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0074] The separation and cultivation of embodiment 1 Chinese ink Coprinus sp.

[0075] In May 2008, the fruiting body of the fresh ink-colored Coprinus was collected from the decaying willow in Huairou, Beijing. Use 75% alcohol cotton ball to wipe off the soil on the surface, divide the fruiting body into two by hand, take a tissue piece the size of a grain of rice at the junction of the cap and the stipe under sterile conditions, and put it into a test tube of PDA comprehensive medium . After the mycelia germinated, they were transferred to the same blank test tube and preserved as test tube strains.

[0076] During the cultivation process, it was found that the PDA comprehensive medium was used to cultivate the strains, and the mycelium grew rapidly, and it took 6 days to cover the petri dish, but the mycelia were sparse and the mycelium density was low. After transferring the tube twice continuously, the hyphae became stronger and the time to fill the tube became longer. ...

Embodiment 2

[0077] The determination of embodiment 2 carbon source

[0078] The present inventor tests the carbon source of the female culture medium used for cultivating the female species in order to find a more suitable female culture medium for the cultivation of the female species of Coprinus chinensis of the present invention.

[0079] The formula of the culture medium used in this experiment is peptone 5g, KH 2 PO 4 1g, MgSO 4 0.5g, vitamin B 1 0.01% by weight, 20 g of agar, 1000 mL of water, and natural pH. Then prepare 8 parts of carbon source determination medium, each 250ml, 1 of which does not add any material as a control, and adds 2% of sucrose, 2% of glucose, 2% of cornstarch, 2% of Glucose+2% corn starch, 2% lactose, 2% maltose and 2% fructose, a total of 7 treatments. 10 petri dishes (diameter 9cm) are subpackaged after each processing sterilization, and the test tube bacterial classification is activated and inoculated in the PDA medium (the content of potato is 20%...

Embodiment 3

[0084] The determination of embodiment 3pH value

[0085] Use 1mol / L NaOH or 1mol / L HC1 to adjust the pH of the basal medium with glucose + cornstarch as the carbon source to the gradient shown in Table 2 according to the needs, pack them in Erlenmeyer flasks, sterilize, and use ultra-clean workbench Invert the plate, 6 replicates for each treatment, inoculate the Coprinus japonica, and then culture at a constant temperature of 25°C. After 4 days, measure the diameter of the colony every other day as an indicator of mycelium growth; record the number of days when the plate is full (i.e. the number of days required to cover the petri dish) and observe the growth of the mycelia, and calculate the daily average growth rate of the mycelia.

[0086] After observation and records, it was found that the mycelia of Coprinus japonica began to germinate 3 to 4 days after inoculation, and the mycelium grew faster on the 6th to 10th day, and the mycelium growth rate was very different und...

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Abstract

The invention provides coprinus atramentarius and a cultivation method thereof, and also provides a culture medium for culturing parent species, original species or cultivated species of the coprinus atramentarius and the culture medium used in bag culturing. The preservation number of the coprinus atramentarius is CGMCC No.3578; the culture medium has the advantages of simple formula, easy preparation and the like; and the cultivation method can improve the environment adaptability of the coprinus atramentarius strains, the reproductive efficiency of the parent species and the nutrition absorption efficiency of the original species and the cultivated species, improve the commodity value of the coprinus atramentarius and realize mass production of the coprinus atramentarius.

Description

technical field [0001] The present invention relates to wild edible medicinal fungus and the cultivation technology of the fungus, in particular, the present invention relates to a kind of Coprinus indica and the cultivation method of the Coprinus indica. Background technique [0002] Coprinus atramentarius (Bull.) Fr., also known as ghost cap, ghost umbrella, haunted house, ghost fungus or towards life cover, belongs to the fungus Basidiomycetes Agaricaceae Agaricaceae Coprinus genus. The fruiting body of the Coprinus mori can be used as medicine. Chinese medicine believes that the fungus is cold in nature and sweet in taste, which is beneficial to the stomach, regulating qi and reducing phlegm, detoxifying and reducing swelling. Sore gangrene [Yang Shangguang. Medicinal fungi Xi'an: Shaanxi People's Publishing House. 1986.54-55]. [0003] Ink ghost umbrellas are distributed in Hebei, Shanxi, Jiangsu, Taiwan, Sichuan, Qinghai and Gansu, etc., and generally appear in orchar...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G1/04C05G3/00
Inventor 陈青君谢书清郝册程继鸿范双喜韩莹琰
Owner BEIJING UNIV OF AGRI
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