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Method for screening recombinant vaccinia virus containing exogenous fusion gene HIV/env/IFN alpha-2b

A vaccinia virus, fusion gene technology, used in microorganism-based methods, viruses/phages, biochemical equipment and methods, etc.

Inactive Publication Date: 2010-09-29
邢安辉 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no effective drug that can fundamentally treat and prevent AIDS

Method used

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  • Method for screening recombinant vaccinia virus containing exogenous fusion gene HIV/env/IFN alpha-2b
  • Method for screening recombinant vaccinia virus containing exogenous fusion gene HIV/env/IFN alpha-2b

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1: Construction diagram of recombinant expression plasmid pJ16env / IFNα-2b (see accompanying drawing 1)

Embodiment 2

[0017] Example 2: Recombinant plasmid digestion and identification diagram (see Figure 2).

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Abstract

The invention belongs to the biotechnology field of genetic engineering vaccine, providing a method for screening recombinant vaccinia virus containing exogenous fusion gene HIV / env / IFN alpha-2b. According to the action principle that the epitope of HIV outer membrane protein has neutralizing antibody determinant which affects virus to attack CD4+ tropism after combined with a target cell; recombinant expression plasmid pJ16env / IFN alpha-2b is constructed by using the method; adopting the liposome dye-transfer process and taking wild vaccinia virus as a transformation gene cause the pJ16env / IFN alpha-2b and the wild vaccinia virus to generate homologous recombination; and the HA characteristic is utilized to screen and purify the pJ16env / IFN alpha-2b recombinant vaccinia virus strain so as to lead the env / IFN alpha-2b fusion gene and the vaccinia virus to be co-expressed. A mice experience indicates that co-expression product of the env / IFN alpha-2b fusion gene and the vaccinia virus can improve the humoral immunity and the cellular immunity of a laboratory mouse; and the expressed protein product can be served as the antigen for immunoprophylaxis. The invention provides an important path for obtaining the outer membrane protein of HIV for researching the antigenicity of HIV structural protein.

Description

Technical field: [0001] The invention belongs to the technical field of genetic engineering vaccines, and relates to a method for screening recombinant vaccinia viruses containing exogenous fusion gene HIV / env / IFNα-2b. Background technique: [0002] AIDS (Acquired Immunodeficiency Syndrome, AIDS) is the most serious infectious disease threatening human beings since this century. The irreversible infections and tumors caused by the destruction of human immune function by HIV have threatened the survival and development of human society. In particular, one of the most striking phenomena during the spread of AIDS is that adolescents account for more than half of the new HIV infections worldwide. At present, there is no effective drug that can fundamentally treat and prevent AIDS. Existing various therapeutic measures are all tentative. Since AIDS is mainly a disease related to the immune system, obtaining reliable immune protection is the key to fundamentally solving the epi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N21/33C12N7/01C12R1/93
Inventor 邢安辉王洪军于宁
Owner 邢安辉
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