Method for preparing rabies virus antigen by chloroplast

Abstract

The invention provides a method for preparing a rabies virus antigen by chloroplast, comprising the following steps: (1) constructing a chlamydomonas chloroplast expression vector of rabies virus antigen gene; (2) screening the constructed expression vector transgenosis chlamydomonas to obtain transgenosis chlamydomonas; and (3) culturing transgenosis chlamydomonas to express the rabies virus antigen gene in chlamydomonas to obtain the rabies virus antigen. In the method of the invention, a chlamydomonas chloroplast expression system is utilized to produce rabies virus antigens safely and efficiently in a chlamydomonas bioreactor, the expressed protein has high specificity and good immunity effect, and can not produce non-purposive immunoreaction. The cost of the method of the invention is significantly lower than that of a traditional method for preparing the rabies virus antigen, the method has no need of investing and building factories and no generation of three wastes (waste gas, waste water and industrial residue), and the consumption of electric power, water resource and other resources is extremely low. The antigen prepared by the method of the invention has very high security and no need of purification, and can be directly used as animal supplementary feed, thus having treatment effect.

Description

technical field The invention relates to a method for preparing an antigen, in particular to a method for preparing a rabies virus antigen by using Chlamydomonas chloroplasts, and belongs to the field of preparation of rabies virus antigens. Background technique Rabies is a zoonotic and fatal encephalomyelitis caused by Rabies Virus (RV) invading the central nervous system. Once it develops, the mortality rate is almost 100%, posing a serious threat to human life safety. The disease is distributed globally, especially in Asia, especially in my country, where it is more prevalent and poses a serious threat to human life. Vaccine immunization is still the most reliable means to prevent and control rabies, so large-scale preventive immunization to dogs, cats and wild animals is the fundamental measure to control and eliminate rabies. At present, the rabies vaccines for veterinary use in my country mainly include inactivated vaccines and attenuated vaccines. Although tradition...

AI Technical Summary

Problems solved by technology

However, these bioreactors have their own deficiencies. For example, the use of proteins expressed by E. coli for immunization may inhibit beneficial normal E. coli due to the presence of E. coli in animals. In addition, proteins expressed by silkworms are used for immunization. Due to the presence of some highly homologous proteins between the silkworm and the immunized animals, some unintended immune responses may occur

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