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Sansevieria trifasciata saponin, preparation method and application thereof

A technology of blue saponin and tiger Pilan, which is applied in the field of tiger Pilan saponin and its preparation, can solve the problems of remarkable curative effect and achieve the effects of improving leaching efficiency, low cost and simple operation

Inactive Publication Date: 2010-08-18
JILIN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no single-component steroidal saponins directly used in clinical medicine, but total steroidal saponins are widely used in clinical practice as drugs for the treatment of cardiovascular and cerebrovascular diseases, and the curative effect is remarkable.

Method used

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  • Sansevieria trifasciata saponin, preparation method and application thereof
  • Sansevieria trifasciata saponin, preparation method and application thereof
  • Sansevieria trifasciata saponin, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] 1) Take 10 g of dry tiger Piran sample powder, soak it for 24 hours with 70% ethanol as extractant,

[0033] 2) Use a KQ-3200E ultrasonic generator (Kunshan Ultrasonic Instrument Co., Ltd.), adjust the temperature to 40°C, and carry out ultrasonic extraction for 50 minutes under the condition that the solid-liquid ratio is 1:15;

[0034] 3) Evaporate the extractant with a RE-52A rotary evaporator (Shanghai Yarong Biochemical Instrument Factory) until there is no alcohol smell, extract the remaining solution with petroleum ether for 3 times, and discard the ether layer.

[0035] 4) Extract with saturated n-butanol for 3 times, and then evaporate the n-butanol layer to dryness with a rotary evaporator to obtain the crude extract of total saponins.

Embodiment 2

[0037] 1), take the dry tiger Piran powder, after soaking overnight with 90% ethanol;

[0038] 2) In the HH-S6 digital display constant temperature water bath produced by Changzhou Huaputa Teaching Instrument Co., Ltd., heat to 75°C, the solid-liquid ratio is 1:12, and reflux extraction 4 times, each 4h;

[0039] 3) The extracts were combined, filtered, evaporated and concentrated with a RE-52A rotary evaporator (Shanghai Yarong Biochemical Instrument Factory) until alcohol-free.

[0040] 4), the concentrated extract was extracted 3 times with petroleum ether, and the ether layer was discarded;

[0041] 5), then extracted with saturated n-butanol for 3 times, recovered the n-butanol layer and concentrated to dryness with a rotary evaporator to obtain the total saponins extract of Hupilan.

Embodiment 3

[0043] 1) Take the dried tiger Pilan powder, add water at a ratio of 1:2, freeze and thaw repeatedly 3 times, and dry;

[0044] 2) soak overnight with 90% ethanol;

[0045] 3) In the HH-S6 digital display constant temperature water bath produced by Changzhou Huapuda Teaching Instrument Co., Ltd., heat to 75°C, the solid-liquid ratio is 1:12, and reflux extraction 4 times, each time 4h;

[0046] 4) Combine the extracts, filter, evaporate and concentrate with RE-52A rotary evaporator (Shanghai Yarong Biochemical Instrument Factory) until there is no alcohol smell.

[0047] 5) The concentrated extract was extracted 3 times with petroleum ether, and the ether layer was discarded;

[0048] 6) Extract with saturated n-butanol for 3 times, recover the n-butanol layer and concentrate to dryness with a rotary evaporator to obtain the total saponins extract of Hupilan.

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Abstract

The invention discloses sansevieria trifasciata saponin, a preparation method and application thereof. The method comprises the following steps of: making the sansevieria trifasciata into dry powder, extracting by using water, alcohol or methanol, extracting by using petroleum ether or ether, and extracting by using butyl alcohol or pentanol to obtain the sansevieria trifasciata total saponin extract. The method for preparing the sansevieria trifasciata saponi optimizes the process conditions, has simple operation and low cost, has low requirement on process equipment, can reduce the consumption of solvents and enhance the leaching rate. The sterilized sansevieria trifasciata has obvious inhibition function on the growth of JurKet, has no activity killing effect on human cervical carcinoma cells (HeLa) and human gastric carcinoma cells (BGC823), and has no injury to normal Chinese Hamster Ovary (CHO), and the cell grows normally without any obvious change.

Description

technical field [0001] The present invention relates to the extraction of a kind of plant active ingredient, specifically saponin and its preparation method and application Background technique [0002] Sansevieria trifasciata, also known as Sansevieria trifasciata, is a perennial succulent herb of the Agaveaceae and Sansevieria genus. Native to Africa and India, it belongs to desert plants, can endure harsh environmental conditions, has strong adaptability to the environment, is easy to reproduce, grows fast, likes warm climates, and is not cold-resistant. The suitable temperature for growth is 20-30°C. It will stop growing when it is lower than 13°C, and the temperature for overwintering cannot be lower than 8°C. Tiger Pilan has a creeping rhizome, the leaves grow directly from the rhizome, and the leaves are firm and upright. [0003] At present, many reports point out that tiger Pilan has the effect of purifying indoor air. It can release oxygen while absorbing carbon...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/88A61P35/02
Inventor 李泽鸿张璐张国利张继元李昌浩刘文涛刘洪章康立娟李振华樊敏
Owner JILIN AGRICULTURAL UNIV
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