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Novel cultural method of antrodia camphorata mycelium

A cultivation method and mycelium technology, which is applied in the novel cultivation field of Antrodia camphorata mycelium, can solve the problems such as difficulty and influence on yield, and achieve the effects of eutrophication, shortening days of tank storage, and promoting the growth of mycelium

Active Publication Date: 2010-08-18
GRAPE KING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The second difficulty is to find a hollow tree trunk from a large forest, which is not easy
[0017]4. The fermentation and cultivation of Antrodia camphorata mycelium is accompanied by a large amount of foam, which seriously affects its yield

Method used

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  • Novel cultural method of antrodia camphorata mycelium
  • Novel cultural method of antrodia camphorata mycelium
  • Novel cultural method of antrodia camphorata mycelium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] The cultivation of embodiment 1 Antrodia camphorata mycelium

[0048] Mycelium strains:

[0049] They are strains CGMCC NO.0575 and CGMCC NO.0543 deposited in the General Microbiology Center of China Microbiological Culture Collection Management Committee.

[0050] Plate culture:

[0051] The mycelium was inoculated on a plate, and cultured at 30° C. for about 2 weeks using potato dextrin medium (Potato Dextrose Agar, PDA).

[0052] Flask culture:

[0053] Scrape the hyphae on the plate and inoculate in the flask, use the following medium, at about 30°C, pH 4.5, shake and culture on a shaker at a shaking rate of 50-250rpm until the early log phase, that is, about 5-7 days ;

[0054] Medium formula

[0055] Element Concentration (add ingredients in 100ml water

[0056] mass in grams)

[0057] Cereals (such as wheat flour)

[0058] 1

[0059] Peptone 0.1

[006...

Embodiment 2

[0073] The dry weight of embodiment 2 Antrodia camphorata mycelium, polysaccharide body, Lab value and sesamol content determination

[0074] After freeze-drying or spray-drying the fermented liquid of Antrodia camphorata obtained by each group in Example 1, the dry weight, polysaccharide body, Lab value and sesamol content were determined.

[0075] Polysaccharide determination:

[0076] Polysaccharide analysis was carried out by the phenol-sulfuric acid method, please refer to the literature of Dubio et al. (Dubio, M. et al., 1956. Colorimetric method for determination of sugars and related substances. Anal. Chem. 28, 350.).

[0077] 1. Sample handling:

[0078] Take 1 gram of homogeneous powder, add 99 times the volume of distilled water, heat and extract at 121°C for 30 minutes, filter the supernatant with No. 1 filter paper into a concentration bottle, repeat the extraction step twice, collect the filtrate for concentration, and constant volume to 10ml. Then dialyze w...

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Abstract

The invention discloses a novel cultural method of antrodia camphorata mycelium. In the culturing process of the cultural method, sesame oil is added into a fermentation tank culture medium which is used for replacing the existing chemical defoaming agent. The invention provides a novel natural defoaming agent, and fermentation liquid of the antrodia camphorata mycelium obtained by the cultural method leads the tank collecting days of the antrodia camphorata mycelium to be shortened and the dry weight thereof to be increased, and ensures the color of the antrodia camphorata mycelium to be similar to red of the natural antrodia camphorata; and the antrodia camphorata mycelium is rich in sesamol and has higher nutritional value compared with the antrodia camphorata mycelium cultured by the existing method.

Description

technical field [0001] The invention relates to a novel cultivation method of Antrodia camphorata (Antrodia camphorata or Antrodia cinnamomea) mycelium. Background technique [0002] Types of Antrodia camphorata [0003] Antrodia camphorata is also known as cinnamomum camphora, camphor mushroom, and camphor mushroom. In Taiwan, it is called yin and yang counterpart mushroom. The fruiting bodies of Antrodia camphorata are perennial, with a strong camphor tree aroma, which is quite different from the general Ganoderma lucidum, and its appearance is plate-shaped or bell-shaped. Plate-shaped person, the surface is orange-red (yellow) color, and there are bacteria holes all over the surface. The bottom layer of the plate has light yellow-white cork, which grows on the inner wall of the hollow wood of the camphor tree through the cork. For bell-shaped ones, the hymenium (clock face) is also orange (yellow), full of fungal pores (4-5 bacterial pores / mm), and there are spores in i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04C05G1/00C12N1/14
Inventor 陈劲出许胜杰林定威
Owner GRAPE KING
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