Novel cultural method of antrodia camphorata mycelium
A cultivation method and mycelium technology, which is applied in the novel cultivation field of Antrodia camphorata mycelium, can solve the problems such as difficulty and influence on yield, and achieve the effects of eutrophication, shortening days of tank storage, and promoting the growth of mycelium
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Embodiment 1
[0047] The cultivation of embodiment 1 Antrodia camphorata mycelium
[0048] Mycelium strains:
[0049] They are strains CGMCC NO.0575 and CGMCC NO.0543 deposited in the General Microbiology Center of China Microbiological Culture Collection Management Committee.
[0050] Plate culture:
[0051] The mycelium was inoculated on a plate, and cultured at 30° C. for about 2 weeks using potato dextrin medium (Potato Dextrose Agar, PDA).
[0052] Flask culture:
[0053] Scrape the hyphae on the plate and inoculate in the flask, use the following medium, at about 30°C, pH 4.5, shake and culture on a shaker at a shaking rate of 50-250rpm until the early log phase, that is, about 5-7 days ;
[0054] Medium formula
[0055] Element Concentration (add ingredients in 100ml water
[0056] mass in grams)
[0057] Cereals (such as wheat flour)
[0058] 1
[0059] Peptone 0.1
[006...
Embodiment 2
[0073] The dry weight of embodiment 2 Antrodia camphorata mycelium, polysaccharide body, Lab value and sesamol content determination
[0074] After freeze-drying or spray-drying the fermented liquid of Antrodia camphorata obtained by each group in Example 1, the dry weight, polysaccharide body, Lab value and sesamol content were determined.
[0075] Polysaccharide determination:
[0076] Polysaccharide analysis was carried out by the phenol-sulfuric acid method, please refer to the literature of Dubio et al. (Dubio, M. et al., 1956. Colorimetric method for determination of sugars and related substances. Anal. Chem. 28, 350.).
[0077] 1. Sample handling:
[0078] Take 1 gram of homogeneous powder, add 99 times the volume of distilled water, heat and extract at 121°C for 30 minutes, filter the supernatant with No. 1 filter paper into a concentration bottle, repeat the extraction step twice, collect the filtrate for concentration, and constant volume to 10ml. Then dialyze w...
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