Molecular marker identification method of black fungus strain and specific molecular marker primers of black fungus strain HW 5

Abstract

The invention relates to a molecular marker identification method of a black fungus strain and specific molecular marker primers of black fungus strain HW 5, which solves the problems of high screening quantity of specific primers, difficult acquirement of specific markers, unavailable identification of filial generation and unavailable determination of genetic stability existing in the traditional molecular marker identification method of the black fungus strain. The identification method comprises the following steps of: (1) extracting the total DNA of mycelium; (2) carrying out ISSR-PCR (Inter-Simple Sequence Repeat and Polymerase Chain Reaction) analysis; (3) purifying and designing the specific molecular marker primers; and (4) carrying out PCR detection. The specific molecular marker primers of the black fungus strain HW 5 are R1-1 and R1-2. The molecular marker identification method of the black fungus strain has the advantages of small screening quantity of specific primers and easy acquirement of specific markers, and moreover, specific molecular marker genes obtained in the molecular marker identification method of the black fungus strain are completely and regularly distributed in a mononuclear filial generation.

Description

technical field [0001] The invention relates to a method for identifying black fungus strains and specific molecular marker primers for black fungus strain HW5. Background technique [0002] The identification of strain markers is a very important link in the cross-breeding of edible fungi. The traditional morphological markers are generally identified from the shape, character and physiological characteristics, but the morphological characteristics of fungi that can be used for classification and identification are relatively few and easy to identify. Affected by the external environment or human factors, the accuracy of morphological markers in the identification of fungal germplasm resources is low. Biochemical markers are mainly isozyme markers, but each isozyme marker requires a special color development method and technology. Isozyme is the product of gene expression rather than the gene itself, and is often affected by the environment and the developmental state of ed...

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Problems solved by technology

[0004] The purpose of the present invention is to solve the current molecular marker Auricularia auricula strain identification method with a large amount of specific primer screening, specific markers are difficult to obtain, and cannot identify hybrid offspring and determine its genetic Stability issues, and provide a black fungus strain molecular marker identification method and the specific molecular marker primers of black fungus strain HW5

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