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Ethionine resistance Candida utilis and application thereof

A technology of Candida utilis and ethionine, which is applied in fermentation, biochemical equipment and methods, microorganisms, etc., to achieve the effects of broad growth nutrition spectrum, low ethanol production, and good industrial application prospects

Inactive Publication Date: 2010-07-21
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] At present, research at home and abroad is mainly focused on the fermentation of single-purpose products such as SAM or GSH. So far, no Candida utilis has been selected for the co-production fermentation of SAM and GSH.

Method used

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  • Ethionine resistance Candida utilis and application thereof
  • Ethionine resistance Candida utilis and application thereof
  • Ethionine resistance Candida utilis and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1, co-production fermentation of SAM and GSH, and determining the optimal concentration of ethionine

[0030] (1) strain

[0031] The starting strain was Candida utilis (Candida utilis) SZU 07-01, which was preserved by the Industrial Microbiology Laboratory of Soochow University.

[0032] (2) culture medium

[0033] Slope and seed medium (g / L): glucose 20, peptone 20, yeast extract 10, pH 6.0;

[0034] Screening medium: add 0-0.7g / L ethionine to the seed medium;

[0035] Fermentation medium before optimization (g / L): glucose 30, ammonium sulfate 8, potassium dihydrogen phosphate 3, magnesium sulfate 0.25, pH 5.5.

[0036] (3) UV mutagenesis

[0037] Irradiation was carried out under Philips ZWSSJD-30 ultraviolet lamp, the irradiation power and wavelength were 30W, 253.7nm, respectively, and the irradiation distance was 30cm. The specific operation steps are: absorb 1 mL of seed culture solution after 20 hours of cultivation, centrifuge at 10,000 r / min for ...

Embodiment 2

[0064] Embodiment two, determine optimal carbon source

[0065] Carry out operation according to embodiment one, difference is: take the fermented medium before optimization as benchmark, investigate different carbon source (carbon source is respectively glucose, sucrose, glycerol, ethanol, sodium acetate, sodium citrate and starch) to SAM and GSH The impact of co-production fermentation, on this basis, the experimental results of co-production fermentation to prepare SAM and GSH, the results are shown in Table 2:

[0066] SAM output: 0~173.1mg / L; GSH output: 6.5~193.5mg / L;

[0067] The total co-production of SAM and GSH is 6.5-366.6mg / L;

[0068] Among them, the best carbon source is sucrose.

[0069] Table 2 Effects of carbon source types on co-production fermentation of SAM and GSH

[0070]

Embodiment 3

[0071] Embodiment three, determine optimal nitrogen source

[0072] Carry out operation according to embodiment one, difference is: take the fermented medium before optimization as benchmark, investigate different nitrogen sources (nitrogen sources are respectively yeast extract, beef extract, peptone, potassium nitrate, ammonium sulfate, urea and ammonium nitrate) to SAM The influence of co-production fermentation with GSH, on this basis, the experimental results of co-production fermentation to prepare SAM and GSH, the results are shown in Table 3:

[0073] SAM output: 41~146mg / L; GSH output: 57.5~167.7mg / L;

[0074] The total co-production of SAM and GSH is 90.5-308.7mg / L;

[0075] Among them, the best nitrogen source is ammonium sulfate.

[0076] Table 3 Effects of nitrogen source types on co-production fermentation of SAM and GSH

[0077]

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Abstract

The invention discloses an ethionine resistance Candida utilis and application thereof, wherein the Candida utilis SUGS-01 is preserved in China Center for Type Culture Collection (CCTCC) on December 9th, 2009, and the preservation number is CCTCC M 209298. The Candida utilis SUGS-01 is applied to produce SAM and GSH by co-production and fermentation, and the application comprises the following steps: taking the Candida utilis SUGS-01 as the original strain, and after slant culturing and seed culturing, fermenting for at least 24 hours; one litre of fermentation medium contains 35-36g of cane sugar, 10-12g of ammonium sulfate, 12-13g of monopotassium phosphate, 0.05-0.06g of magnesium sulfate and 4-5g of L-methionine; and the pH value of the fermentation medium is 5.5-6.0. Because adopting the combined action of UV mutagenesis and gamma-ray mutagenesis, the invention obtains the Candida utilis mutant strain which can co-produce SAM and GSH, and the obtained Candida utilis is used as production strains, has the advantages of wide growth nutrition spectrum, less production of ethanol during the fermentation process, strong capability to synthesize protein and the like, and has good industrial application prospect.

Description

technical field [0001] The invention belongs to microbial fermentation technology, and in particular relates to an ethionine-resistant Candida utilis and its application in co-production fermentation of S-adenosylmethionine and glutathione. Background technique [0002] S-adenosylmethionine (SAM) is an important small molecular substance widely present in biological cells, which was discovered by Cantoni in 1953. Studies have found that SAM, as a group provider and an enzyme inducer, participates in many key biochemical reactions, such as the methylation of lipids, proteins, and nucleic acids, the transsulfuration reaction, and the synthesis of polyamines. The physiological function of SAM in the cell involves: the methyl donor in the methyl reaction; the generation of methylthio adenosine and homoserine; the donor of the aminobutyl group of tRNA; the donor of the amino acid chain in the synthesis of vitamin H; Donor of glycoside moiety; inhibition of cytolysin-2,3-aminoall...

Claims

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Application Information

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IPC IPC(8): C12N1/16C12P19/40C12P21/02C12R1/72
Inventor 王大慧邵娜卫功元
Owner SUZHOU UNIV
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